Enteral glutamine stimulates protein synthesis and decreases ubiquitin mRNA level in human gut mucosa
Effects of glutamine on whole body and intestinal protein synthesis and on intestinal proteolysis were assessed in humans. Two groups of healthy volunteers received in a random order enteral glutamine (0.8 mmol.kg body wt(-1)x h(-1)) compared either to saline or isonitrogenous amino acids. Intraveno...
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| Veröffentlicht in: | American journal of physiology: Gastrointestinal and liver physiology 2003-08, Vol.285 (2), p.G266-G273 |
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| container_title | American journal of physiology: Gastrointestinal and liver physiology |
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| creator | Coëffier, Moïse Claeyssens, Sophie Hecketsweiler, Bernadette Lavoinne, Alain Ducrotté, Philippe Déchelotte, Pierre |
| description | Effects of glutamine on whole body and intestinal protein synthesis and on intestinal proteolysis were assessed in humans. Two groups of healthy volunteers received in a random order enteral glutamine (0.8 mmol.kg body wt(-1)x h(-1)) compared either to saline or isonitrogenous amino acids. Intravenous [2H5]phenylalanine and [13C]leucine were simultaneously infused. After gas chromatography-mass spectrometry analysis, whole body protein turnover was estimated from traced plasma amino acid fluxes and the fractional synthesis rate (FSR) of gut mucosal protein was calculated from protein and intracellular phenylalanine and leucine enrichments in duodenal biopsies. mRNA levels for ubiquitin, cathepsin D, and m-calpain were analyzed in biopsies by RT-PCR. Glutamine significantly increased mucosal protein FSR compared with saline. Glutamine and amino acids had similar effects on FSR. The mRNA level for ubiquitin was significantly decreased after glutamine infusion compared with saline and amino acids, whereas cathepsin D and m-calpain mRNA levels were not affected. Enteral glutamine stimulates mucosal protein synthesis and may attenuate ubiquitin-dependent proteolysis and thus improve protein balance in human gut. |
| doi_str_mv | 10.1152/ajpgi.00385.2002 |
| format | Article |
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Two groups of healthy volunteers received in a random order enteral glutamine (0.8 mmol.kg body wt(-1)x h(-1)) compared either to saline or isonitrogenous amino acids. Intravenous [2H5]phenylalanine and [13C]leucine were simultaneously infused. After gas chromatography-mass spectrometry analysis, whole body protein turnover was estimated from traced plasma amino acid fluxes and the fractional synthesis rate (FSR) of gut mucosal protein was calculated from protein and intracellular phenylalanine and leucine enrichments in duodenal biopsies. mRNA levels for ubiquitin, cathepsin D, and m-calpain were analyzed in biopsies by RT-PCR. Glutamine significantly increased mucosal protein FSR compared with saline. Glutamine and amino acids had similar effects on FSR. The mRNA level for ubiquitin was significantly decreased after glutamine infusion compared with saline and amino acids, whereas cathepsin D and m-calpain mRNA levels were not affected. Enteral glutamine stimulates mucosal protein synthesis and may attenuate ubiquitin-dependent proteolysis and thus improve protein balance in human gut.</description><subject>Adult</subject><subject>Amino Acids - blood</subject><subject>Calpain - genetics</subject><subject>Carbon Isotopes</subject><subject>Cathepsin D - genetics</subject><subject>Deuterium</subject><subject>Duodenum - chemistry</subject><subject>Enteral Nutrition</subject><subject>Female</subject><subject>Gas Chromatography-Mass Spectrometry</subject><subject>Glutamine - administration & dosage</subject><subject>Humans</subject><subject>Insulin - blood</subject><subject>Intestinal Mucosa - chemistry</subject><subject>Intestinal Mucosa - drug effects</subject><subject>Intestinal Mucosa - metabolism</subject><subject>Intubation, Gastrointestinal</subject><subject>Leucine - administration & dosage</subject><subject>Leucine - analysis</subject><subject>Male</subject><subject>Phenylalanine - administration & dosage</subject><subject>Phenylalanine - analysis</subject><subject>Protein Biosynthesis</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - analysis</subject><subject>Ubiquitin - genetics</subject><issn>0193-1857</issn><issn>1522-1547</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkM1LAzEQxYMotlbvniQnb1snyaa7eyzFLygKoueQZGfblP1oN4nQ_97tB3gaZua9x-NHyD2DKWOSP-nNduWmACKXUw7AL8h4OPOEyTS7JGNghUhYLrMRufF-AwCSM3ZNRoxnwNNiNib43AbsdU1XdQy6cS1SH1wTax3Q023fBXQt9fs2rNE7T3Vb0hJtj9oP_2jcLrowKJqvjzmt8RdrOmzr2OiWrmKgTbSd17fkqtK1x7vznJCfl-fvxVuy_Hx9X8yXieWFDAkTUlTG5lZbM5TNmTYghQVWyVJkZTUrMl0Zzq1Jy9RmeYGARQpmVgldAivFhDyecofiu4g-qMZ5i3WtW-yiV5lIhSyAD0I4CW3fed9jpba9a3S_VwzUAa06olVHtOqAdrA8nLOjabD8N5xZij_8Mne6</recordid><startdate>200308</startdate><enddate>200308</enddate><creator>Coëffier, Moïse</creator><creator>Claeyssens, Sophie</creator><creator>Hecketsweiler, Bernadette</creator><creator>Lavoinne, Alain</creator><creator>Ducrotté, Philippe</creator><creator>Déchelotte, Pierre</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200308</creationdate><title>Enteral glutamine stimulates protein synthesis and decreases ubiquitin mRNA level in human gut mucosa</title><author>Coëffier, Moïse ; 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Two groups of healthy volunteers received in a random order enteral glutamine (0.8 mmol.kg body wt(-1)x h(-1)) compared either to saline or isonitrogenous amino acids. Intravenous [2H5]phenylalanine and [13C]leucine were simultaneously infused. After gas chromatography-mass spectrometry analysis, whole body protein turnover was estimated from traced plasma amino acid fluxes and the fractional synthesis rate (FSR) of gut mucosal protein was calculated from protein and intracellular phenylalanine and leucine enrichments in duodenal biopsies. mRNA levels for ubiquitin, cathepsin D, and m-calpain were analyzed in biopsies by RT-PCR. Glutamine significantly increased mucosal protein FSR compared with saline. Glutamine and amino acids had similar effects on FSR. The mRNA level for ubiquitin was significantly decreased after glutamine infusion compared with saline and amino acids, whereas cathepsin D and m-calpain mRNA levels were not affected. 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| source | MEDLINE; American Physiological Society; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Adult Amino Acids - blood Calpain - genetics Carbon Isotopes Cathepsin D - genetics Deuterium Duodenum - chemistry Enteral Nutrition Female Gas Chromatography-Mass Spectrometry Glutamine - administration & dosage Humans Insulin - blood Intestinal Mucosa - chemistry Intestinal Mucosa - drug effects Intestinal Mucosa - metabolism Intubation, Gastrointestinal Leucine - administration & dosage Leucine - analysis Male Phenylalanine - administration & dosage Phenylalanine - analysis Protein Biosynthesis Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - analysis Ubiquitin - genetics |
| title | Enteral glutamine stimulates protein synthesis and decreases ubiquitin mRNA level in human gut mucosa |
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