Tropomodulin binds to filensin intermediate filaments
Tropomodulin (Tmod) is an actin filament pointed end capping protein found in the membrane skeleton of lens fiber cells. We demonstrate that Tmod4 is able to bind the lens-specific intermediate filament protein, filensin, in either co-sedimentation or solid phase binding assays in a saturable fashio...
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| Veröffentlicht in: | FEBS letters 2003-07, Vol.547 (1), p.228-232 |
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| container_title | FEBS letters |
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| creator | Fischer, R.S. Quinlan, R.A. Fowler, V.M. |
| description | Tropomodulin (Tmod) is an actin filament pointed end capping protein found in the membrane skeleton of lens fiber cells. We demonstrate that Tmod4 is able to bind the lens-specific intermediate filament protein, filensin, in either co-sedimentation or solid phase binding assays in a saturable fashion, but with low affinity and stoichiometry. Furthermore, Tmod4 does not bind the 53 kDa rod domain of filensin, nor to CP49, the obligate assembly partner of filensin. Finally, the binding of filensin to Tmod4 does not inhibit the actin capping activity of Tmod4 in vitro, suggesting that the two functions are not mutually exclusive. |
| doi_str_mv | 10.1016/S0014-5793(03)00711-7 |
| format | Article |
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We demonstrate that Tmod4 is able to bind the lens-specific intermediate filament protein, filensin, in either co-sedimentation or solid phase binding assays in a saturable fashion, but with low affinity and stoichiometry. Furthermore, Tmod4 does not bind the 53 kDa rod domain of filensin, nor to CP49, the obligate assembly partner of filensin. Finally, the binding of filensin to Tmod4 does not inhibit the actin capping activity of Tmod4 in vitro, suggesting that the two functions are not mutually exclusive.</description><subject>Actin</subject><subject>Actin Cytoskeleton - metabolism</subject><subject>Animals</subject><subject>Binding Sites</subject><subject>BSA, bovine serum albumin</subject><subject>Carrier Proteins - metabolism</subject><subject>Cattle</subject><subject>Cytoskeleton</subject><subject>Eye Proteins - metabolism</subject><subject>Intermediate filament</subject><subject>Intermediate Filament Proteins - metabolism</subject><subject>Intermediate Filaments - metabolism</subject><subject>Kinetics</subject><subject>Lens</subject><subject>Lens, Crystalline - metabolism</subject><subject>Microfilament Proteins</subject><subject>Molecular Weight</subject><subject>Protein Binding</subject><subject>Tropomodulin</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkF9LwzAUxYMobk4_grIn0YfqTZM07ZPomE4Y-OB8Ds2fQqRtZtIp-_am69BHhUDIueeee_ND6BzDDQac3b4CYJowXpArINcAHOOEH6AxzjlJCM3yQzT-sYzQSQjvEN85Lo7RCKd5BhQXY8RW3q1d4_Smtu1U2laHaeemla1NG6Ji2874xmhbdqZXy8a0XThFR1VZB3O2vyfo7XG-mi2S5cvT8-x-mShGCp5gyTBIwLLiqZGasYwVGJSWFeUpJYoznRLGWVVoJqmCFGTOWUENoYTFFckEXQ65a-8-NiZ0orFBmbouW-M2QXBCOS0yEo1sMCrvQvCmEmtvm9JvBQbR8xI7XqKHISCenldsn6CL_YCNjL_87doDiobFYPiKRLb_SxWP84d0V-kLQHZyP-tuiDKR2Kc1XgRlTasiXG9UJ7Szf2z7DTUSjIs</recordid><startdate>20030717</startdate><enddate>20030717</enddate><creator>Fischer, R.S.</creator><creator>Quinlan, R.A.</creator><creator>Fowler, V.M.</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20030717</creationdate><title>Tropomodulin binds to filensin intermediate filaments</title><author>Fischer, R.S. ; Quinlan, R.A. ; Fowler, V.M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5397-1b510b01bf72ebd5565910cdbf47243c75d23575f9d5b4c020b87594e34352863</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Actin</topic><topic>Actin Cytoskeleton - metabolism</topic><topic>Animals</topic><topic>Binding Sites</topic><topic>BSA, bovine serum albumin</topic><topic>Carrier Proteins - metabolism</topic><topic>Cattle</topic><topic>Cytoskeleton</topic><topic>Eye Proteins - metabolism</topic><topic>Intermediate filament</topic><topic>Intermediate Filament Proteins - metabolism</topic><topic>Intermediate Filaments - metabolism</topic><topic>Kinetics</topic><topic>Lens</topic><topic>Lens, Crystalline - metabolism</topic><topic>Microfilament Proteins</topic><topic>Molecular Weight</topic><topic>Protein Binding</topic><topic>Tropomodulin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fischer, R.S.</creatorcontrib><creatorcontrib>Quinlan, R.A.</creatorcontrib><creatorcontrib>Fowler, V.M.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fischer, R.S.</au><au>Quinlan, R.A.</au><au>Fowler, V.M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Tropomodulin binds to filensin intermediate filaments</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>2003-07-17</date><risdate>2003</risdate><volume>547</volume><issue>1</issue><spage>228</spage><epage>232</epage><pages>228-232</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>Tropomodulin (Tmod) is an actin filament pointed end capping protein found in the membrane skeleton of lens fiber cells. 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| language | eng |
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| source | MEDLINE; Wiley Free Content; ScienceDirect Journals (5 years ago - present); EZB-FREE-00999 freely available EZB journals; Wiley Online Library All Journals; Alma/SFX Local Collection |
| subjects | Actin Actin Cytoskeleton - metabolism Animals Binding Sites BSA, bovine serum albumin Carrier Proteins - metabolism Cattle Cytoskeleton Eye Proteins - metabolism Intermediate filament Intermediate Filament Proteins - metabolism Intermediate Filaments - metabolism Kinetics Lens Lens, Crystalline - metabolism Microfilament Proteins Molecular Weight Protein Binding Tropomodulin |
| title | Tropomodulin binds to filensin intermediate filaments |
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