Cloning, expression and characterization of a family‐74 xyloglucanase from Thermobifida fusca

Thermobifida fusca xyloglucan‐specific endo‐β‐1,4‐glucanase (Xeg)74 and the Xeg74 catalytic domain (CD) were cloned, expressed in Escherichia coli, purified and characterized. This enzyme has a glycohydrolase family‐74 CD that is a specific xyloglucanase followed by a family‐2 carbohydrate binding m...

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Veröffentlicht in:	European journal of biochemistry 2003-07, Vol.270 (14), p.3083-3091
Hauptverfasser:	Irwin, Diana C., Cheng, Mark, Xiang, Bosong, Rose, Jocelyn K. C., Wilson, David B.
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetobacter - chemistry Actinomycetales - enzymology Actinomycetales - genetics Actinomycetales - growth & development Amino Acid Sequence Blotting, Western Carbohydrate Sequence Catalytic Domain Cell Wall - metabolism cellulase Cellulose - chemistry Cellulose - metabolism Cloning, Molecular Escherichia coli - metabolism Glucans Glycoside Hydrolases - chemistry Glycoside Hydrolases - genetics Glycoside Hydrolases - metabolism Hydrogen-Ion Concentration Hydrolysis inverting Lycopersicon esculentum - chemistry Molecular Sequence Data Nuclear Magnetic Resonance, Biomolecular Oligosaccharides - chemistry Oligosaccharides - metabolism plant cell walls Polysaccharides - metabolism Recombinant Proteins - genetics Recombinant Proteins - metabolism regulation Seeds - chemistry Tamarindus - chemistry Temperature Xylans xyloglucanase
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