Comparative purification of recombinant HIV-1 and HIV-2 reverse transcriptase: Preparation of heterodimeric enzyme devoid of unprocessed gene product

A procedure'for producing and purifying recombinant HIV-1 and HIV-2 reverse transcriptase (RT) is described. These enzymes are produced by Escherichia coli-transformed with a plasmid containing the gene encoding for either the human immunodeficiency virus type 1 (HIV-1) or HIV-2 RT protein. Bot...

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Veröffentlicht in:Protein expression and purification 1992-12, Vol.3 (6), p.479-487
Hauptverfasser: Warren, Thomas C., Miglietta, John J., Shrutkowski, Anthony, Rose, Janice M., Rogers, Sheri L., Lubbej, Klaus, Shih, Cheng K., Caviness, Gary O., Ingraham, Richard, Palladino, Deborah E.H., David, Eva, Chow, Grace C., Kopp, Elizabeth B., Cohen, Kenneth A., Glinski, Jan A., Farina, Peter R., Grob, Peter M.
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Sprache:eng
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