Synthesis and turnover of membrane glycoconjugates in monolayer culture of pig and human epidermal cells
SUMMARY The regeneration and turnover of the surface glycoconjugates of trypsin‐prepared pig and human cultured epidermal cells have been determined using the glycoprotein precursors N‐acetyl‐D‐(I‐3H) glucosamine (3H‐NAG) and N‐(3H)‐acetyl‐D‐mannosamine (3H‐NAM). Sialic acid assays have been perform...
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| Veröffentlicht in: | British journal of dermatology (1951) 1981-06, Vol.104 (6), p.649-658 |
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| container_title | British journal of dermatology (1951) |
| container_volume | 104 |
| creator | DAVIES, HELEN W. TROTTER, M.D. |
| description | SUMMARY
The regeneration and turnover of the surface glycoconjugates of trypsin‐prepared pig and human cultured epidermal cells have been determined using the glycoprotein precursors N‐acetyl‐D‐(I‐3H) glucosamine (3H‐NAG) and N‐(3H)‐acetyl‐D‐mannosamine (3H‐NAM). Sialic acid assays have been performed on similar unlabelled cells. The major points which emerged from this study were:
1
Trypsin‐damaged cell surfaces are rapidly repaired, probably by normal membrane turnover. There was a 12% regeneration of sialic acid within 2 h and total resynthesis occurred within 24 h.
2
The presence of an internal membrane system, part of which also demonstrates turnover, probably contributes to the speed of surface membrane repair. Some of the glycoprotein/sialic acid of this internal membrane system (30%) remains bound for a considerable length of time.
3
The membrane turnover maintains the cell in equilibrium so that total loss equals the synthesis of glycoprotein.
4
The equilibration of 3H‐NAG or 3H‐NAM uptake between 24 and 48 h is limited by the relative concentrations of glucose and labelled sugar in the medium at this time.
5
3H‐NAM was a more specific marker of glycoprotein than 3H‐NAG.
6
The results for human epidermal cells closely matched those for pig epidermal cells, indicating that pig cells can be used as a model for human cells. |
| doi_str_mv | 10.1111/j.1365-2133.1981.tb00751.x |
| format | Article |
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The regeneration and turnover of the surface glycoconjugates of trypsin‐prepared pig and human cultured epidermal cells have been determined using the glycoprotein precursors N‐acetyl‐D‐(I‐3H) glucosamine (3H‐NAG) and N‐(3H)‐acetyl‐D‐mannosamine (3H‐NAM). Sialic acid assays have been performed on similar unlabelled cells. The major points which emerged from this study were:
1
Trypsin‐damaged cell surfaces are rapidly repaired, probably by normal membrane turnover. There was a 12% regeneration of sialic acid within 2 h and total resynthesis occurred within 24 h.
2
The presence of an internal membrane system, part of which also demonstrates turnover, probably contributes to the speed of surface membrane repair. Some of the glycoprotein/sialic acid of this internal membrane system (30%) remains bound for a considerable length of time.
3
The membrane turnover maintains the cell in equilibrium so that total loss equals the synthesis of glycoprotein.
4
The equilibration of 3H‐NAG or 3H‐NAM uptake between 24 and 48 h is limited by the relative concentrations of glucose and labelled sugar in the medium at this time.
5
3H‐NAM was a more specific marker of glycoprotein than 3H‐NAG.
6
The results for human epidermal cells closely matched those for pig epidermal cells, indicating that pig cells can be used as a model for human cells.</description><identifier>ISSN: 0007-0963</identifier><identifier>EISSN: 1365-2133</identifier><identifier>DOI: 10.1111/j.1365-2133.1981.tb00751.x</identifier><identifier>PMID: 7248176</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Acetylglucosamine - metabolism ; Animals ; Cell Membrane - drug effects ; Cell Membrane - metabolism ; Cells, Cultured ; epidermis ; Epidermis - drug effects ; Epidermis - metabolism ; glycoproteins ; Glycoproteins - metabolism ; Hexosamines - metabolism ; Humans ; man ; membrane turnover ; Neuraminidase - pharmacology ; pigs ; Sialic Acids - metabolism ; Swine ; Trypsin - pharmacology</subject><ispartof>British journal of dermatology (1951), 1981-06, Vol.104 (6), p.649-658</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4389-c7b84423238c33d5ea4000cdd16997ea8bda1cf8422154dc2e2da9cf3b75bf183</citedby><cites>FETCH-LOGICAL-c4389-c7b84423238c33d5ea4000cdd16997ea8bda1cf8422154dc2e2da9cf3b75bf183</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-2133.1981.tb00751.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-2133.1981.tb00751.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7248176$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>DAVIES, HELEN W.</creatorcontrib><creatorcontrib>TROTTER, M.D.</creatorcontrib><title>Synthesis and turnover of membrane glycoconjugates in monolayer culture of pig and human epidermal cells</title><title>British journal of dermatology (1951)</title><addtitle>Br J Dermatol</addtitle><description>SUMMARY
The regeneration and turnover of the surface glycoconjugates of trypsin‐prepared pig and human cultured epidermal cells have been determined using the glycoprotein precursors N‐acetyl‐D‐(I‐3H) glucosamine (3H‐NAG) and N‐(3H)‐acetyl‐D‐mannosamine (3H‐NAM). Sialic acid assays have been performed on similar unlabelled cells. The major points which emerged from this study were:
1
Trypsin‐damaged cell surfaces are rapidly repaired, probably by normal membrane turnover. There was a 12% regeneration of sialic acid within 2 h and total resynthesis occurred within 24 h.
2
The presence of an internal membrane system, part of which also demonstrates turnover, probably contributes to the speed of surface membrane repair. Some of the glycoprotein/sialic acid of this internal membrane system (30%) remains bound for a considerable length of time.
3
The membrane turnover maintains the cell in equilibrium so that total loss equals the synthesis of glycoprotein.
4
The equilibration of 3H‐NAG or 3H‐NAM uptake between 24 and 48 h is limited by the relative concentrations of glucose and labelled sugar in the medium at this time.
5
3H‐NAM was a more specific marker of glycoprotein than 3H‐NAG.
6
The results for human epidermal cells closely matched those for pig epidermal cells, indicating that pig cells can be used as a model for human cells.</description><subject>Acetylglucosamine - metabolism</subject><subject>Animals</subject><subject>Cell Membrane - drug effects</subject><subject>Cell Membrane - metabolism</subject><subject>Cells, Cultured</subject><subject>epidermis</subject><subject>Epidermis - drug effects</subject><subject>Epidermis - metabolism</subject><subject>glycoproteins</subject><subject>Glycoproteins - metabolism</subject><subject>Hexosamines - metabolism</subject><subject>Humans</subject><subject>man</subject><subject>membrane turnover</subject><subject>Neuraminidase - pharmacology</subject><subject>pigs</subject><subject>Sialic Acids - metabolism</subject><subject>Swine</subject><subject>Trypsin - pharmacology</subject><issn>0007-0963</issn><issn>1365-2133</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1981</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkU-P0zAQxS0EWsrCR0CyOHBL8L_EDgckdoFdYAUIFjhajj1pU5K42Ak03x6HVr0ifLHkee83nnkIPaEkp-k82-aUl0XGKOc5rRTNx5oQWdB8fwetTqW7aEXSc0aqkt9HD2LcEkI5KcgZOpNMKCrLFdp8mYdxA7GN2AwOj1MY_C8I2De4h74OZgC87mbrrR-209qMEHE74N4PvjNzEtqpSyZYDLt2_ReymXozYNi1DkJvOmyh6-JDdK8xXYRHx_scfX3z-vbyOrv5ePX28uVNZgVXVWZlrYRgnHFlOXcFGJGGsM7RsqokGFU7Q22jBGO0EM4yYM5UtuG1LOqGKn6Onh64u-B_ThBH3bdx-UGaxE9RSy4kYyX5p5AWrKKK8SR8fhDa4GMM0OhdaHsTZk2JXvLQW70sXS9L10se-piH3ifz42OXqe7BnazHAFL9xaH-u-1g_g-yvnj3qhRVAmQHQBtH2J8AJvzQpeSy0N8_XOlvn9n7T9e3Qiv-B8WBq3I</recordid><startdate>198106</startdate><enddate>198106</enddate><creator>DAVIES, HELEN W.</creator><creator>TROTTER, M.D.</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>M7Z</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>198106</creationdate><title>Synthesis and turnover of membrane glycoconjugates in monolayer culture of pig and human epidermal cells</title><author>DAVIES, HELEN W. ; TROTTER, M.D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4389-c7b84423238c33d5ea4000cdd16997ea8bda1cf8422154dc2e2da9cf3b75bf183</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1981</creationdate><topic>Acetylglucosamine - metabolism</topic><topic>Animals</topic><topic>Cell Membrane - drug effects</topic><topic>Cell Membrane - metabolism</topic><topic>Cells, Cultured</topic><topic>epidermis</topic><topic>Epidermis - drug effects</topic><topic>Epidermis - metabolism</topic><topic>glycoproteins</topic><topic>Glycoproteins - metabolism</topic><topic>Hexosamines - metabolism</topic><topic>Humans</topic><topic>man</topic><topic>membrane turnover</topic><topic>Neuraminidase - pharmacology</topic><topic>pigs</topic><topic>Sialic Acids - metabolism</topic><topic>Swine</topic><topic>Trypsin - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>DAVIES, HELEN W.</creatorcontrib><creatorcontrib>TROTTER, M.D.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>British journal of dermatology (1951)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>DAVIES, HELEN W.</au><au>TROTTER, M.D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Synthesis and turnover of membrane glycoconjugates in monolayer culture of pig and human epidermal cells</atitle><jtitle>British journal of dermatology (1951)</jtitle><addtitle>Br J Dermatol</addtitle><date>1981-06</date><risdate>1981</risdate><volume>104</volume><issue>6</issue><spage>649</spage><epage>658</epage><pages>649-658</pages><issn>0007-0963</issn><eissn>1365-2133</eissn><abstract>SUMMARY
The regeneration and turnover of the surface glycoconjugates of trypsin‐prepared pig and human cultured epidermal cells have been determined using the glycoprotein precursors N‐acetyl‐D‐(I‐3H) glucosamine (3H‐NAG) and N‐(3H)‐acetyl‐D‐mannosamine (3H‐NAM). Sialic acid assays have been performed on similar unlabelled cells. The major points which emerged from this study were:
1
Trypsin‐damaged cell surfaces are rapidly repaired, probably by normal membrane turnover. There was a 12% regeneration of sialic acid within 2 h and total resynthesis occurred within 24 h.
2
The presence of an internal membrane system, part of which also demonstrates turnover, probably contributes to the speed of surface membrane repair. Some of the glycoprotein/sialic acid of this internal membrane system (30%) remains bound for a considerable length of time.
3
The membrane turnover maintains the cell in equilibrium so that total loss equals the synthesis of glycoprotein.
4
The equilibration of 3H‐NAG or 3H‐NAM uptake between 24 and 48 h is limited by the relative concentrations of glucose and labelled sugar in the medium at this time.
5
3H‐NAM was a more specific marker of glycoprotein than 3H‐NAG.
6
The results for human epidermal cells closely matched those for pig epidermal cells, indicating that pig cells can be used as a model for human cells.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>7248176</pmid><doi>10.1111/j.1365-2133.1981.tb00751.x</doi><tpages>10</tpages></addata></record> |
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| ispartof | British journal of dermatology (1951), 1981-06, Vol.104 (6), p.649-658 |
| issn | 0007-0963 1365-2133 |
| language | eng |
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| source | MEDLINE; Access via Wiley Online Library |
| subjects | Acetylglucosamine - metabolism Animals Cell Membrane - drug effects Cell Membrane - metabolism Cells, Cultured epidermis Epidermis - drug effects Epidermis - metabolism glycoproteins Glycoproteins - metabolism Hexosamines - metabolism Humans man membrane turnover Neuraminidase - pharmacology pigs Sialic Acids - metabolism Swine Trypsin - pharmacology |
| title | Synthesis and turnover of membrane glycoconjugates in monolayer culture of pig and human epidermal cells |
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