Influence of extracellular bicarbonate on the short-circuit current and intracellular free calcium of human cultured sweat duct cells
Transepithelial short-circuit current (Iscc) and intracellular free Ca2+ (Ca2+i) was studied in monolayers of cultured human sweat duct cells (CSDCs) in the presence or absence of HCO3- (and CO2) in the bathing solutions. Addition of HCO3- (and CO2) increased the control Iscc by more than 50%. The e...
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| Veröffentlicht in: | Experimental physiology 1992-11, Vol.77 (6), p.863-871 |
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| description | Transepithelial short-circuit current (Iscc) and intracellular free Ca2+ (Ca2+i) was studied in monolayers of cultured human
sweat duct cells (CSDCs) in the presence or absence of HCO3- (and CO2) in the bathing solutions. Addition of HCO3- (and CO2)
increased the control Iscc by more than 50%. The effect of HCO3- (and CO2) on Iscc was confined to the serosal bath. The HCO3-
(and CO2) effect was also studied during stimulation with the cholinergic agonist methacholine (MCh), which in CSDC induces
a complex response consisting of an initial Iscc and Ca2+i spike, which is independent of extracellular Ca2+, followed by
regular Iscc and Ca2+i oscillations, which are absent during Ca(2+)-free bathing conditions. The sustained Iscc and Ca2+i
oscillations, but not the initial Iscc and Ca2+i spike were abolished by the removal of extracellular HCO3- (and CO2). It
is concluded that the Ca2+ influx and the Iscc in CSDCs are critically influenced by the presence of extracellular HCO3- (and
CO2) in the bathing solutions. |
| doi_str_mv | 10.1113/expphysiol.1992.sp003653 |
| format | Article |
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sweat duct cells (CSDCs) in the presence or absence of HCO3- (and CO2) in the bathing solutions. Addition of HCO3- (and CO2)
increased the control Iscc by more than 50%. The effect of HCO3- (and CO2) on Iscc was confined to the serosal bath. The HCO3-
(and CO2) effect was also studied during stimulation with the cholinergic agonist methacholine (MCh), which in CSDC induces
a complex response consisting of an initial Iscc and Ca2+i spike, which is independent of extracellular Ca2+, followed by
regular Iscc and Ca2+i oscillations, which are absent during Ca(2+)-free bathing conditions. The sustained Iscc and Ca2+i
oscillations, but not the initial Iscc and Ca2+i spike were abolished by the removal of extracellular HCO3- (and CO2). It
is concluded that the Ca2+ influx and the Iscc in CSDCs are critically influenced by the presence of extracellular HCO3- (and
CO2) in the bathing solutions.</description><identifier>ISSN: 0958-0670</identifier><identifier>EISSN: 1469-445X</identifier><identifier>DOI: 10.1113/expphysiol.1992.sp003653</identifier><identifier>PMID: 1489544</identifier><language>eng</language><publisher>Cambridge: The Physiological Society</publisher><subject>Amiloride - pharmacology ; Bicarbonates - metabolism ; Bicarbonates - pharmacology ; Biological and medical sciences ; Buffers ; Calcium - physiology ; Cells, Cultured ; Electrophysiology ; Extracellular Space - metabolism ; Fundamental and applied biological sciences. Psychology ; Humans ; Intracellular Membranes - metabolism ; Methacholine Chloride - pharmacology ; Sweat Glands - cytology ; Sweat Glands - metabolism ; Vertebrates: skin, associated glands, phaneres, light organs, various exocrine glands (salt gland, uropygial gland...), adipose tissue, connective tissue</subject><ispartof>Experimental physiology, 1992-11, Vol.77 (6), p.863-871</ispartof><rights>1992 The Physiological Society</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5133-87461f6e0eb83e7b33b7f42347cab2bac853da738b97137a17271a6e8faed11c3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1113%2Fexpphysiol.1992.sp003653$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1113%2Fexpphysiol.1992.sp003653$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4486917$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1489544$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pedersen, PS</creatorcontrib><title>Influence of extracellular bicarbonate on the short-circuit current and intracellular free calcium of human cultured sweat duct cells</title><title>Experimental physiology</title><addtitle>Exp Physiol</addtitle><description>Transepithelial short-circuit current (Iscc) and intracellular free Ca2+ (Ca2+i) was studied in monolayers of cultured human
sweat duct cells (CSDCs) in the presence or absence of HCO3- (and CO2) in the bathing solutions. Addition of HCO3- (and CO2)
increased the control Iscc by more than 50%. The effect of HCO3- (and CO2) on Iscc was confined to the serosal bath. The HCO3-
(and CO2) effect was also studied during stimulation with the cholinergic agonist methacholine (MCh), which in CSDC induces
a complex response consisting of an initial Iscc and Ca2+i spike, which is independent of extracellular Ca2+, followed by
regular Iscc and Ca2+i oscillations, which are absent during Ca(2+)-free bathing conditions. The sustained Iscc and Ca2+i
oscillations, but not the initial Iscc and Ca2+i spike were abolished by the removal of extracellular HCO3- (and CO2). It
is concluded that the Ca2+ influx and the Iscc in CSDCs are critically influenced by the presence of extracellular HCO3- (and
CO2) in the bathing solutions.</description><subject>Amiloride - pharmacology</subject><subject>Bicarbonates - metabolism</subject><subject>Bicarbonates - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Buffers</subject><subject>Calcium - physiology</subject><subject>Cells, Cultured</subject><subject>Electrophysiology</subject><subject>Extracellular Space - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Intracellular Membranes - metabolism</subject><subject>Methacholine Chloride - pharmacology</subject><subject>Sweat Glands - cytology</subject><subject>Sweat Glands - metabolism</subject><subject>Vertebrates: skin, associated glands, phaneres, light organs, various exocrine glands (salt gland, uropygial gland...), adipose tissue, connective tissue</subject><issn>0958-0670</issn><issn>1469-445X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc2KFDEUhYMoYzv6CEIWIm6qTTqpJLWUZnQGBnSh4C6kUresSFWqzQ89_QC-tymqtV2Jq0DOd8693IMQpmRLKWVv4eFwGE7RzeOWNs1uGw-EMFGzR2hDuWgqzuuvj9GGNLWqiJDkKXoW43dCKCOKX6ErylVTc75BP-98P2bwFvDcY3hIwVgYxzyagFtnTWhnb1IRPU4D4DjMIVXWBZtdwjaHAD5h4zvs_N_WPgBga0br8rQED3kyvvBjygE6HI9gEu6yLRnFEp-jJ70ZI7w4v9foy_ubz_vb6v7jh7v9u_vK1pSxSkkuaC-AQKsYyJaxVvZ8x7i0pt21xqqadUYy1TaSMmmo3ElqBKjeQEepZdfo9Zp7CPOPDDHpycVlA-NhzlFLxjknjBfwzT9BKgThhBa2oGpFbZhjDNDrQ3CTCSdNiV7K0pey9FKW_l1Wsb48T8ntBN3FuLZT9Fdn3cRyzD4Yb138g3GuRENlwfYrdnQjnP57vL75dLt8SCmUYJfbDO7bcHQB9OqKs3WQTlpKLfQC_gKx5scD</recordid><startdate>19921101</startdate><enddate>19921101</enddate><creator>Pedersen, PS</creator><general>The Physiological Society</general><general>Cambridge University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7X8</scope></search><sort><creationdate>19921101</creationdate><title>Influence of extracellular bicarbonate on the short-circuit current and intracellular free calcium of human cultured sweat duct cells</title><author>Pedersen, PS</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5133-87461f6e0eb83e7b33b7f42347cab2bac853da738b97137a17271a6e8faed11c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Amiloride - pharmacology</topic><topic>Bicarbonates - metabolism</topic><topic>Bicarbonates - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Buffers</topic><topic>Calcium - physiology</topic><topic>Cells, Cultured</topic><topic>Electrophysiology</topic><topic>Extracellular Space - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Intracellular Membranes - metabolism</topic><topic>Methacholine Chloride - pharmacology</topic><topic>Sweat Glands - cytology</topic><topic>Sweat Glands - metabolism</topic><topic>Vertebrates: skin, associated glands, phaneres, light organs, various exocrine glands (salt gland, uropygial gland...), adipose tissue, connective tissue</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pedersen, PS</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Experimental physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pedersen, PS</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Influence of extracellular bicarbonate on the short-circuit current and intracellular free calcium of human cultured sweat duct cells</atitle><jtitle>Experimental physiology</jtitle><addtitle>Exp Physiol</addtitle><date>1992-11-01</date><risdate>1992</risdate><volume>77</volume><issue>6</issue><spage>863</spage><epage>871</epage><pages>863-871</pages><issn>0958-0670</issn><eissn>1469-445X</eissn><abstract>Transepithelial short-circuit current (Iscc) and intracellular free Ca2+ (Ca2+i) was studied in monolayers of cultured human
sweat duct cells (CSDCs) in the presence or absence of HCO3- (and CO2) in the bathing solutions. Addition of HCO3- (and CO2)
increased the control Iscc by more than 50%. The effect of HCO3- (and CO2) on Iscc was confined to the serosal bath. The HCO3-
(and CO2) effect was also studied during stimulation with the cholinergic agonist methacholine (MCh), which in CSDC induces
a complex response consisting of an initial Iscc and Ca2+i spike, which is independent of extracellular Ca2+, followed by
regular Iscc and Ca2+i oscillations, which are absent during Ca(2+)-free bathing conditions. The sustained Iscc and Ca2+i
oscillations, but not the initial Iscc and Ca2+i spike were abolished by the removal of extracellular HCO3- (and CO2). It
is concluded that the Ca2+ influx and the Iscc in CSDCs are critically influenced by the presence of extracellular HCO3- (and
CO2) in the bathing solutions.</abstract><cop>Cambridge</cop><pub>The Physiological Society</pub><pmid>1489544</pmid><doi>10.1113/expphysiol.1992.sp003653</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Experimental physiology, 1992-11, Vol.77 (6), p.863-871 |
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| language | eng |
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| source | MEDLINE; Wiley Online Library All Journals; Alma/SFX Local Collection |
| subjects | Amiloride - pharmacology Bicarbonates - metabolism Bicarbonates - pharmacology Biological and medical sciences Buffers Calcium - physiology Cells, Cultured Electrophysiology Extracellular Space - metabolism Fundamental and applied biological sciences. Psychology Humans Intracellular Membranes - metabolism Methacholine Chloride - pharmacology Sweat Glands - cytology Sweat Glands - metabolism Vertebrates: skin, associated glands, phaneres, light organs, various exocrine glands (salt gland, uropygial gland...), adipose tissue, connective tissue |
| title | Influence of extracellular bicarbonate on the short-circuit current and intracellular free calcium of human cultured sweat duct cells |
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