Neuropeptide Y is neuroproliferative for post‐natal hippocampal precursor cells
New neurones are produced in the adult hippocampus throughout life and are necessary for certain types of hippocampal learning. Little, however, is known about the control of hippocampal neurogenesis. We used primary hippocampal cultures from early post‐natal rats and neuropeptide Y Y1 receptor knoc...
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| Veröffentlicht in: | Journal of neurochemistry 2003-08, Vol.86 (3), p.646-659 |
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| container_title | Journal of neurochemistry |
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| creator | Howell, Owain W. Scharfman, Helen E. Herzog, Herbert Sundstrom, Lars E. Beck‐Sickinger, Annette Gray, William P. |
| description | New neurones are produced in the adult hippocampus throughout life and are necessary for certain types of hippocampal learning. Little, however, is known about the control of hippocampal neurogenesis. We used primary hippocampal cultures from early post‐natal rats and neuropeptide Y Y1 receptor knockout mice as well as selective neuropeptide Y receptor antagonists and agonists to demonstrate that neuropeptide Y is proliferative for nestin‐positive, sphere‐forming hippocampal precursor cells and β‐tubulin‐positive neuroblasts and that the neuroproliferative effect of neuropeptide Y is mediated via its Y1 receptor. Immunohistochemistry confirmed Y1 receptor staining on both nestin‐positive cells and β‐tubulin‐positive cells in culture and short pulse 5‐bromo‐2‐deoxyuridine studies demonstrated that neuropeptide Y has a proliferative effect on both cell types. These studies suggest that the proliferation of hippocampal neuroblasts and precursor cells is increased by neuropeptide Y and, therefore, that hippocampal learning and memory may be modulated by neuropeptide Y‐releasing interneurones. |
| doi_str_mv | 10.1046/j.1471-4159.2003.01895.x |
| format | Article |
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Little, however, is known about the control of hippocampal neurogenesis. We used primary hippocampal cultures from early post‐natal rats and neuropeptide Y Y1 receptor knockout mice as well as selective neuropeptide Y receptor antagonists and agonists to demonstrate that neuropeptide Y is proliferative for nestin‐positive, sphere‐forming hippocampal precursor cells and β‐tubulin‐positive neuroblasts and that the neuroproliferative effect of neuropeptide Y is mediated via its Y1 receptor. Immunohistochemistry confirmed Y1 receptor staining on both nestin‐positive cells and β‐tubulin‐positive cells in culture and short pulse 5‐bromo‐2‐deoxyuridine studies demonstrated that neuropeptide Y has a proliferative effect on both cell types. These studies suggest that the proliferation of hippocampal neuroblasts and precursor cells is increased by neuropeptide Y and, therefore, that hippocampal learning and memory may be modulated by neuropeptide Y‐releasing interneurones.</description><identifier>ISSN: 0022-3042</identifier><identifier>EISSN: 1471-4159</identifier><identifier>DOI: 10.1046/j.1471-4159.2003.01895.x</identifier><identifier>PMID: 12859678</identifier><identifier>CODEN: JONRA9</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>Animals ; Animals, Newborn ; Antigens, Differentiation - biosynthesis ; Biological and medical sciences ; Bromodeoxyuridine ; cell culture ; Cell cycle, cell proliferation ; Cell Differentiation - drug effects ; Cell Division - drug effects ; Cell physiology ; Cells, Cultured ; Dose-Response Relationship, Drug ; Fibroblast Growth Factor 2 - pharmacology ; Fundamental and applied biological sciences. Psychology ; hippocampus ; Hippocampus - cytology ; Hippocampus - drug effects ; Hippocampus - metabolism ; Intermediate Filament Proteins - biosynthesis ; Mice ; Mice, Knockout ; Molecular and cellular biology ; Nerve Tissue Proteins ; Nestin ; neurogenesis ; neuropeptide Y ; Neuropeptide Y - pharmacology ; Rats ; Rats, Wistar ; Receptors, Neuropeptide Y - deficiency ; Receptors, Neuropeptide Y - genetics ; Receptors, Neuropeptide Y - metabolism ; Spheroids, Cellular - cytology ; Spheroids, Cellular - drug effects ; stem cell ; Stem Cells - cytology ; Stem Cells - drug effects ; Stem Cells - metabolism ; transgenic mouse ; Tubulin - biosynthesis</subject><ispartof>Journal of neurochemistry, 2003-08, Vol.86 (3), p.646-659</ispartof><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5425-bf893a6716be0540e914ca70ebd0aef0528fdd5d431cf1bc1329d72873d4c08d3</citedby><cites>FETCH-LOGICAL-c5425-bf893a6716be0540e914ca70ebd0aef0528fdd5d431cf1bc1329d72873d4c08d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1046%2Fj.1471-4159.2003.01895.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1046%2Fj.1471-4159.2003.01895.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,1427,27901,27902,45550,45551,46384,46808</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14990900$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12859678$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Howell, Owain W.</creatorcontrib><creatorcontrib>Scharfman, Helen E.</creatorcontrib><creatorcontrib>Herzog, Herbert</creatorcontrib><creatorcontrib>Sundstrom, Lars E.</creatorcontrib><creatorcontrib>Beck‐Sickinger, Annette</creatorcontrib><creatorcontrib>Gray, William P.</creatorcontrib><title>Neuropeptide Y is neuroproliferative for post‐natal hippocampal precursor cells</title><title>Journal of neurochemistry</title><addtitle>J Neurochem</addtitle><description>New neurones are produced in the adult hippocampus throughout life and are necessary for certain types of hippocampal learning. Little, however, is known about the control of hippocampal neurogenesis. We used primary hippocampal cultures from early post‐natal rats and neuropeptide Y Y1 receptor knockout mice as well as selective neuropeptide Y receptor antagonists and agonists to demonstrate that neuropeptide Y is proliferative for nestin‐positive, sphere‐forming hippocampal precursor cells and β‐tubulin‐positive neuroblasts and that the neuroproliferative effect of neuropeptide Y is mediated via its Y1 receptor. Immunohistochemistry confirmed Y1 receptor staining on both nestin‐positive cells and β‐tubulin‐positive cells in culture and short pulse 5‐bromo‐2‐deoxyuridine studies demonstrated that neuropeptide Y has a proliferative effect on both cell types. These studies suggest that the proliferation of hippocampal neuroblasts and precursor cells is increased by neuropeptide Y and, therefore, that hippocampal learning and memory may be modulated by neuropeptide Y‐releasing interneurones.</description><subject>Animals</subject><subject>Animals, Newborn</subject><subject>Antigens, Differentiation - biosynthesis</subject><subject>Biological and medical sciences</subject><subject>Bromodeoxyuridine</subject><subject>cell culture</subject><subject>Cell cycle, cell proliferation</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell Division - drug effects</subject><subject>Cell physiology</subject><subject>Cells, Cultured</subject><subject>Dose-Response Relationship, Drug</subject><subject>Fibroblast Growth Factor 2 - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>hippocampus</subject><subject>Hippocampus - cytology</subject><subject>Hippocampus - drug effects</subject><subject>Hippocampus - metabolism</subject><subject>Intermediate Filament Proteins - biosynthesis</subject><subject>Mice</subject><subject>Mice, Knockout</subject><subject>Molecular and cellular biology</subject><subject>Nerve Tissue Proteins</subject><subject>Nestin</subject><subject>neurogenesis</subject><subject>neuropeptide Y</subject><subject>Neuropeptide Y - pharmacology</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Receptors, Neuropeptide Y - deficiency</subject><subject>Receptors, Neuropeptide Y - genetics</subject><subject>Receptors, Neuropeptide Y - metabolism</subject><subject>Spheroids, Cellular - cytology</subject><subject>Spheroids, Cellular - drug effects</subject><subject>stem cell</subject><subject>Stem Cells - cytology</subject><subject>Stem Cells - drug effects</subject><subject>Stem Cells - metabolism</subject><subject>transgenic mouse</subject><subject>Tubulin - biosynthesis</subject><issn>0022-3042</issn><issn>1471-4159</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkM1O3DAQx62qqGy3vEKVC9ySjj_y4QOHalWgFQJVag-cLMceq15lN6mdULj1EfqMPAkOu4IjPc1o_JvxXz9CMgoFBVF9WhdU1DQXtJQFA-AF0EaWxd0bsnh-eEsWAIzlHAQ7JO9jXAPQSlT0HTmkrCllVTcL8v0Kp9APOIzeYnaT-Zhtnyah77zDoEd_i5nrQzb0cXz4-2-rR91lv_ww9EZvhtQPAc0UYkIMdl38QA6c7iIe7euS_Dz78mN1kV9en39dfb7MTSlYmbeukVxXNa1ahFIASiqMrgFbCxodlKxx1pZWcGocbQ3lTNqaNTW3wkBj-ZKc7O6mqL8njKPa-Dgn0Fvsp6hqnkyApK-CSZ2gUtQJbHagCX2MAZ0agt_ocK8oqNm7WqtZr5r1qtm7evKu7tLqx_0fU7tB-7K4F52A4z2go9GdC3prfHzhhJQg08UlOd1xf3yH9_8dQH27Ws0dfwToa6Ap</recordid><startdate>200308</startdate><enddate>200308</enddate><creator>Howell, Owain W.</creator><creator>Scharfman, Helen E.</creator><creator>Herzog, Herbert</creator><creator>Sundstrom, Lars E.</creator><creator>Beck‐Sickinger, Annette</creator><creator>Gray, William P.</creator><general>Blackwell Science Ltd</general><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>200308</creationdate><title>Neuropeptide Y is neuroproliferative for post‐natal hippocampal precursor cells</title><author>Howell, Owain W. ; Scharfman, Helen E. ; Herzog, Herbert ; Sundstrom, Lars E. ; Beck‐Sickinger, Annette ; Gray, William P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5425-bf893a6716be0540e914ca70ebd0aef0528fdd5d431cf1bc1329d72873d4c08d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>Animals, Newborn</topic><topic>Antigens, Differentiation - biosynthesis</topic><topic>Biological and medical sciences</topic><topic>Bromodeoxyuridine</topic><topic>cell culture</topic><topic>Cell cycle, cell proliferation</topic><topic>Cell Differentiation - drug effects</topic><topic>Cell Division - drug effects</topic><topic>Cell physiology</topic><topic>Cells, Cultured</topic><topic>Dose-Response Relationship, Drug</topic><topic>Fibroblast Growth Factor 2 - pharmacology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>hippocampus</topic><topic>Hippocampus - cytology</topic><topic>Hippocampus - drug effects</topic><topic>Hippocampus - metabolism</topic><topic>Intermediate Filament Proteins - biosynthesis</topic><topic>Mice</topic><topic>Mice, Knockout</topic><topic>Molecular and cellular biology</topic><topic>Nerve Tissue Proteins</topic><topic>Nestin</topic><topic>neurogenesis</topic><topic>neuropeptide Y</topic><topic>Neuropeptide Y - pharmacology</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Receptors, Neuropeptide Y - deficiency</topic><topic>Receptors, Neuropeptide Y - genetics</topic><topic>Receptors, Neuropeptide Y - metabolism</topic><topic>Spheroids, Cellular - cytology</topic><topic>Spheroids, Cellular - drug effects</topic><topic>stem cell</topic><topic>Stem Cells - cytology</topic><topic>Stem Cells - drug effects</topic><topic>Stem Cells - metabolism</topic><topic>transgenic mouse</topic><topic>Tubulin - biosynthesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Howell, Owain W.</creatorcontrib><creatorcontrib>Scharfman, Helen E.</creatorcontrib><creatorcontrib>Herzog, Herbert</creatorcontrib><creatorcontrib>Sundstrom, Lars E.</creatorcontrib><creatorcontrib>Beck‐Sickinger, Annette</creatorcontrib><creatorcontrib>Gray, William P.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of neurochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Howell, Owain W.</au><au>Scharfman, Helen E.</au><au>Herzog, Herbert</au><au>Sundstrom, Lars E.</au><au>Beck‐Sickinger, Annette</au><au>Gray, William P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Neuropeptide Y is neuroproliferative for post‐natal hippocampal precursor cells</atitle><jtitle>Journal of neurochemistry</jtitle><addtitle>J Neurochem</addtitle><date>2003-08</date><risdate>2003</risdate><volume>86</volume><issue>3</issue><spage>646</spage><epage>659</epage><pages>646-659</pages><issn>0022-3042</issn><eissn>1471-4159</eissn><coden>JONRA9</coden><abstract>New neurones are produced in the adult hippocampus throughout life and are necessary for certain types of hippocampal learning. Little, however, is known about the control of hippocampal neurogenesis. We used primary hippocampal cultures from early post‐natal rats and neuropeptide Y Y1 receptor knockout mice as well as selective neuropeptide Y receptor antagonists and agonists to demonstrate that neuropeptide Y is proliferative for nestin‐positive, sphere‐forming hippocampal precursor cells and β‐tubulin‐positive neuroblasts and that the neuroproliferative effect of neuropeptide Y is mediated via its Y1 receptor. Immunohistochemistry confirmed Y1 receptor staining on both nestin‐positive cells and β‐tubulin‐positive cells in culture and short pulse 5‐bromo‐2‐deoxyuridine studies demonstrated that neuropeptide Y has a proliferative effect on both cell types. These studies suggest that the proliferation of hippocampal neuroblasts and precursor cells is increased by neuropeptide Y and, therefore, that hippocampal learning and memory may be modulated by neuropeptide Y‐releasing interneurones.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>12859678</pmid><doi>10.1046/j.1471-4159.2003.01895.x</doi><tpages>14</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of neurochemistry, 2003-08, Vol.86 (3), p.646-659 |
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| subjects | Animals Animals, Newborn Antigens, Differentiation - biosynthesis Biological and medical sciences Bromodeoxyuridine cell culture Cell cycle, cell proliferation Cell Differentiation - drug effects Cell Division - drug effects Cell physiology Cells, Cultured Dose-Response Relationship, Drug Fibroblast Growth Factor 2 - pharmacology Fundamental and applied biological sciences. Psychology hippocampus Hippocampus - cytology Hippocampus - drug effects Hippocampus - metabolism Intermediate Filament Proteins - biosynthesis Mice Mice, Knockout Molecular and cellular biology Nerve Tissue Proteins Nestin neurogenesis neuropeptide Y Neuropeptide Y - pharmacology Rats Rats, Wistar Receptors, Neuropeptide Y - deficiency Receptors, Neuropeptide Y - genetics Receptors, Neuropeptide Y - metabolism Spheroids, Cellular - cytology Spheroids, Cellular - drug effects stem cell Stem Cells - cytology Stem Cells - drug effects Stem Cells - metabolism transgenic mouse Tubulin - biosynthesis |
| title | Neuropeptide Y is neuroproliferative for post‐natal hippocampal precursor cells |
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