Protein synthesis inhibitory activity in culture filtrates from new strains of Streptomyces isolated from Brazilian tropical soils
Aims: To investigate the effect of the culture supernatants from three newly isolated Streptomyces strains, 221, 235 and 606 on eukaryotic cells. Methods and Results: Cell lines were treated with the culture filtrates and assayed for protein synthesis by metabolic labelling, followed by sodium dodec...
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| Veröffentlicht in: | Letters in applied microbiology 2003-01, Vol.37 (2), p.138-143 |
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| creator | Reis, S.A. Costa, L.V. Cavalcanti, E.D.C. Giambiagi‐deMarval, M. Semêdo, L.T.A.S. Coelho, R.R.R. Moussatché, N. Damaso, C.R.A. |
| description | Aims: To investigate the effect of the culture supernatants from three newly isolated Streptomyces strains, 221, 235 and 606 on eukaryotic cells.
Methods and Results: Cell lines were treated with the culture filtrates and assayed for protein synthesis by metabolic labelling, followed by sodium dodecyl sulphate‐polyacrylamide gel electrophoresis analysis. RNA synthesis was investigated by [5‐3H]uridine incorporation. The three culture filtrates presented a strong inhibitory activity, reducing total protein synthesis of different eukaryotic cell lines by more than 85%. No effect on cellular RNA synthesis was detected. The culture filtrates did not affect the growth of the prokaryotic cells tested.
Conclusions: These new Streptomyces strains, recently isolated from Brazilian tropical soils, produce molecule(s) with inhibitory activity specific to eukaryote protein synthesis.
Significance and Impact of the Study: Streptomyces strains 221, 235 and 606, probably representing new species, might produce new bioactive compound(s), and can be used as valuable tools to study the protein synthesis pathway in eukaryotes. |
| doi_str_mv | 10.1046/j.1472-765X.2003.01363.x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_73475273</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>73475273</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4263-c2dd9bad0125e038d266028563845936f83a082ebc13332921cb8e42d493b7da3</originalsourceid><addsrcrecordid>eNqNkU1v1DAQQC0EotvCX0C-wC3BX3GcA4dSQUFaBBIgcbMcx1G98saLx6ENR345DruiRziNNX4z4_FDCFNSUyLky11NRcuqVjbfakYIrwnlktd3D9Dm78VDtCFMykqxRpyhc4AdIURR1j1GZ5SpppON3KBfn1LMzk8YlinfOPCA_XTje59jWrCx2f_weSk5bOeQ5-Tw6ENOJjvAY4p7PLlbDCXhJ8BxxJ9zcocc94stgIcYCjkcydfJ_PTBmwnnFA_emoAh-gBP0KPRBHBPT_ECfX375svVu2r78fr91eW2soJJXlk2DF1vBkJZ4whXQ1mOlD0kV6LpuBwVN0Qx11vKOWcdo7ZXTrBBdLxvB8Mv0Itj30OK32cHWe89WBeCmVycQbdctA1r-T9BqpRsO8EKqI6gTREguVEfkt-btGhK9CpK7_TqQ68-9CpK_xGl70rps9OMud-74b7wZKYAz0-AgfJVYzKT9XDPNUSShovCvTpytz645b8foLeXH9YT_w0VobGV</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>18867942</pqid></control><display><type>article</type><title>Protein synthesis inhibitory activity in culture filtrates from new strains of Streptomyces isolated from Brazilian tropical soils</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Oxford University Press Journals All Titles (1996-Current)</source><source>Wiley Online Library All Journals</source><source>Alma/SFX Local Collection</source><creator>Reis, S.A. ; Costa, L.V. ; Cavalcanti, E.D.C. ; Giambiagi‐deMarval, M. ; Semêdo, L.T.A.S. ; Coelho, R.R.R. ; Moussatché, N. ; Damaso, C.R.A.</creator><creatorcontrib>Reis, S.A. ; Costa, L.V. ; Cavalcanti, E.D.C. ; Giambiagi‐deMarval, M. ; Semêdo, L.T.A.S. ; Coelho, R.R.R. ; Moussatché, N. ; Damaso, C.R.A.</creatorcontrib><description>Aims: To investigate the effect of the culture supernatants from three newly isolated Streptomyces strains, 221, 235 and 606 on eukaryotic cells.
Methods and Results: Cell lines were treated with the culture filtrates and assayed for protein synthesis by metabolic labelling, followed by sodium dodecyl sulphate‐polyacrylamide gel electrophoresis analysis. RNA synthesis was investigated by [5‐3H]uridine incorporation. The three culture filtrates presented a strong inhibitory activity, reducing total protein synthesis of different eukaryotic cell lines by more than 85%. No effect on cellular RNA synthesis was detected. The culture filtrates did not affect the growth of the prokaryotic cells tested.
Conclusions: These new Streptomyces strains, recently isolated from Brazilian tropical soils, produce molecule(s) with inhibitory activity specific to eukaryote protein synthesis.
Significance and Impact of the Study: Streptomyces strains 221, 235 and 606, probably representing new species, might produce new bioactive compound(s), and can be used as valuable tools to study the protein synthesis pathway in eukaryotes.</description><identifier>ISSN: 0266-8254</identifier><identifier>EISSN: 1472-765X</identifier><identifier>EISSN: 1365-2673</identifier><identifier>DOI: 10.1046/j.1472-765X.2003.01363.x</identifier><identifier>PMID: 12859656</identifier><identifier>CODEN: LAMIE7</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>3T3 Cells ; actinomycetes ; Animals ; antimetabolic activity ; Biological and medical sciences ; biotechnology ; Brazil ; Cell Line ; Culture Media ; eukaryotic translation ; Fundamental and applied biological sciences. Psychology ; Humans ; Mice ; Microbiology ; Protein Biosynthesis - drug effects ; Protein Synthesis Inhibitors - isolation & purification ; Protein Synthesis Inhibitors - metabolism ; Protein Synthesis Inhibitors - pharmacology ; RNA - biosynthesis ; Soil Microbiology ; Streptomyces - drug effects ; Streptomyces - growth & development ; Streptomyces - isolation & purification ; Streptomyces - metabolism ; translation inhibitor ; Tropical Climate</subject><ispartof>Letters in applied microbiology, 2003-01, Vol.37 (2), p.138-143</ispartof><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4263-c2dd9bad0125e038d266028563845936f83a082ebc13332921cb8e42d493b7da3</citedby><cites>FETCH-LOGICAL-c4263-c2dd9bad0125e038d266028563845936f83a082ebc13332921cb8e42d493b7da3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1472-765X.2003.01363.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1472-765X.2003.01363.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,782,786,1419,27931,27932,45581,45582</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15060534$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12859656$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Reis, S.A.</creatorcontrib><creatorcontrib>Costa, L.V.</creatorcontrib><creatorcontrib>Cavalcanti, E.D.C.</creatorcontrib><creatorcontrib>Giambiagi‐deMarval, M.</creatorcontrib><creatorcontrib>Semêdo, L.T.A.S.</creatorcontrib><creatorcontrib>Coelho, R.R.R.</creatorcontrib><creatorcontrib>Moussatché, N.</creatorcontrib><creatorcontrib>Damaso, C.R.A.</creatorcontrib><title>Protein synthesis inhibitory activity in culture filtrates from new strains of Streptomyces isolated from Brazilian tropical soils</title><title>Letters in applied microbiology</title><addtitle>Lett Appl Microbiol</addtitle><description>Aims: To investigate the effect of the culture supernatants from three newly isolated Streptomyces strains, 221, 235 and 606 on eukaryotic cells.
Methods and Results: Cell lines were treated with the culture filtrates and assayed for protein synthesis by metabolic labelling, followed by sodium dodecyl sulphate‐polyacrylamide gel electrophoresis analysis. RNA synthesis was investigated by [5‐3H]uridine incorporation. The three culture filtrates presented a strong inhibitory activity, reducing total protein synthesis of different eukaryotic cell lines by more than 85%. No effect on cellular RNA synthesis was detected. The culture filtrates did not affect the growth of the prokaryotic cells tested.
Conclusions: These new Streptomyces strains, recently isolated from Brazilian tropical soils, produce molecule(s) with inhibitory activity specific to eukaryote protein synthesis.
Significance and Impact of the Study: Streptomyces strains 221, 235 and 606, probably representing new species, might produce new bioactive compound(s), and can be used as valuable tools to study the protein synthesis pathway in eukaryotes.</description><subject>3T3 Cells</subject><subject>actinomycetes</subject><subject>Animals</subject><subject>antimetabolic activity</subject><subject>Biological and medical sciences</subject><subject>biotechnology</subject><subject>Brazil</subject><subject>Cell Line</subject><subject>Culture Media</subject><subject>eukaryotic translation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Mice</subject><subject>Microbiology</subject><subject>Protein Biosynthesis - drug effects</subject><subject>Protein Synthesis Inhibitors - isolation & purification</subject><subject>Protein Synthesis Inhibitors - metabolism</subject><subject>Protein Synthesis Inhibitors - pharmacology</subject><subject>RNA - biosynthesis</subject><subject>Soil Microbiology</subject><subject>Streptomyces - drug effects</subject><subject>Streptomyces - growth & development</subject><subject>Streptomyces - isolation & purification</subject><subject>Streptomyces - metabolism</subject><subject>translation inhibitor</subject><subject>Tropical Climate</subject><issn>0266-8254</issn><issn>1472-765X</issn><issn>1365-2673</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1v1DAQQC0EotvCX0C-wC3BX3GcA4dSQUFaBBIgcbMcx1G98saLx6ENR345DruiRziNNX4z4_FDCFNSUyLky11NRcuqVjbfakYIrwnlktd3D9Dm78VDtCFMykqxRpyhc4AdIURR1j1GZ5SpppON3KBfn1LMzk8YlinfOPCA_XTje59jWrCx2f_weSk5bOeQ5-Tw6ENOJjvAY4p7PLlbDCXhJ8BxxJ9zcocc94stgIcYCjkcydfJ_PTBmwnnFA_emoAh-gBP0KPRBHBPT_ECfX375svVu2r78fr91eW2soJJXlk2DF1vBkJZ4whXQ1mOlD0kV6LpuBwVN0Qx11vKOWcdo7ZXTrBBdLxvB8Mv0Itj30OK32cHWe89WBeCmVycQbdctA1r-T9BqpRsO8EKqI6gTREguVEfkt-btGhK9CpK7_TqQ68-9CpK_xGl70rps9OMud-74b7wZKYAz0-AgfJVYzKT9XDPNUSShovCvTpytz645b8foLeXH9YT_w0VobGV</recordid><startdate>20030101</startdate><enddate>20030101</enddate><creator>Reis, S.A.</creator><creator>Costa, L.V.</creator><creator>Cavalcanti, E.D.C.</creator><creator>Giambiagi‐deMarval, M.</creator><creator>Semêdo, L.T.A.S.</creator><creator>Coelho, R.R.R.</creator><creator>Moussatché, N.</creator><creator>Damaso, C.R.A.</creator><general>Blackwell Science Ltd</general><general>Blackwell Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20030101</creationdate><title>Protein synthesis inhibitory activity in culture filtrates from new strains of Streptomyces isolated from Brazilian tropical soils</title><author>Reis, S.A. ; Costa, L.V. ; Cavalcanti, E.D.C. ; Giambiagi‐deMarval, M. ; Semêdo, L.T.A.S. ; Coelho, R.R.R. ; Moussatché, N. ; Damaso, C.R.A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4263-c2dd9bad0125e038d266028563845936f83a082ebc13332921cb8e42d493b7da3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>3T3 Cells</topic><topic>actinomycetes</topic><topic>Animals</topic><topic>antimetabolic activity</topic><topic>Biological and medical sciences</topic><topic>biotechnology</topic><topic>Brazil</topic><topic>Cell Line</topic><topic>Culture Media</topic><topic>eukaryotic translation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Mice</topic><topic>Microbiology</topic><topic>Protein Biosynthesis - drug effects</topic><topic>Protein Synthesis Inhibitors - isolation & purification</topic><topic>Protein Synthesis Inhibitors - metabolism</topic><topic>Protein Synthesis Inhibitors - pharmacology</topic><topic>RNA - biosynthesis</topic><topic>Soil Microbiology</topic><topic>Streptomyces - drug effects</topic><topic>Streptomyces - growth & development</topic><topic>Streptomyces - isolation & purification</topic><topic>Streptomyces - metabolism</topic><topic>translation inhibitor</topic><topic>Tropical Climate</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Reis, S.A.</creatorcontrib><creatorcontrib>Costa, L.V.</creatorcontrib><creatorcontrib>Cavalcanti, E.D.C.</creatorcontrib><creatorcontrib>Giambiagi‐deMarval, M.</creatorcontrib><creatorcontrib>Semêdo, L.T.A.S.</creatorcontrib><creatorcontrib>Coelho, R.R.R.</creatorcontrib><creatorcontrib>Moussatché, N.</creatorcontrib><creatorcontrib>Damaso, C.R.A.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Letters in applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Reis, S.A.</au><au>Costa, L.V.</au><au>Cavalcanti, E.D.C.</au><au>Giambiagi‐deMarval, M.</au><au>Semêdo, L.T.A.S.</au><au>Coelho, R.R.R.</au><au>Moussatché, N.</au><au>Damaso, C.R.A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Protein synthesis inhibitory activity in culture filtrates from new strains of Streptomyces isolated from Brazilian tropical soils</atitle><jtitle>Letters in applied microbiology</jtitle><addtitle>Lett Appl Microbiol</addtitle><date>2003-01-01</date><risdate>2003</risdate><volume>37</volume><issue>2</issue><spage>138</spage><epage>143</epage><pages>138-143</pages><issn>0266-8254</issn><eissn>1472-765X</eissn><eissn>1365-2673</eissn><coden>LAMIE7</coden><abstract>Aims: To investigate the effect of the culture supernatants from three newly isolated Streptomyces strains, 221, 235 and 606 on eukaryotic cells.
Methods and Results: Cell lines were treated with the culture filtrates and assayed for protein synthesis by metabolic labelling, followed by sodium dodecyl sulphate‐polyacrylamide gel electrophoresis analysis. RNA synthesis was investigated by [5‐3H]uridine incorporation. The three culture filtrates presented a strong inhibitory activity, reducing total protein synthesis of different eukaryotic cell lines by more than 85%. No effect on cellular RNA synthesis was detected. The culture filtrates did not affect the growth of the prokaryotic cells tested.
Conclusions: These new Streptomyces strains, recently isolated from Brazilian tropical soils, produce molecule(s) with inhibitory activity specific to eukaryote protein synthesis.
Significance and Impact of the Study: Streptomyces strains 221, 235 and 606, probably representing new species, might produce new bioactive compound(s), and can be used as valuable tools to study the protein synthesis pathway in eukaryotes.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>12859656</pmid><doi>10.1046/j.1472-765X.2003.01363.x</doi><tpages>6</tpages></addata></record> |
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| subjects | 3T3 Cells actinomycetes Animals antimetabolic activity Biological and medical sciences biotechnology Brazil Cell Line Culture Media eukaryotic translation Fundamental and applied biological sciences. Psychology Humans Mice Microbiology Protein Biosynthesis - drug effects Protein Synthesis Inhibitors - isolation & purification Protein Synthesis Inhibitors - metabolism Protein Synthesis Inhibitors - pharmacology RNA - biosynthesis Soil Microbiology Streptomyces - drug effects Streptomyces - growth & development Streptomyces - isolation & purification Streptomyces - metabolism translation inhibitor Tropical Climate |
| title | Protein synthesis inhibitory activity in culture filtrates from new strains of Streptomyces isolated from Brazilian tropical soils |
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