Enzyme-linked immunosorbent assay of the detection of antibodies to exocellular proteins of Staphylococcus aureus in bone infection
An enzyme-linked immunosorbent assay (ELISA) was developed for measurement of the antibody response to exocellular protein antigens of Staphylococcus aureus. The wells of a microtitre plate were coated with the exocellular proteins present in the tryptic soya broth growth medium from a stationary ph...
Gespeichert in:
| Veröffentlicht in: | FEMS microbiology letters 1992-12, Vol.100 (1-3), p.67-70 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 70 |
|---|---|
| container_issue | 1-3 |
| container_start_page | 67 |
| container_title | FEMS microbiology letters |
| container_volume | 100 |
| creator | LAMBERT, P. A KRIKLER, S. J RAGINI PATEL SAUDA PARVATHAN |
| description | An enzyme-linked immunosorbent assay (ELISA) was developed for measurement of the antibody response to exocellular protein antigens of Staphylococcus aureus. The wells of a microtitre plate were coated with the exocellular proteins present in the tryptic soya broth growth medium from a stationary phase culture of a bone infection strain of S. aureus. The wells were then reacted sequentially with patient sera, protein A-peroxidase conjugate and chromogenic substrate. Serum from patients with S. aureus bone infection gave a significantly higher IgG titre than sera from patients with Staphylococcus epidermidis or Streptococcus sanguis bone infection or healthy uninfected individuals. The assay therefore appears to have potential in the serodiagnosis of S. aureus bone infection. |
| doi_str_mv | 10.1111/j.1574-6968.1992.tb05683.x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_73444665</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>73444665</sourcerecordid><originalsourceid>FETCH-LOGICAL-c218t-23a4c97a55bd2d4c82ef8ecf311c0929d661ecfce6cdbb7679ca0fc00e9da25e3</originalsourceid><addsrcrecordid>eNpFUcFq3DAQFaEh3aT9hIAopTc7kixLVm8lpE0gkEPbs5ClMdHWlraSDLu59sdrs0syl8fovRkN7yH0iZKaLnWzrWkreSWU6GqqFKtLT1rRNfX-DG1eqXdoQxrZVZQo-R5d5rwlhHBGxAW6oFx2vGMb9O8uvBwmqEYf_oDDfprmEHNMPYSCTc7mgOOAyzNgBwVs8TGsDyYU30fnIeMSMeyjhXGcR5PwLsUCPuRV9bOY3fNhjDZaO2ds5gQL-ID7GGDB4bjwAzofzJjh4wmv0O_vd79u76vHpx8Pt98eK8toVyrWGG6VNG3bO-a47RgMHdihodQSxZQTgi6tBWFd30shlTVksISAcoa10FyhL8e9y41_Z8hFTz6vh5sAcc5aNpxzIdpF-PUotCnmnGDQu-Qnkw6aEr0moLd6tVmvNus1AX1KQO-X4evTL3M_gXsbPVq-8J9PvMnWjEMywfr8KuOtbCRrm_-Qp5UW</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>73444665</pqid></control><display><type>article</type><title>Enzyme-linked immunosorbent assay of the detection of antibodies to exocellular proteins of Staphylococcus aureus in bone infection</title><source>MEDLINE</source><source>Oxford University Press Journals Digital Archive Legacy</source><source>Access via Wiley Online Library</source><source>Alma/SFX Local Collection</source><creator>LAMBERT, P. A ; KRIKLER, S. J ; RAGINI PATEL ; SAUDA PARVATHAN</creator><creatorcontrib>LAMBERT, P. A ; KRIKLER, S. J ; RAGINI PATEL ; SAUDA PARVATHAN</creatorcontrib><description>An enzyme-linked immunosorbent assay (ELISA) was developed for measurement of the antibody response to exocellular protein antigens of Staphylococcus aureus. The wells of a microtitre plate were coated with the exocellular proteins present in the tryptic soya broth growth medium from a stationary phase culture of a bone infection strain of S. aureus. The wells were then reacted sequentially with patient sera, protein A-peroxidase conjugate and chromogenic substrate. Serum from patients with S. aureus bone infection gave a significantly higher IgG titre than sera from patients with Staphylococcus epidermidis or Streptococcus sanguis bone infection or healthy uninfected individuals. The assay therefore appears to have potential in the serodiagnosis of S. aureus bone infection.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1111/j.1574-6968.1992.tb05683.x</identifier><identifier>PMID: 1478482</identifier><identifier>CODEN: FMLED7</identifier><language>eng</language><publisher>Oxford: Blackwell</publisher><subject>Antibodies, Bacterial - blood ; Antigens, Bacterial ; Bacterial Proteins - immunology ; Bacteriological methods and techniques used in bacteriology ; Bacteriology ; Biological and medical sciences ; Enzyme-Linked Immunosorbent Assay - methods ; Evaluation Studies as Topic ; Fundamental and applied biological sciences. Psychology ; Humans ; Immunoglobulin G - blood ; Microbiology ; Osteomyelitis - diagnosis ; Osteomyelitis - immunology ; Staphylococcal Infections - diagnosis ; Staphylococcal Infections - immunology ; Staphylococcus aureus - immunology</subject><ispartof>FEMS microbiology letters, 1992-12, Vol.100 (1-3), p.67-70</ispartof><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c218t-23a4c97a55bd2d4c82ef8ecf311c0929d661ecfce6cdbb7679ca0fc00e9da25e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4573725$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1478482$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>LAMBERT, P. A</creatorcontrib><creatorcontrib>KRIKLER, S. J</creatorcontrib><creatorcontrib>RAGINI PATEL</creatorcontrib><creatorcontrib>SAUDA PARVATHAN</creatorcontrib><title>Enzyme-linked immunosorbent assay of the detection of antibodies to exocellular proteins of Staphylococcus aureus in bone infection</title><title>FEMS microbiology letters</title><addtitle>FEMS Microbiol Lett</addtitle><description>An enzyme-linked immunosorbent assay (ELISA) was developed for measurement of the antibody response to exocellular protein antigens of Staphylococcus aureus. The wells of a microtitre plate were coated with the exocellular proteins present in the tryptic soya broth growth medium from a stationary phase culture of a bone infection strain of S. aureus. The wells were then reacted sequentially with patient sera, protein A-peroxidase conjugate and chromogenic substrate. Serum from patients with S. aureus bone infection gave a significantly higher IgG titre than sera from patients with Staphylococcus epidermidis or Streptococcus sanguis bone infection or healthy uninfected individuals. The assay therefore appears to have potential in the serodiagnosis of S. aureus bone infection.</description><subject>Antibodies, Bacterial - blood</subject><subject>Antigens, Bacterial</subject><subject>Bacterial Proteins - immunology</subject><subject>Bacteriological methods and techniques used in bacteriology</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Evaluation Studies as Topic</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Immunoglobulin G - blood</subject><subject>Microbiology</subject><subject>Osteomyelitis - diagnosis</subject><subject>Osteomyelitis - immunology</subject><subject>Staphylococcal Infections - diagnosis</subject><subject>Staphylococcal Infections - immunology</subject><subject>Staphylococcus aureus - immunology</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFUcFq3DAQFaEh3aT9hIAopTc7kixLVm8lpE0gkEPbs5ClMdHWlraSDLu59sdrs0syl8fovRkN7yH0iZKaLnWzrWkreSWU6GqqFKtLT1rRNfX-DG1eqXdoQxrZVZQo-R5d5rwlhHBGxAW6oFx2vGMb9O8uvBwmqEYf_oDDfprmEHNMPYSCTc7mgOOAyzNgBwVs8TGsDyYU30fnIeMSMeyjhXGcR5PwLsUCPuRV9bOY3fNhjDZaO2ds5gQL-ID7GGDB4bjwAzofzJjh4wmv0O_vd79u76vHpx8Pt98eK8toVyrWGG6VNG3bO-a47RgMHdihodQSxZQTgi6tBWFd30shlTVksISAcoa10FyhL8e9y41_Z8hFTz6vh5sAcc5aNpxzIdpF-PUotCnmnGDQu-Qnkw6aEr0moLd6tVmvNus1AX1KQO-X4evTL3M_gXsbPVq-8J9PvMnWjEMywfr8KuOtbCRrm_-Qp5UW</recordid><startdate>19921215</startdate><enddate>19921215</enddate><creator>LAMBERT, P. A</creator><creator>KRIKLER, S. J</creator><creator>RAGINI PATEL</creator><creator>SAUDA PARVATHAN</creator><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19921215</creationdate><title>Enzyme-linked immunosorbent assay of the detection of antibodies to exocellular proteins of Staphylococcus aureus in bone infection</title><author>LAMBERT, P. A ; KRIKLER, S. J ; RAGINI PATEL ; SAUDA PARVATHAN</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c218t-23a4c97a55bd2d4c82ef8ecf311c0929d661ecfce6cdbb7679ca0fc00e9da25e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Antibodies, Bacterial - blood</topic><topic>Antigens, Bacterial</topic><topic>Bacterial Proteins - immunology</topic><topic>Bacteriological methods and techniques used in bacteriology</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>Evaluation Studies as Topic</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Immunoglobulin G - blood</topic><topic>Microbiology</topic><topic>Osteomyelitis - diagnosis</topic><topic>Osteomyelitis - immunology</topic><topic>Staphylococcal Infections - diagnosis</topic><topic>Staphylococcal Infections - immunology</topic><topic>Staphylococcus aureus - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>LAMBERT, P. A</creatorcontrib><creatorcontrib>KRIKLER, S. J</creatorcontrib><creatorcontrib>RAGINI PATEL</creatorcontrib><creatorcontrib>SAUDA PARVATHAN</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>LAMBERT, P. A</au><au>KRIKLER, S. J</au><au>RAGINI PATEL</au><au>SAUDA PARVATHAN</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Enzyme-linked immunosorbent assay of the detection of antibodies to exocellular proteins of Staphylococcus aureus in bone infection</atitle><jtitle>FEMS microbiology letters</jtitle><addtitle>FEMS Microbiol Lett</addtitle><date>1992-12-15</date><risdate>1992</risdate><volume>100</volume><issue>1-3</issue><spage>67</spage><epage>70</epage><pages>67-70</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><coden>FMLED7</coden><abstract>An enzyme-linked immunosorbent assay (ELISA) was developed for measurement of the antibody response to exocellular protein antigens of Staphylococcus aureus. The wells of a microtitre plate were coated with the exocellular proteins present in the tryptic soya broth growth medium from a stationary phase culture of a bone infection strain of S. aureus. The wells were then reacted sequentially with patient sera, protein A-peroxidase conjugate and chromogenic substrate. Serum from patients with S. aureus bone infection gave a significantly higher IgG titre than sera from patients with Staphylococcus epidermidis or Streptococcus sanguis bone infection or healthy uninfected individuals. The assay therefore appears to have potential in the serodiagnosis of S. aureus bone infection.</abstract><cop>Oxford</cop><pub>Blackwell</pub><pmid>1478482</pmid><doi>10.1111/j.1574-6968.1992.tb05683.x</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1097 |
| ispartof | FEMS microbiology letters, 1992-12, Vol.100 (1-3), p.67-70 |
| issn | 0378-1097 1574-6968 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_73444665 |
| source | MEDLINE; Oxford University Press Journals Digital Archive Legacy; Access via Wiley Online Library; Alma/SFX Local Collection |
| subjects | Antibodies, Bacterial - blood Antigens, Bacterial Bacterial Proteins - immunology Bacteriological methods and techniques used in bacteriology Bacteriology Biological and medical sciences Enzyme-Linked Immunosorbent Assay - methods Evaluation Studies as Topic Fundamental and applied biological sciences. Psychology Humans Immunoglobulin G - blood Microbiology Osteomyelitis - diagnosis Osteomyelitis - immunology Staphylococcal Infections - diagnosis Staphylococcal Infections - immunology Staphylococcus aureus - immunology |
| title | Enzyme-linked immunosorbent assay of the detection of antibodies to exocellular proteins of Staphylococcus aureus in bone infection |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-23T18%3A20%3A23IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Enzyme-linked%20immunosorbent%20assay%20of%20the%20detection%20of%20antibodies%20to%20exocellular%20proteins%20of%20Staphylococcus%20aureus%20in%20bone%20infection&rft.jtitle=FEMS%20microbiology%20letters&rft.au=LAMBERT,%20P.%20A&rft.date=1992-12-15&rft.volume=100&rft.issue=1-3&rft.spage=67&rft.epage=70&rft.pages=67-70&rft.issn=0378-1097&rft.eissn=1574-6968&rft.coden=FMLED7&rft_id=info:doi/10.1111/j.1574-6968.1992.tb05683.x&rft_dat=%3Cproquest_cross%3E73444665%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=73444665&rft_id=info:pmid/1478482&rfr_iscdi=true |