A Colorimetric Microassay for the Detection of Agents that Interact with DNA

A simple microtiter assay for the detection of compounds that bind DNA is described. Agents that displace methyl green from DNA are detected spectrophotometrically by a decrease in absorbance at 630 nm. The feasibility of using the assay for detecting DNA-active compounds in fermentation extracts wa...

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Veröffentlicht in:	Journal of natural products (Washington, D.C.) D.C.), 1992-11, Vol.55 (11), p.1582-1587
Hauptverfasser:	Burres, Neal S, Frigo, Andrea, Rasmussen, Ronald R, McAlpine, James B
Format:	Artikel
Sprache:	eng
Schlagworte:	Aminoglycosides Anti-Bacterial Agents - pharmacology Antibiotics, Antineoplastic - pharmacology Colorimetry DNA - analysis DNA - drug effects Ethidium Fermentation Methyl Green Spectrometry, Fluorescence
Online-Zugang:	Volltext
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