Integrin receptors and ECM proteins involved in preferential adhesion of colon carcinoma cells to lung cells
To assess the putative role of extracellular matrix (ECM) proteins on lung cells interacting with integrin receptors on colon carcinoma cells, an in vitro adhesion assay was used to investigate these factors. Tumor necrosis factor (TNF)-α treatment of fetal lung cell line MRC 9, upregulated expressi...
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| Veröffentlicht in: | Cancer letters 2003-07, Vol.196 (2), p.217-227 |
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| creator | Karmakar, Sougata Mukherjee, Rama |
| description | To assess the putative role of extracellular matrix (ECM) proteins on lung cells interacting with integrin receptors on colon carcinoma cells, an in vitro adhesion assay was used to investigate these factors. Tumor necrosis factor (TNF)-α treatment of fetal lung cell line MRC 9, upregulated expression of ECM proteins and also supported enhanced adhesion of PTC colon carcinoma cells. Antibodies to ECM proteins significantly blocked this enhanced adhesion of PTC cells. Similarly, antibody blocking of β1 and β2 integrin receptors on PTC cells revealed the integrin receptors involved in this enhanced adhesion. β1 integrin receptors like α2β1, α4β1 and α5β1 on PTC cells were found interacting with their ECM ligands like fibronectin and laminin on TNF-α stimulated MRC 9 cells. Interestingly, PTC cells were found to constitutively express αLβ2, which is normally expressed by leukocytes. The data from the present study indicates that expression of multiple β1 and β2 integrins by colon carcinoma cells putatively allows these cells to successfully adhere to secondary sites like lungs. |
| doi_str_mv | 10.1016/S0304-3835(03)00208-8 |
| format | Article |
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Tumor necrosis factor (TNF)-α treatment of fetal lung cell line MRC 9, upregulated expression of ECM proteins and also supported enhanced adhesion of PTC colon carcinoma cells. Antibodies to ECM proteins significantly blocked this enhanced adhesion of PTC cells. Similarly, antibody blocking of β1 and β2 integrin receptors on PTC cells revealed the integrin receptors involved in this enhanced adhesion. β1 integrin receptors like α2β1, α4β1 and α5β1 on PTC cells were found interacting with their ECM ligands like fibronectin and laminin on TNF-α stimulated MRC 9 cells. Interestingly, PTC cells were found to constitutively express αLβ2, which is normally expressed by leukocytes. 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Tumor necrosis factor (TNF)-α treatment of fetal lung cell line MRC 9, upregulated expression of ECM proteins and also supported enhanced adhesion of PTC colon carcinoma cells. Antibodies to ECM proteins significantly blocked this enhanced adhesion of PTC cells. Similarly, antibody blocking of β1 and β2 integrin receptors on PTC cells revealed the integrin receptors involved in this enhanced adhesion. β1 integrin receptors like α2β1, α4β1 and α5β1 on PTC cells were found interacting with their ECM ligands like fibronectin and laminin on TNF-α stimulated MRC 9 cells. Interestingly, PTC cells were found to constitutively express αLβ2, which is normally expressed by leukocytes. The data from the present study indicates that expression of multiple β1 and β2 integrins by colon carcinoma cells putatively allows these cells to successfully adhere to secondary sites like lungs.</description><subject>Adenocarcinoma - metabolism</subject><subject>Antibiotics</subject><subject>Antibodies - pharmacology</subject><subject>Antibody blocking</subject><subject>Biological and medical sciences</subject><subject>Cell adhesion</subject><subject>Cell adhesion & migration</subject><subject>Cell Line</subject><subject>Colonic Neoplasms</subject><subject>Colorectal cancer</subject><subject>Extracellular matrix proteins</subject><subject>Extracellular Matrix Proteins - physiology</subject><subject>Flow cytometry</subject><subject>Humans</subject><subject>Integrins</subject><subject>Integrins - immunology</subject><subject>Integrins - metabolism</subject><subject>Ligands</subject><subject>Lung - physiology</subject><subject>Lungs</subject><subject>Medical sciences</subject><subject>Proteins</subject><subject>Tumor Cells, Cultured</subject><subject>Tumors</subject><issn>0304-3835</issn><issn>1872-7980</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUGLFDEQhYMo7rj6E5SAKHporaQ73emTyLDqwooH9RzSleo1S08yJt0D_nvTO4MLXpYcUhW-PF7VY-y5gHcCRPv-O9TQVLWu1Ruo3wJI0JV-wDZCd7Lqeg0P2eYfcsae5HwDAKrp1GN2JqRuQWrYsOkyzHSdfOCJkPZzTJnb4PjF9ivfpziTD5n7cIjTgVwpyiONlCjM3k7cul-UfQw8jhzjVAq0CX2IO8uRpinzOfJpCdfH7il7NNop07PTfc5-frr4sf1SXX37fLn9eFVho2CuyGGva0SJ2DvZKiF0M1pqR5IWtXQCBpKA1Lcoh97VsumwbXsc3NDLsRnrc_b6qFsm-L1Qns3O59WBDRSXbLq6aYVS4l5QaA3l6AK-_A-8iUsKZQgjFKi6bbp-lVNHClPMuSzK7JPf2fTHCDBrauY2NbNGYqA2t6mZVf3FSX0ZduTufp1iKsCrE2Az2mlMNqDPd1zTN7JYKNyHI0dluwdPyWT0FJCcL_HOxkV_j5W_saW0lw</recordid><startdate>20030710</startdate><enddate>20030710</enddate><creator>Karmakar, Sougata</creator><creator>Mukherjee, Rama</creator><general>Elsevier Ireland Ltd</general><general>Elsevier</general><general>Elsevier Limited</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TO</scope><scope>7U9</scope><scope>H94</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>7X8</scope></search><sort><creationdate>20030710</creationdate><title>Integrin receptors and ECM proteins involved in preferential adhesion of colon carcinoma cells to lung cells</title><author>Karmakar, Sougata ; Mukherjee, Rama</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c450t-edc983cc2cc9d2651184fae6fe2ac82d10be20ce96c2b9d3247c669cbdb92f4f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Adenocarcinoma - metabolism</topic><topic>Antibiotics</topic><topic>Antibodies - pharmacology</topic><topic>Antibody blocking</topic><topic>Biological and medical sciences</topic><topic>Cell adhesion</topic><topic>Cell adhesion & migration</topic><topic>Cell Line</topic><topic>Colonic Neoplasms</topic><topic>Colorectal cancer</topic><topic>Extracellular matrix proteins</topic><topic>Extracellular Matrix Proteins - physiology</topic><topic>Flow cytometry</topic><topic>Humans</topic><topic>Integrins</topic><topic>Integrins - immunology</topic><topic>Integrins - metabolism</topic><topic>Ligands</topic><topic>Lung - physiology</topic><topic>Lungs</topic><topic>Medical sciences</topic><topic>Proteins</topic><topic>Tumor Cells, Cultured</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Karmakar, Sougata</creatorcontrib><creatorcontrib>Mukherjee, Rama</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>MEDLINE - Academic</collection><jtitle>Cancer letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Karmakar, Sougata</au><au>Mukherjee, Rama</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Integrin receptors and ECM proteins involved in preferential adhesion of colon carcinoma cells to lung cells</atitle><jtitle>Cancer letters</jtitle><addtitle>Cancer Lett</addtitle><date>2003-07-10</date><risdate>2003</risdate><volume>196</volume><issue>2</issue><spage>217</spage><epage>227</epage><pages>217-227</pages><issn>0304-3835</issn><eissn>1872-7980</eissn><coden>CALEDQ</coden><abstract>To assess the putative role of extracellular matrix (ECM) proteins on lung cells interacting with integrin receptors on colon carcinoma cells, an in vitro adhesion assay was used to investigate these factors. Tumor necrosis factor (TNF)-α treatment of fetal lung cell line MRC 9, upregulated expression of ECM proteins and also supported enhanced adhesion of PTC colon carcinoma cells. Antibodies to ECM proteins significantly blocked this enhanced adhesion of PTC cells. Similarly, antibody blocking of β1 and β2 integrin receptors on PTC cells revealed the integrin receptors involved in this enhanced adhesion. β1 integrin receptors like α2β1, α4β1 and α5β1 on PTC cells were found interacting with their ECM ligands like fibronectin and laminin on TNF-α stimulated MRC 9 cells. Interestingly, PTC cells were found to constitutively express αLβ2, which is normally expressed by leukocytes. 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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Adenocarcinoma - metabolism Antibiotics Antibodies - pharmacology Antibody blocking Biological and medical sciences Cell adhesion Cell adhesion & migration Cell Line Colonic Neoplasms Colorectal cancer Extracellular matrix proteins Extracellular Matrix Proteins - physiology Flow cytometry Humans Integrins Integrins - immunology Integrins - metabolism Ligands Lung - physiology Lungs Medical sciences Proteins Tumor Cells, Cultured Tumors |
| title | Integrin receptors and ECM proteins involved in preferential adhesion of colon carcinoma cells to lung cells |
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