Cloning, expression, purification and crystallization of the N-domain from the alpha(2) subunit of the membrane-spanning Na,K-ATPase protein

The nucleotide-binding domain of the Na,K-ATPase ion pump was expressed with a His tag in Escherichia coli and purified. The soluble 24 kDa derivative consists of 214 amino-acid residues and was crystallized in the presence of NiCl(2). The crystals belong to space group F23, with unit-cell parameter...

Ausführliche Beschreibung

Bibliographische Detailangaben

Veröffentlicht in:	Acta crystallographica. Section D, Biological crystallography. Biological crystallography., 2003-07, Vol.59 (Pt 7), p.1259-1261
Hauptverfasser:	Haue, Lisbeth, Pedersen, Per A, Jorgensen, Peter L, Håkansson, Kjell O
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Binding Sites Cloning, Molecular Crystallization - methods Membrane Proteins - chemistry Nickel Protein Structure, Tertiary Protein Subunits - chemistry Sodium-Potassium-Exchanging ATPase - chemistry Swine Thimerosal X-Ray Diffraction - methods
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!