The Rh complex exports ammonium from human red blood cells
The Rh blood group system represents a major immunodominant protein complex on red blood cells (RBC). Recently, the Rh homologues RhAG and RhCG were shown to promote ammonium ion transport in yeast. In this study, we showed that also in RBC the human Rh complex functions as an exporter of ammonium i...
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| Veröffentlicht in: | British journal of haematology 2003-07, Vol.122 (2), p.333-340 |
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| container_title | British journal of haematology |
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| creator | Hemker, Mirte B. Cheroutre, Goedele Van Zwieten, Rob Maaskant‐van Wijk, Petra A. Roos, Dirk Loos, Johannes A. Van Der Schoot, C. Ellen Von Dem Borne, Albert E. G. Kr |
| description | The Rh blood group system represents a major immunodominant protein complex on red blood cells (RBC). Recently, the Rh homologues RhAG and RhCG were shown to promote ammonium ion transport in yeast. In this study, we showed that also in RBC the human Rh complex functions as an exporter of ammonium ions. We measured ammonium import during the incubation of RBC in a solution containing a radiolabelled analogue of NH4Cl (14C‐methyl‐NH3Cl). Rhnull cells of the regulator type (expressing no Rh complex proteins) accumulated significantly higher levels (P = 0·05) of radiolabelled methyl‐ammonium ions than normal RBC, at room temperature. Rhnull cells of the amorph type (expressing limited amounts of Rh complex proteins) accumulated an intermediate amount of methyl‐ammonium ions. To show that decreased ammonium export contributes to its accumulation, the release of intracellular methyl‐ammonium from the cells was measured over time. In 30 s, normal RBC released 87% of the intracellular methyl‐ammonium ions, whereas Rhnull cells of the regulator type released only 46%. We conclude that the Rh complex is involved in the export of ammonium from RBC. |
| doi_str_mv | 10.1046/j.1365-2141.2003.04425.x |
| format | Article |
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Rhnull cells of the amorph type (expressing limited amounts of Rh complex proteins) accumulated an intermediate amount of methyl‐ammonium ions. To show that decreased ammonium export contributes to its accumulation, the release of intracellular methyl‐ammonium from the cells was measured over time. In 30 s, normal RBC released 87% of the intracellular methyl‐ammonium ions, whereas Rhnull cells of the regulator type released only 46%. We conclude that the Rh complex is involved in the export of ammonium from RBC.</description><identifier>ISSN: 0007-1048</identifier><identifier>EISSN: 1365-2141</identifier><identifier>DOI: 10.1046/j.1365-2141.2003.04425.x</identifier><identifier>PMID: 12846905</identifier><identifier>CODEN: BJHEAL</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>ammonium ; Benzylamines - metabolism ; Biological and medical sciences ; Biological Transport ; Carbon Radioisotopes - metabolism ; Cells, Cultured ; Erythrocytes - metabolism ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Hematology ; Humans ; Immunohematology ; Iron - metabolism ; Methylamines - metabolism ; Quaternary Ammonium Compounds - metabolism ; red blood cell ; Red blood cell immunology ; Rh complex ; Rh-Hr Blood-Group System - metabolism ; RhAg ; transport ; Urea - metabolism</subject><ispartof>British journal of haematology, 2003-07, Vol.122 (2), p.333-340</ispartof><rights>2003 INIST-CNRS</rights><rights>Copyright Blackwell Scientific Publications Ltd. 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Ellen</creatorcontrib><creatorcontrib>Von Dem Borne, Albert E. G. Kr</creatorcontrib><title>The Rh complex exports ammonium from human red blood cells</title><title>British journal of haematology</title><addtitle>Br J Haematol</addtitle><description>The Rh blood group system represents a major immunodominant protein complex on red blood cells (RBC). Recently, the Rh homologues RhAG and RhCG were shown to promote ammonium ion transport in yeast. In this study, we showed that also in RBC the human Rh complex functions as an exporter of ammonium ions. We measured ammonium import during the incubation of RBC in a solution containing a radiolabelled analogue of NH4Cl (14C‐methyl‐NH3Cl). Rhnull cells of the regulator type (expressing no Rh complex proteins) accumulated significantly higher levels (P = 0·05) of radiolabelled methyl‐ammonium ions than normal RBC, at room temperature. Rhnull cells of the amorph type (expressing limited amounts of Rh complex proteins) accumulated an intermediate amount of methyl‐ammonium ions. To show that decreased ammonium export contributes to its accumulation, the release of intracellular methyl‐ammonium from the cells was measured over time. In 30 s, normal RBC released 87% of the intracellular methyl‐ammonium ions, whereas Rhnull cells of the regulator type released only 46%. We conclude that the Rh complex is involved in the export of ammonium from RBC.</description><subject>ammonium</subject><subject>Benzylamines - metabolism</subject><subject>Biological and medical sciences</subject><subject>Biological Transport</subject><subject>Carbon Radioisotopes - metabolism</subject><subject>Cells, Cultured</subject><subject>Erythrocytes - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Hematology</subject><subject>Humans</subject><subject>Immunohematology</subject><subject>Iron - metabolism</subject><subject>Methylamines - metabolism</subject><subject>Quaternary Ammonium Compounds - metabolism</subject><subject>red blood cell</subject><subject>Red blood cell immunology</subject><subject>Rh complex</subject><subject>Rh-Hr Blood-Group System - metabolism</subject><subject>RhAg</subject><subject>transport</subject><subject>Urea - metabolism</subject><issn>0007-1048</issn><issn>1365-2141</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkEtLxDAYRYMoOj7-ggRBd615JxVcqPhkQBBdhzRNmQ5NMyYWZ_69rTMouHKTBHLu910OABCjHCMmzuc5poJnBDOcE4RojhgjPF9ugcnPxzaYIIRkNgTUHthPaY4QpojjXbCHiWKiQHwCLl5nDr7MoA1-0boldMtFiB8JGu9D1_Qe1jF4OOu96WB0FSzbECpoXdumQ7BTmza5o819AN7ubl9vHrLp8_3jzdU0s0wSnjlkK1EIxYmViBIplSxtoQw2krtK0lIKZhTDRijDsRWc1UVtS-wULWlRY3oAztZzFzG89y59aN-ksYHpXOiTlpQJUlA5gCd_wHnoYzd007gY9ovhGCC1hmwMKUVX60VsvIkrjZEe5eq5Hh3q0aEe5epvuXo5RI838_vSu-o3uLE5AKcbwCRr2jqazjbpl2MFIQiP3OWa-2xat_p3AX399DC-6BeqapJ7</recordid><startdate>200307</startdate><enddate>200307</enddate><creator>Hemker, Mirte B.</creator><creator>Cheroutre, Goedele</creator><creator>Van Zwieten, Rob</creator><creator>Maaskant‐van Wijk, Petra A.</creator><creator>Roos, Dirk</creator><creator>Loos, Johannes A.</creator><creator>Van Der Schoot, C. Ellen</creator><creator>Von Dem Borne, Albert E. G. Kr</creator><general>Blackwell Science Ltd</general><general>Blackwell</general><general>Blackwell Publishing Ltd</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>200307</creationdate><title>The Rh complex exports ammonium from human red blood cells</title><author>Hemker, Mirte B. ; Cheroutre, Goedele ; Van Zwieten, Rob ; Maaskant‐van Wijk, Petra A. ; Roos, Dirk ; Loos, Johannes A. ; Van Der Schoot, C. Ellen ; Von Dem Borne, Albert E. G. 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Psychology</topic><topic>Fundamental immunology</topic><topic>Hematology</topic><topic>Humans</topic><topic>Immunohematology</topic><topic>Iron - metabolism</topic><topic>Methylamines - metabolism</topic><topic>Quaternary Ammonium Compounds - metabolism</topic><topic>red blood cell</topic><topic>Red blood cell immunology</topic><topic>Rh complex</topic><topic>Rh-Hr Blood-Group System - metabolism</topic><topic>RhAg</topic><topic>transport</topic><topic>Urea - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hemker, Mirte B.</creatorcontrib><creatorcontrib>Cheroutre, Goedele</creatorcontrib><creatorcontrib>Van Zwieten, Rob</creatorcontrib><creatorcontrib>Maaskant‐van Wijk, Petra A.</creatorcontrib><creatorcontrib>Roos, Dirk</creatorcontrib><creatorcontrib>Loos, Johannes A.</creatorcontrib><creatorcontrib>Van Der Schoot, C. Ellen</creatorcontrib><creatorcontrib>Von Dem Borne, Albert E. G. 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Kr</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Rh complex exports ammonium from human red blood cells</atitle><jtitle>British journal of haematology</jtitle><addtitle>Br J Haematol</addtitle><date>2003-07</date><risdate>2003</risdate><volume>122</volume><issue>2</issue><spage>333</spage><epage>340</epage><pages>333-340</pages><issn>0007-1048</issn><eissn>1365-2141</eissn><coden>BJHEAL</coden><abstract>The Rh blood group system represents a major immunodominant protein complex on red blood cells (RBC). Recently, the Rh homologues RhAG and RhCG were shown to promote ammonium ion transport in yeast. In this study, we showed that also in RBC the human Rh complex functions as an exporter of ammonium ions. We measured ammonium import during the incubation of RBC in a solution containing a radiolabelled analogue of NH4Cl (14C‐methyl‐NH3Cl). Rhnull cells of the regulator type (expressing no Rh complex proteins) accumulated significantly higher levels (P = 0·05) of radiolabelled methyl‐ammonium ions than normal RBC, at room temperature. Rhnull cells of the amorph type (expressing limited amounts of Rh complex proteins) accumulated an intermediate amount of methyl‐ammonium ions. To show that decreased ammonium export contributes to its accumulation, the release of intracellular methyl‐ammonium from the cells was measured over time. In 30 s, normal RBC released 87% of the intracellular methyl‐ammonium ions, whereas Rhnull cells of the regulator type released only 46%. We conclude that the Rh complex is involved in the export of ammonium from RBC.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>12846905</pmid><doi>10.1046/j.1365-2141.2003.04425.x</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Access via Wiley Online Library; Wiley Online Library (Open Access Collection) |
| subjects | ammonium Benzylamines - metabolism Biological and medical sciences Biological Transport Carbon Radioisotopes - metabolism Cells, Cultured Erythrocytes - metabolism Fundamental and applied biological sciences. Psychology Fundamental immunology Hematology Humans Immunohematology Iron - metabolism Methylamines - metabolism Quaternary Ammonium Compounds - metabolism red blood cell Red blood cell immunology Rh complex Rh-Hr Blood-Group System - metabolism RhAg transport Urea - metabolism |
| title | The Rh complex exports ammonium from human red blood cells |
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