Differentiation of endocrine myocardiocytes in the developing heart of the toad (Bufo arenarum Hensel)

The differentiation of endocrine myocardiocytes was investigated in the heart of developing toad Bufo arenarum Hensel, combining ultrastructural and immunocytochemical procedures. The distribution of immuno-reactive atrial natriuretic peptide (ANP) in the whole heart was appraised by light microscop...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:The International journal of developmental biology 1992-12, Vol.36 (4), p.537-542
Hauptverfasser: CASCO, V. G, PAZ, D, RUIZ, G, MALDONADO, C, PISANO, A, AOKI, A
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 542
container_issue 4
container_start_page 537
container_title The International journal of developmental biology
container_volume 36
creator CASCO, V. G
PAZ, D
RUIZ, G
MALDONADO, C
PISANO, A
AOKI, A
description The differentiation of endocrine myocardiocytes was investigated in the heart of developing toad Bufo arenarum Hensel, combining ultrastructural and immunocytochemical procedures. The distribution of immuno-reactive atrial natriuretic peptide (ANP) in the whole heart was appraised by light microscopy, applying biotin-streptavidin and immunofluorescence techniques. With the latter procedures ANP was first recognized at embryonic stage 22, in both atrium and ventricle. In the ensuing stages the ANP-reactivity became stronger in the atrium, while it became dimmer in the ventricle. At the end of the larval prometamorphic stage, atrial myocardiocytes acquired almost all the features of adult myoendocrine cells. At electron microscope level, small inclusions, about 110-120 nm in diameter, resembling secretory granules were found in myoendocrine cells beginning at embryonic stage 22. However, no immunogold labeling of ANP occurred until stage 25. The number of secretory granules diminished in the ventricles and increased in the atrium of the larval heart and at the end of the prometamorphic stage the atrial myoendocrine cells presented the ultrastructural characteristics of active secretory cells. The synthesis of ANP in larvae is enhanced at a critical period of development when the developing toad switches from an aquatic environment to terrestrial life. The cardiac hormones seem to play a key role in the regulation of the osmolarity of body fluids at this developmental stage.
format Article
fullrecord <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_73474151</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>73474151</sourcerecordid><originalsourceid>FETCH-LOGICAL-p235t-580f0ba5122e20f311211e004061b1ebc620bffa95aa6325760ad6f7adcd4883</originalsourceid><addsrcrecordid>eNo9kE9LxDAUxIMo67r6EYQcRPRQSNIkbY-6_llhwcvey2v74kbaZE1SYb-9FYun4c383hzmhCy5rnSWK1mckiUTXGZalOKcXMT4yaablcWCLLiolNJyScyTNQYDumQhWe-oNxRd59tgHdLh6FsInfXtMWGk1tG0R9rhN_b-YN0H3SOE9Pvz6ycPHb17HI2nMDVCGAe6QRexv78kZwb6iFezrsju5Xm33mTb99e39cM2O4hcpUyVzLAGFBcCBTM554JzZEwyzRuOTasFa4yBSgHoXKhCM-i0KaBrO1mW-Yrc_tUegv8aMaZ6sLHFvgeHfox1kctCcsUn8HoGx2bArj4EO0A41vMuU34z5xBb6E0A19r4j0lZcVWw_Afcw2zi</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>73474151</pqid></control><display><type>article</type><title>Differentiation of endocrine myocardiocytes in the developing heart of the toad (Bufo arenarum Hensel)</title><source>MEDLINE</source><source>Alma/SFX Local Collection</source><creator>CASCO, V. G ; PAZ, D ; RUIZ, G ; MALDONADO, C ; PISANO, A ; AOKI, A</creator><creatorcontrib>CASCO, V. G ; PAZ, D ; RUIZ, G ; MALDONADO, C ; PISANO, A ; AOKI, A</creatorcontrib><description>The differentiation of endocrine myocardiocytes was investigated in the heart of developing toad Bufo arenarum Hensel, combining ultrastructural and immunocytochemical procedures. The distribution of immuno-reactive atrial natriuretic peptide (ANP) in the whole heart was appraised by light microscopy, applying biotin-streptavidin and immunofluorescence techniques. With the latter procedures ANP was first recognized at embryonic stage 22, in both atrium and ventricle. In the ensuing stages the ANP-reactivity became stronger in the atrium, while it became dimmer in the ventricle. At the end of the larval prometamorphic stage, atrial myocardiocytes acquired almost all the features of adult myoendocrine cells. At electron microscope level, small inclusions, about 110-120 nm in diameter, resembling secretory granules were found in myoendocrine cells beginning at embryonic stage 22. However, no immunogold labeling of ANP occurred until stage 25. The number of secretory granules diminished in the ventricles and increased in the atrium of the larval heart and at the end of the prometamorphic stage the atrial myoendocrine cells presented the ultrastructural characteristics of active secretory cells. The synthesis of ANP in larvae is enhanced at a critical period of development when the developing toad switches from an aquatic environment to terrestrial life. The cardiac hormones seem to play a key role in the regulation of the osmolarity of body fluids at this developmental stage.</description><identifier>ISSN: 0214-6282</identifier><identifier>EISSN: 1696-3547</identifier><identifier>PMID: 1295564</identifier><language>eng</language><publisher>Bilbao: University of the Basque Country Press</publisher><subject>Animals ; Anura - embryology ; Atrial Natriuretic Factor - analysis ; Biological and medical sciences ; Cell Differentiation ; Fundamental and applied biological sciences. Psychology ; Heart ; Heart - embryology ; Immunohistochemistry ; Myocardium - cytology ; Myocardium - ultrastructure ; Vertebrates: cardiovascular system</subject><ispartof>The International journal of developmental biology, 1992-12, Vol.36 (4), p.537-542</ispartof><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=4491570$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1295564$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>CASCO, V. G</creatorcontrib><creatorcontrib>PAZ, D</creatorcontrib><creatorcontrib>RUIZ, G</creatorcontrib><creatorcontrib>MALDONADO, C</creatorcontrib><creatorcontrib>PISANO, A</creatorcontrib><creatorcontrib>AOKI, A</creatorcontrib><title>Differentiation of endocrine myocardiocytes in the developing heart of the toad (Bufo arenarum Hensel)</title><title>The International journal of developmental biology</title><addtitle>Int J Dev Biol</addtitle><description>The differentiation of endocrine myocardiocytes was investigated in the heart of developing toad Bufo arenarum Hensel, combining ultrastructural and immunocytochemical procedures. The distribution of immuno-reactive atrial natriuretic peptide (ANP) in the whole heart was appraised by light microscopy, applying biotin-streptavidin and immunofluorescence techniques. With the latter procedures ANP was first recognized at embryonic stage 22, in both atrium and ventricle. In the ensuing stages the ANP-reactivity became stronger in the atrium, while it became dimmer in the ventricle. At the end of the larval prometamorphic stage, atrial myocardiocytes acquired almost all the features of adult myoendocrine cells. At electron microscope level, small inclusions, about 110-120 nm in diameter, resembling secretory granules were found in myoendocrine cells beginning at embryonic stage 22. However, no immunogold labeling of ANP occurred until stage 25. The number of secretory granules diminished in the ventricles and increased in the atrium of the larval heart and at the end of the prometamorphic stage the atrial myoendocrine cells presented the ultrastructural characteristics of active secretory cells. The synthesis of ANP in larvae is enhanced at a critical period of development when the developing toad switches from an aquatic environment to terrestrial life. The cardiac hormones seem to play a key role in the regulation of the osmolarity of body fluids at this developmental stage.</description><subject>Animals</subject><subject>Anura - embryology</subject><subject>Atrial Natriuretic Factor - analysis</subject><subject>Biological and medical sciences</subject><subject>Cell Differentiation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Heart</subject><subject>Heart - embryology</subject><subject>Immunohistochemistry</subject><subject>Myocardium - cytology</subject><subject>Myocardium - ultrastructure</subject><subject>Vertebrates: cardiovascular system</subject><issn>0214-6282</issn><issn>1696-3547</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kE9LxDAUxIMo67r6EYQcRPRQSNIkbY-6_llhwcvey2v74kbaZE1SYb-9FYun4c383hzmhCy5rnSWK1mckiUTXGZalOKcXMT4yaablcWCLLiolNJyScyTNQYDumQhWe-oNxRd59tgHdLh6FsInfXtMWGk1tG0R9rhN_b-YN0H3SOE9Pvz6ycPHb17HI2nMDVCGAe6QRexv78kZwb6iFezrsju5Xm33mTb99e39cM2O4hcpUyVzLAGFBcCBTM554JzZEwyzRuOTasFa4yBSgHoXKhCM-i0KaBrO1mW-Yrc_tUegv8aMaZ6sLHFvgeHfox1kctCcsUn8HoGx2bArj4EO0A41vMuU34z5xBb6E0A19r4j0lZcVWw_Afcw2zi</recordid><startdate>19921201</startdate><enddate>19921201</enddate><creator>CASCO, V. G</creator><creator>PAZ, D</creator><creator>RUIZ, G</creator><creator>MALDONADO, C</creator><creator>PISANO, A</creator><creator>AOKI, A</creator><general>University of the Basque Country Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>19921201</creationdate><title>Differentiation of endocrine myocardiocytes in the developing heart of the toad (Bufo arenarum Hensel)</title><author>CASCO, V. G ; PAZ, D ; RUIZ, G ; MALDONADO, C ; PISANO, A ; AOKI, A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p235t-580f0ba5122e20f311211e004061b1ebc620bffa95aa6325760ad6f7adcd4883</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Animals</topic><topic>Anura - embryology</topic><topic>Atrial Natriuretic Factor - analysis</topic><topic>Biological and medical sciences</topic><topic>Cell Differentiation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Heart</topic><topic>Heart - embryology</topic><topic>Immunohistochemistry</topic><topic>Myocardium - cytology</topic><topic>Myocardium - ultrastructure</topic><topic>Vertebrates: cardiovascular system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>CASCO, V. G</creatorcontrib><creatorcontrib>PAZ, D</creatorcontrib><creatorcontrib>RUIZ, G</creatorcontrib><creatorcontrib>MALDONADO, C</creatorcontrib><creatorcontrib>PISANO, A</creatorcontrib><creatorcontrib>AOKI, A</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>The International journal of developmental biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>CASCO, V. G</au><au>PAZ, D</au><au>RUIZ, G</au><au>MALDONADO, C</au><au>PISANO, A</au><au>AOKI, A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differentiation of endocrine myocardiocytes in the developing heart of the toad (Bufo arenarum Hensel)</atitle><jtitle>The International journal of developmental biology</jtitle><addtitle>Int J Dev Biol</addtitle><date>1992-12-01</date><risdate>1992</risdate><volume>36</volume><issue>4</issue><spage>537</spage><epage>542</epage><pages>537-542</pages><issn>0214-6282</issn><eissn>1696-3547</eissn><abstract>The differentiation of endocrine myocardiocytes was investigated in the heart of developing toad Bufo arenarum Hensel, combining ultrastructural and immunocytochemical procedures. The distribution of immuno-reactive atrial natriuretic peptide (ANP) in the whole heart was appraised by light microscopy, applying biotin-streptavidin and immunofluorescence techniques. With the latter procedures ANP was first recognized at embryonic stage 22, in both atrium and ventricle. In the ensuing stages the ANP-reactivity became stronger in the atrium, while it became dimmer in the ventricle. At the end of the larval prometamorphic stage, atrial myocardiocytes acquired almost all the features of adult myoendocrine cells. At electron microscope level, small inclusions, about 110-120 nm in diameter, resembling secretory granules were found in myoendocrine cells beginning at embryonic stage 22. However, no immunogold labeling of ANP occurred until stage 25. The number of secretory granules diminished in the ventricles and increased in the atrium of the larval heart and at the end of the prometamorphic stage the atrial myoendocrine cells presented the ultrastructural characteristics of active secretory cells. The synthesis of ANP in larvae is enhanced at a critical period of development when the developing toad switches from an aquatic environment to terrestrial life. The cardiac hormones seem to play a key role in the regulation of the osmolarity of body fluids at this developmental stage.</abstract><cop>Bilbao</cop><pub>University of the Basque Country Press</pub><pmid>1295564</pmid><tpages>6</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0214-6282
ispartof The International journal of developmental biology, 1992-12, Vol.36 (4), p.537-542
issn 0214-6282
1696-3547
language eng
recordid cdi_proquest_miscellaneous_73474151
source MEDLINE; Alma/SFX Local Collection
subjects Animals
Anura - embryology
Atrial Natriuretic Factor - analysis
Biological and medical sciences
Cell Differentiation
Fundamental and applied biological sciences. Psychology
Heart
Heart - embryology
Immunohistochemistry
Myocardium - cytology
Myocardium - ultrastructure
Vertebrates: cardiovascular system
title Differentiation of endocrine myocardiocytes in the developing heart of the toad (Bufo arenarum Hensel)
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-05T05%3A17%3A06IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pubme&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Differentiation%20of%20endocrine%20myocardiocytes%20in%20the%20developing%20heart%20of%20the%20toad%20(Bufo%20arenarum%20Hensel)&rft.jtitle=The%20International%20journal%20of%20developmental%20biology&rft.au=CASCO,%20V.%20G&rft.date=1992-12-01&rft.volume=36&rft.issue=4&rft.spage=537&rft.epage=542&rft.pages=537-542&rft.issn=0214-6282&rft.eissn=1696-3547&rft_id=info:doi/&rft_dat=%3Cproquest_pubme%3E73474151%3C/proquest_pubme%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=73474151&rft_id=info:pmid/1295564&rfr_iscdi=true