INS(1,4,5)P3 induces Ca2+ release from brain microsomes loaded either by the Ca2+ ATPase or by the Na+/Ca2+ exchanger
In this study we investigated the release of Ca2+ in brain microsomes after Ca2+ loading by the Ca(2+)-ATPase or by the Na+/Ca2+ exchanger. The results show that in microsomes loaded with Ca2+ by the Ca(2+)-ATPase, Ins(1,4,5)P3 (5 microM) released 21 +/- 2% of the total Ca2+ accumulated, and that in...
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| Veröffentlicht in: | Cellular signalling 1992-11, Vol.4 (6), p.687-696 |
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| creator | CRISTOVAO, A. J CARVALHO, C. A. M |
| description | In this study we investigated the release of Ca2+ in brain microsomes after Ca2+ loading by the Ca(2+)-ATPase or by the Na+/Ca2+ exchanger. The results show that in microsomes loaded with Ca2+ by the Ca(2+)-ATPase, Ins(1,4,5)P3 (5 microM) released 21 +/- 2% of the total Ca2+ accumulated, and that in the microsomes loaded with Ca2+ by the Na+/Ca2+ exchanger, Ins(1,4,5)P3 released 28 +/- 3% of the total Ca2+ accumulated. These results suggest that receptors of Ins(1,4,5)P3 may be co-localized with the Na+/Ca2+ exchanger in the endoplasmic reticulum membrane or that there are Ins(1,4,5)P3 receptors in the plasma membrane where the Na+/Ca2+ exchanger is normally present, or both. We also found that Ins(1,4,5)P3 inhibited the Ca(2+)-ATPase by 33.7%, but that it had no significant effect on the Na+/Ca2+ exchanger. |
| doi_str_mv | 10.1016/0898-6568(92)90049-E |
| format | Article |
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J ; CARVALHO, C. A. M</creator><creatorcontrib>CRISTOVAO, A. J ; CARVALHO, C. A. M</creatorcontrib><description>In this study we investigated the release of Ca2+ in brain microsomes after Ca2+ loading by the Ca(2+)-ATPase or by the Na+/Ca2+ exchanger. The results show that in microsomes loaded with Ca2+ by the Ca(2+)-ATPase, Ins(1,4,5)P3 (5 microM) released 21 +/- 2% of the total Ca2+ accumulated, and that in the microsomes loaded with Ca2+ by the Na+/Ca2+ exchanger, Ins(1,4,5)P3 released 28 +/- 3% of the total Ca2+ accumulated. These results suggest that receptors of Ins(1,4,5)P3 may be co-localized with the Na+/Ca2+ exchanger in the endoplasmic reticulum membrane or that there are Ins(1,4,5)P3 receptors in the plasma membrane where the Na+/Ca2+ exchanger is normally present, or both. We also found that Ins(1,4,5)P3 inhibited the Ca(2+)-ATPase by 33.7%, but that it had no significant effect on the Na+/Ca2+ exchanger.</description><identifier>ISSN: 0898-6568</identifier><identifier>EISSN: 1873-3913</identifier><identifier>DOI: 10.1016/0898-6568(92)90049-E</identifier><identifier>PMID: 1489660</identifier><language>eng</language><publisher>Amsterdam: Elsevier</publisher><subject>Animals ; Biological and medical sciences ; Brain - drug effects ; Brain - metabolism ; Calcium - metabolism ; Calcium-Transporting ATPases - metabolism ; Carrier Proteins - metabolism ; Cell physiology ; Female ; Fundamental and applied biological sciences. Psychology ; In Vitro Techniques ; Inositol 1,4,5-Trisphosphate - pharmacology ; Male ; Microsomes - drug effects ; Microsomes - metabolism ; Molecular and cellular biology ; Rats ; Rats, Sprague-Dawley ; Responses to growth factors, tumor promotors, other factors ; Signal Transduction ; Sodium - metabolism ; Sodium-Calcium Exchanger</subject><ispartof>Cellular signalling, 1992-11, Vol.4 (6), p.687-696</ispartof><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c1780-7e321cd491f8f4f1d676259ddfdd1fa5b0157c7dbfc1288b325a54297392d343</citedby><cites>FETCH-LOGICAL-c1780-7e321cd491f8f4f1d676259ddfdd1fa5b0157c7dbfc1288b325a54297392d343</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4683836$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1489660$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>CRISTOVAO, A. J</creatorcontrib><creatorcontrib>CARVALHO, C. A. M</creatorcontrib><title>INS(1,4,5)P3 induces Ca2+ release from brain microsomes loaded either by the Ca2+ ATPase or by the Na+/Ca2+ exchanger</title><title>Cellular signalling</title><addtitle>Cell Signal</addtitle><description>In this study we investigated the release of Ca2+ in brain microsomes after Ca2+ loading by the Ca(2+)-ATPase or by the Na+/Ca2+ exchanger. The results show that in microsomes loaded with Ca2+ by the Ca(2+)-ATPase, Ins(1,4,5)P3 (5 microM) released 21 +/- 2% of the total Ca2+ accumulated, and that in the microsomes loaded with Ca2+ by the Na+/Ca2+ exchanger, Ins(1,4,5)P3 released 28 +/- 3% of the total Ca2+ accumulated. These results suggest that receptors of Ins(1,4,5)P3 may be co-localized with the Na+/Ca2+ exchanger in the endoplasmic reticulum membrane or that there are Ins(1,4,5)P3 receptors in the plasma membrane where the Na+/Ca2+ exchanger is normally present, or both. We also found that Ins(1,4,5)P3 inhibited the Ca(2+)-ATPase by 33.7%, but that it had no significant effect on the Na+/Ca2+ exchanger.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Brain - drug effects</subject><subject>Brain - metabolism</subject><subject>Calcium - metabolism</subject><subject>Calcium-Transporting ATPases - metabolism</subject><subject>Carrier Proteins - metabolism</subject><subject>Cell physiology</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>In Vitro Techniques</subject><subject>Inositol 1,4,5-Trisphosphate - pharmacology</subject><subject>Male</subject><subject>Microsomes - drug effects</subject><subject>Microsomes - metabolism</subject><subject>Molecular and cellular biology</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Responses to growth factors, tumor promotors, other factors</subject><subject>Signal Transduction</subject><subject>Sodium - metabolism</subject><subject>Sodium-Calcium Exchanger</subject><issn>0898-6568</issn><issn>1873-3913</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkFtLAzEUhIMoWi__QCEPIpZ2NbfNJo-lVC0UFex7yOaiK3vRpAv6793tlvo0cOabgTMAXGJ0hxHm90hIkfCUi1tJxhIhJpPFARhhkdGESkwPwWiPnIDTGD8Rwini5BgcYyYk52gE2uXz2y2esmk6fqWwqG1rXIRzTSYwuNLp6KAPTQXzoIsaVoUJTWyqDikbbZ2Frth8uADzX9jpkJutX_tYs78-68n91nE_5kPX7y6cgyOvy-gudnoG1g-L9fwpWb08LuezVWJwJlCSOUqwsUxiLzzz2PKMk1Ra663FXqd5909mMpt7g4kQOSWpThmRGZXEUkbPwM1Q-xWa79bFjaqKaFxZ6to1bVQZZYx1nR3IBrB_Lwbn1VcoKh1-FUaqH1v1S6p-SSWJ2o6tFl3satff5pWz_6Fh3c6_3vk6Gl36oGtTxD3GuKCCcvoHKfyDVA</recordid><startdate>199211</startdate><enddate>199211</enddate><creator>CRISTOVAO, A. 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M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1780-7e321cd491f8f4f1d676259ddfdd1fa5b0157c7dbfc1288b325a54297392d343</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Brain - drug effects</topic><topic>Brain - metabolism</topic><topic>Calcium - metabolism</topic><topic>Calcium-Transporting ATPases - metabolism</topic><topic>Carrier Proteins - metabolism</topic><topic>Cell physiology</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>In Vitro Techniques</topic><topic>Inositol 1,4,5-Trisphosphate - pharmacology</topic><topic>Male</topic><topic>Microsomes - drug effects</topic><topic>Microsomes - metabolism</topic><topic>Molecular and cellular biology</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Responses to growth factors, tumor promotors, other factors</topic><topic>Signal Transduction</topic><topic>Sodium - metabolism</topic><topic>Sodium-Calcium Exchanger</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>CRISTOVAO, A. J</creatorcontrib><creatorcontrib>CARVALHO, C. A. M</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Cellular signalling</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>CRISTOVAO, A. J</au><au>CARVALHO, C. A. M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>INS(1,4,5)P3 induces Ca2+ release from brain microsomes loaded either by the Ca2+ ATPase or by the Na+/Ca2+ exchanger</atitle><jtitle>Cellular signalling</jtitle><addtitle>Cell Signal</addtitle><date>1992-11</date><risdate>1992</risdate><volume>4</volume><issue>6</issue><spage>687</spage><epage>696</epage><pages>687-696</pages><issn>0898-6568</issn><eissn>1873-3913</eissn><abstract>In this study we investigated the release of Ca2+ in brain microsomes after Ca2+ loading by the Ca(2+)-ATPase or by the Na+/Ca2+ exchanger. The results show that in microsomes loaded with Ca2+ by the Ca(2+)-ATPase, Ins(1,4,5)P3 (5 microM) released 21 +/- 2% of the total Ca2+ accumulated, and that in the microsomes loaded with Ca2+ by the Na+/Ca2+ exchanger, Ins(1,4,5)P3 released 28 +/- 3% of the total Ca2+ accumulated. These results suggest that receptors of Ins(1,4,5)P3 may be co-localized with the Na+/Ca2+ exchanger in the endoplasmic reticulum membrane or that there are Ins(1,4,5)P3 receptors in the plasma membrane where the Na+/Ca2+ exchanger is normally present, or both. We also found that Ins(1,4,5)P3 inhibited the Ca(2+)-ATPase by 33.7%, but that it had no significant effect on the Na+/Ca2+ exchanger.</abstract><cop>Amsterdam</cop><pub>Elsevier</pub><pmid>1489660</pmid><doi>10.1016/0898-6568(92)90049-E</doi><tpages>10</tpages></addata></record> |
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| ispartof | Cellular signalling, 1992-11, Vol.4 (6), p.687-696 |
| issn | 0898-6568 1873-3913 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Biological and medical sciences Brain - drug effects Brain - metabolism Calcium - metabolism Calcium-Transporting ATPases - metabolism Carrier Proteins - metabolism Cell physiology Female Fundamental and applied biological sciences. Psychology In Vitro Techniques Inositol 1,4,5-Trisphosphate - pharmacology Male Microsomes - drug effects Microsomes - metabolism Molecular and cellular biology Rats Rats, Sprague-Dawley Responses to growth factors, tumor promotors, other factors Signal Transduction Sodium - metabolism Sodium-Calcium Exchanger |
| title | INS(1,4,5)P3 induces Ca2+ release from brain microsomes loaded either by the Ca2+ ATPase or by the Na+/Ca2+ exchanger |
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