Site-specific fluorescent derivatization and liquid chromatographic-mass spectrometric characterization of long R(3) IGF-I for bioanalytical applications

Recombinant Long R(3) IGF-I was derivatized with fluorescein isothiocyanate (FITC) at a single location by careful selection of reaction conditions (i.e. pH, and FITC/protein amino group ratio). High-performance liquid chromatography (LC) and electrospray mass spectrometry (MS) were used to confirm...

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Veröffentlicht in:	Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2003-08, Vol.793 (1), p.115-125
Hauptverfasser:	Harvey, Michael D, Banks, Peter R
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Chromatography, High Pressure Liquid - methods Fluorescent Dyes - chemistry Insulin-Like Growth Factor I - chemistry Mass Spectrometry - methods Molecular Sequence Data
Online-Zugang:	Volltext
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