Characterization of HPV-16 E6/E7 transcription in CaSki cells by quantitative PCR
Human papillomavirus (HPV) is associated with specific benign and malignant lesions of the epithelial and mucosal surfaces. Of the sexually transmitted types, HPV type 16 (HPV-16) is the most commonly associated with carcinoma of the uterine cervix. Expression of the E6/E7 open reading frame of the...
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| Veröffentlicht in: | Molecular and cellular probes 1992-12, Vol.6 (6), p.459-466 |
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| creator | HSU, E. M MCNICOL, P. J |
| description | Human papillomavirus (HPV) is associated with specific benign and malignant lesions of the epithelial and mucosal surfaces. Of the sexually transmitted types, HPV type 16 (HPV-16) is the most commonly associated with carcinoma of the uterine cervix. Expression of the E6/E7 open reading frame of the viral genome is considered critical in the development of neoplasia. Using the CaSki cervical carcinoma cell line as a model system, we have adapted the polymerase chain reaction to quantify the transcripts expressed from this region. It was found that 97.1% of the total spliced transcript is E6*I, which putatively encodes the E7 oncoprotein, while E6*II comprises 2.9% of spliced product. The ratio of E6*I to E6*II expression may be an important parameter in evaluating the disease risk associated with HPV-16 infection. |
| doi_str_mv | 10.1016/0890-8508(92)90042-V |
| format | Article |
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The ratio of E6*I to E6*II expression may be an important parameter in evaluating the disease risk associated with HPV-16 infection.</description><identifier>ISSN: 0890-8508</identifier><identifier>EISSN: 1096-1194</identifier><identifier>DOI: 10.1016/0890-8508(92)90042-V</identifier><identifier>PMID: 1480186</identifier><identifier>CODEN: MCPRE6</identifier><language>eng</language><publisher>Kidlington: Elsevier</publisher><subject>Base Sequence ; Biological and medical sciences ; Carcinoma - microbiology ; Carcinoma - pathology ; DNA Probes, HPV ; Female ; Female genital diseases ; Gene Expression Regulation, Neoplastic ; Gene Expression Regulation, Viral ; Gynecology. Andrology. Obstetrics ; Humans ; Medical sciences ; Molecular Sequence Data ; Oncogene Proteins, Viral - biosynthesis ; Oncogene Proteins, Viral - genetics ; Papillomavirus E7 Proteins ; Polymerase Chain Reaction ; Repressor Proteins ; RNA Splicing ; RNA, Neoplasm - analysis ; RNA, Viral - analysis ; Transcription, Genetic ; Tumor Cells, Cultured - metabolism ; Tumor Cells, Cultured - microbiology ; Tumors ; Uterine Cervical Neoplasms - microbiology ; Uterine Cervical Neoplasms - pathology</subject><ispartof>Molecular and cellular probes, 1992-12, Vol.6 (6), p.459-466</ispartof><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4498182$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1480186$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>HSU, E. M</creatorcontrib><creatorcontrib>MCNICOL, P. J</creatorcontrib><title>Characterization of HPV-16 E6/E7 transcription in CaSki cells by quantitative PCR</title><title>Molecular and cellular probes</title><addtitle>Mol Cell Probes</addtitle><description>Human papillomavirus (HPV) is associated with specific benign and malignant lesions of the epithelial and mucosal surfaces. Of the sexually transmitted types, HPV type 16 (HPV-16) is the most commonly associated with carcinoma of the uterine cervix. Expression of the E6/E7 open reading frame of the viral genome is considered critical in the development of neoplasia. Using the CaSki cervical carcinoma cell line as a model system, we have adapted the polymerase chain reaction to quantify the transcripts expressed from this region. It was found that 97.1% of the total spliced transcript is E6*I, which putatively encodes the E7 oncoprotein, while E6*II comprises 2.9% of spliced product. The ratio of E6*I to E6*II expression may be an important parameter in evaluating the disease risk associated with HPV-16 infection.</description><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Carcinoma - microbiology</subject><subject>Carcinoma - pathology</subject><subject>DNA Probes, HPV</subject><subject>Female</subject><subject>Female genital diseases</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Gene Expression Regulation, Viral</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Molecular Sequence Data</subject><subject>Oncogene Proteins, Viral - biosynthesis</subject><subject>Oncogene Proteins, Viral - genetics</subject><subject>Papillomavirus E7 Proteins</subject><subject>Polymerase Chain Reaction</subject><subject>Repressor Proteins</subject><subject>RNA Splicing</subject><subject>RNA, Neoplasm - analysis</subject><subject>RNA, Viral - analysis</subject><subject>Transcription, Genetic</subject><subject>Tumor Cells, Cultured - metabolism</subject><subject>Tumor Cells, Cultured - microbiology</subject><subject>Tumors</subject><subject>Uterine Cervical Neoplasms - microbiology</subject><subject>Uterine Cervical Neoplasms - pathology</subject><issn>0890-8508</issn><issn>1096-1194</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kM1OwzAQhC0EKqXwBiD5gBAcQnfjxLWPKAoUqRLlr9fIcRxhSJM0TpDK05NC1L3sYb5dzQwh5wi3CMinICR4IgRxLf0bCRD43uqAjBEk9xBlcEjGe-SYnDj3CQAyADEiIwwEoOBj8hx9qEbp1jT2R7W2KmmV0_ly5SGnMZ_GM9o2qnS6sfWfaksaqdcvS7UpCkfTLd10qmxt2x9_G7qMXk7JUa4KZ86GPSHv9_FbNPcWTw-P0d3Cq30Wtp5JNeNaolCYZZpzVBkP051hxNCAFGkfyeQsx5RBhj4P0TfSgEbj92PYhFz9_62batMZ1yZr63auVGmqziUzFjAOOOvBiwHs0rXJkrqxa9Vsk6GDXr8cdOW0KvI-r7ZujwWBFCh89gtrq2lS</recordid><startdate>19921201</startdate><enddate>19921201</enddate><creator>HSU, E. 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J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p235t-ebc36c918a1ddc661ad65b0890115e098b900ef3f1b30d126512e9e0c1e2222e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Carcinoma - microbiology</topic><topic>Carcinoma - pathology</topic><topic>DNA Probes, HPV</topic><topic>Female</topic><topic>Female genital diseases</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Gene Expression Regulation, Viral</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Molecular Sequence Data</topic><topic>Oncogene Proteins, Viral - biosynthesis</topic><topic>Oncogene Proteins, Viral - genetics</topic><topic>Papillomavirus E7 Proteins</topic><topic>Polymerase Chain Reaction</topic><topic>Repressor Proteins</topic><topic>RNA Splicing</topic><topic>RNA, Neoplasm - analysis</topic><topic>RNA, Viral - analysis</topic><topic>Transcription, Genetic</topic><topic>Tumor Cells, Cultured - metabolism</topic><topic>Tumor Cells, Cultured - microbiology</topic><topic>Tumors</topic><topic>Uterine Cervical Neoplasms - microbiology</topic><topic>Uterine Cervical Neoplasms - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>HSU, E. M</creatorcontrib><creatorcontrib>MCNICOL, P. 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Using the CaSki cervical carcinoma cell line as a model system, we have adapted the polymerase chain reaction to quantify the transcripts expressed from this region. It was found that 97.1% of the total spliced transcript is E6*I, which putatively encodes the E7 oncoprotein, while E6*II comprises 2.9% of spliced product. The ratio of E6*I to E6*II expression may be an important parameter in evaluating the disease risk associated with HPV-16 infection.</abstract><cop>Kidlington</cop><pub>Elsevier</pub><pmid>1480186</pmid><doi>10.1016/0890-8508(92)90042-V</doi><tpages>8</tpages></addata></record> |
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| ispartof | Molecular and cellular probes, 1992-12, Vol.6 (6), p.459-466 |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Base Sequence Biological and medical sciences Carcinoma - microbiology Carcinoma - pathology DNA Probes, HPV Female Female genital diseases Gene Expression Regulation, Neoplastic Gene Expression Regulation, Viral Gynecology. Andrology. Obstetrics Humans Medical sciences Molecular Sequence Data Oncogene Proteins, Viral - biosynthesis Oncogene Proteins, Viral - genetics Papillomavirus E7 Proteins Polymerase Chain Reaction Repressor Proteins RNA Splicing RNA, Neoplasm - analysis RNA, Viral - analysis Transcription, Genetic Tumor Cells, Cultured - metabolism Tumor Cells, Cultured - microbiology Tumors Uterine Cervical Neoplasms - microbiology Uterine Cervical Neoplasms - pathology |
| title | Characterization of HPV-16 E6/E7 transcription in CaSki cells by quantitative PCR |
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