Hydrogen fluoride catalyzed migration of side chain protecting groups onto Fmoc during solid phase peptide synthesis. Characterization by CF-FAB analysis of carboxypeptidase digestions and NMR spectroscopy

The solid-phase synthesis of the N alpha-Fmoc analog of protein kinase C substrate (PKCS, Lys-Arg-Ala-Lys-Ala-Lys-Thr-Thr-Lys-Lys-Arg) was characterized by low recovery from the resin and the concomitant appearance of four impurities. FAB-MS revealed molecular weights for two of these impurities tha...

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Veröffentlicht in:	International journal of peptide and protein research 1992-12, Vol.40 (6), p.538-545
Hauptverfasser:	Grode, S H, Strother, D S, Runge, T A, Dobrowolski, P J
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Amino Acids - chemistry Carbon Radioisotopes Carboxypeptidases - chemistry Fluorenes - chemistry Hydrofluoric Acid - chemistry Magnetic Resonance Spectroscopy - methods Molecular Sequence Data Oligopeptides - chemical synthesis Oligopeptides - isolation & purification Peptides - chemical synthesis Peptides - isolation & purification Spectrometry, Mass, Fast Atom Bombardment
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