Direct tissue analysis using matrix-assisted laser desorption/ionization mass spectrometry: practical aspects of sample preparation
Practical guidelines for the preparation of tissue sections for direct analysis by matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry are presented. Techniques for proper sample handling including tissue storage, sectioning and mounting are described. Emphasis is placed on optimiz...
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| Veröffentlicht in: | Journal of mass spectrometry. 2003-07, Vol.38 (7), p.699-708 |
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| container_end_page | 708 |
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| container_issue | 7 |
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| container_title | Journal of mass spectrometry. |
| container_volume | 38 |
| creator | Schwartz, Sarah A. Reyzer, Michelle L. Caprioli, Richard M. |
| description | Practical guidelines for the preparation of tissue sections for direct analysis by matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry are presented. Techniques for proper sample handling including tissue storage, sectioning and mounting are described. Emphasis is placed on optimizing matrix parameters such as the type of matrix molecule used, matrix concentration, and solvent composition. Several different techniques for matrix application are illustrated. Optimal instrument parameters and the necessity for advanced data analysis approaches with regards to direct tissue analysis are also discussed. Copyright © 2003 John Wiley & Sons, Ltd. |
| doi_str_mv | 10.1002/jms.505 |
| format | Article |
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Mass Spectrom</addtitle><description>Practical guidelines for the preparation of tissue sections for direct analysis by matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry are presented. Techniques for proper sample handling including tissue storage, sectioning and mounting are described. Emphasis is placed on optimizing matrix parameters such as the type of matrix molecule used, matrix concentration, and solvent composition. Several different techniques for matrix application are illustrated. Optimal instrument parameters and the necessity for advanced data analysis approaches with regards to direct tissue analysis are also discussed. Copyright © 2003 John Wiley & Sons, Ltd.</description><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects, investigation methods</subject><subject>imaging MS</subject><subject>Liver - chemistry</subject><subject>MALDI-MS</subject><subject>Mice</subject><subject>Proteins</subject><subject>Proteins - analysis</subject><subject>Rats</subject><subject>sample preparation</subject><subject>Specimen Handling - methods</subject><subject>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods</subject><subject>tissue analysis</subject><issn>1076-5174</issn><issn>1096-9888</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10U1v1DAQBmALgegHiH-AfIEeUFpn7cRxb6hAC7QgxEqVuFgTe4Jc8oUnEV2u_HG8zYqeOFge2Y9npNeMPcvFcS7E6uSmo-NCFA_Yfi5MmZmqqh5ua11mRa7VHjsguhFCGKPKx2wvX1WmKpXZZ3_ehIhu4lMgmpFDD-2GAvGZQv-ddzDFcJsBpaMJPW-BMHKPNMRxCkN_klb4DdsyWSJOY2oWhw6nuDnlYwQ3BQcth7sL4kPDCbqxxXSHI8S7p0_YowZawqe7_ZCt371dn11kl5_P35-9vsycqkSR-RqVLnIs8wYqB03plWwqX2tdi8J59ALAYK2NKJ3UWnrlhK9rXEEtG53LQ_ZyaTvG4eeMNNkukMO2hR6HmayWqixMJRM8WqCLA1HExo4xdBA3Nhd2G7dNcdsUd5LPdy3nukN_73b5JvBiB4BSDk2E3gW6d8ooJZRO7tXifoUWN_-bZz9cfV3GZove_srtPw3xhy211IW9_nRu11fqy4W-_mi_yb_Djqp9</recordid><startdate>200307</startdate><enddate>200307</enddate><creator>Schwartz, Sarah A.</creator><creator>Reyzer, Michelle L.</creator><creator>Caprioli, Richard M.</creator><general>John Wiley & Sons, Ltd</general><general>Wiley</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200307</creationdate><title>Direct tissue analysis using matrix-assisted laser desorption/ionization mass spectrometry: practical aspects of sample preparation</title><author>Schwartz, Sarah A. ; Reyzer, Michelle L. ; Caprioli, Richard M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4805-dbe4751e61fa8caf6d43f8db77b05cded0aa9eb7906c3773d4c0dbbe2ab3f713</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Analytical, structural and metabolic biochemistry</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects, investigation methods</topic><topic>imaging MS</topic><topic>Liver - chemistry</topic><topic>MALDI-MS</topic><topic>Mice</topic><topic>Proteins</topic><topic>Proteins - analysis</topic><topic>Rats</topic><topic>sample preparation</topic><topic>Specimen Handling - methods</topic><topic>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods</topic><topic>tissue analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Schwartz, Sarah A.</creatorcontrib><creatorcontrib>Reyzer, Michelle L.</creatorcontrib><creatorcontrib>Caprioli, Richard M.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of mass spectrometry.</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Schwartz, Sarah A.</au><au>Reyzer, Michelle L.</au><au>Caprioli, Richard M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Direct tissue analysis using matrix-assisted laser desorption/ionization mass spectrometry: practical aspects of sample preparation</atitle><jtitle>Journal of mass spectrometry.</jtitle><addtitle>J. Mass Spectrom</addtitle><date>2003-07</date><risdate>2003</risdate><volume>38</volume><issue>7</issue><spage>699</spage><epage>708</epage><pages>699-708</pages><issn>1076-5174</issn><eissn>1096-9888</eissn><abstract>Practical guidelines for the preparation of tissue sections for direct analysis by matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry are presented. Techniques for proper sample handling including tissue storage, sectioning and mounting are described. Emphasis is placed on optimizing matrix parameters such as the type of matrix molecule used, matrix concentration, and solvent composition. Several different techniques for matrix application are illustrated. Optimal instrument parameters and the necessity for advanced data analysis approaches with regards to direct tissue analysis are also discussed. 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| ispartof | Journal of mass spectrometry., 2003-07, Vol.38 (7), p.699-708 |
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| language | eng |
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| source | MEDLINE; Access via Wiley Online Library |
| subjects | Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Fundamental and applied biological sciences. Psychology General aspects, investigation methods imaging MS Liver - chemistry MALDI-MS Mice Proteins Proteins - analysis Rats sample preparation Specimen Handling - methods Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods tissue analysis |
| title | Direct tissue analysis using matrix-assisted laser desorption/ionization mass spectrometry: practical aspects of sample preparation |
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