A Colorimetric Microassay for the Detection of Agents that Interact with DNA

A Colorimetric Microassay for the Detection of Agents that Interact with DNA

A simple microtiter assay for the detection of compounds that bind DNA is described. Agents that displace methyl green from DNA are detected spectrophotometrically by a decrease in absorbance at 630 nm. The feasibility of using the assay for detecting DNA-active compounds in fermentation extracts wa...

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Veröffentlicht in:Journal of natural products (Washington, D.C.) D.C.), 1992-11, Vol.55 (11), p.1582-1587
Hauptverfasser: Burres, Neal S, Frigo, Andrea, Rasmussen, Ronald R, McAlpine, James B
Format: Artikel
Sprache:eng
Schlagworte:
Aminoglycosides
Anti-Bacterial Agents - pharmacology
Antibiotics, Antineoplastic - pharmacology
Colorimetry
DNA - analysis
DNA - drug effects
Ethidium
Fermentation
Methyl Green
Spectrometry, Fluorescence
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Zusammenfassung:A simple microtiter assay for the detection of compounds that bind DNA is described. Agents that displace methyl green from DNA are detected spectrophotometrically by a decrease in absorbance at 630 nm. The feasibility of using the assay for detecting DNA-active compounds in fermentation extracts was assessed, and the activities of reference compounds in the methyl green assay and an ethidium bromide displacement method were compared.
ISSN:0163-3864
1520-6025
DOI:10.1021/np50089a004