Reversible changes in protein phosphorylation during germinal vesicle breakdown and pronuclear formation in bovine oocytes in vitro
This study examined the event of protein phosphorylation in bovine oocytes during germinal vesicle breakdown (GVBD) and formation of pronuclei following fertilisation in vitro. Immature oocytes were obtained from abattoir materials and cultured in vitro. The oocytes were labelled with [32P]orthophos...
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| Veröffentlicht in: | Zygote (Cambridge) 2003-05, Vol.11 (2), p.119-129 |
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| creator | Chian, R.C. Chung, J.T. Niwa, K. Sirard, M.A. Downey, B.R. Tan, S.L. |
| description | This study examined the event of protein phosphorylation in bovine oocytes during germinal vesicle breakdown (GVBD) and formation of pronuclei following fertilisation in vitro. Immature oocytes were obtained from abattoir materials and cultured in vitro. The oocytes were labelled with [32P]orthophosphate at 3 h intervals from 0 to 12 h following maturation in culture or from 3 to 18 h following insemination. One-dimensional gel electrophoresis indicated that levels of protein phosphorylation are low prior to GVBD. However, the levels of protein phosphorylation at approximately 40 kDa, 27 kDa, 23 kDa and 18 kDa increased substantially following GVBD and then decreased gradually as maturation in culture progressed. In contrast, the levels of protein phosphorylation increased gradually in the oocytes following pronucleus formation. Further, two-dimensional gel electrophoresis indicated that the protein at approximately 18 kDa reversibly changed in the oocytes during maturation and fertilisation. These results indicate that the reversible changes of this phosphoprotein may be related to either cell cycle transition or pronucleus formation during maturation and fertilisation in bovine oocytes. |
| doi_str_mv | 10.1017/S0967199403002156 |
| format | Article |
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Immature oocytes were obtained from abattoir materials and cultured in vitro. The oocytes were labelled with [32P]orthophosphate at 3 h intervals from 0 to 12 h following maturation in culture or from 3 to 18 h following insemination. One-dimensional gel electrophoresis indicated that levels of protein phosphorylation are low prior to GVBD. However, the levels of protein phosphorylation at approximately 40 kDa, 27 kDa, 23 kDa and 18 kDa increased substantially following GVBD and then decreased gradually as maturation in culture progressed. In contrast, the levels of protein phosphorylation increased gradually in the oocytes following pronucleus formation. Further, two-dimensional gel electrophoresis indicated that the protein at approximately 18 kDa reversibly changed in the oocytes during maturation and fertilisation. These results indicate that the reversible changes of this phosphoprotein may be related to either cell cycle transition or pronucleus formation during maturation and fertilisation in bovine oocytes.</description><identifier>ISSN: 0967-1994</identifier><identifier>EISSN: 1469-8730</identifier><identifier>DOI: 10.1017/S0967199403002156</identifier><identifier>PMID: 12828411</identifier><language>eng</language><publisher>Cambridge, UK: Cambridge University Press</publisher><subject>Animals ; Cattle ; Cell Nucleus - metabolism ; Female ; GVBD ; Male ; Maturation ; Oocyte ; Oocytes - physiology ; Phosphorylation ; Phosphotransferases - metabolism ; Pronucleus ; Protein phosphorylation ; Proteins - metabolism ; Time Factors ; Zygote - physiology</subject><ispartof>Zygote (Cambridge), 2003-05, Vol.11 (2), p.119-129</ispartof><rights>2003 Cambridge University Press</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c493t-7509072e65817c08928a9c48c96bb1b06e92363b4d7ad408971bcc1e6e6ef9823</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.cambridge.org/core/product/identifier/S0967199403002156/type/journal_article$$EHTML$$P50$$Gcambridge$$H</linktohtml><link.rule.ids>164,314,776,780,27901,27902,55603</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12828411$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chian, R.C.</creatorcontrib><creatorcontrib>Chung, J.T.</creatorcontrib><creatorcontrib>Niwa, K.</creatorcontrib><creatorcontrib>Sirard, M.A.</creatorcontrib><creatorcontrib>Downey, B.R.</creatorcontrib><creatorcontrib>Tan, S.L.</creatorcontrib><title>Reversible changes in protein phosphorylation during germinal vesicle breakdown and pronuclear formation in bovine oocytes in vitro</title><title>Zygote (Cambridge)</title><addtitle>Zygote</addtitle><description>This study examined the event of protein phosphorylation in bovine oocytes during germinal vesicle breakdown (GVBD) and formation of pronuclei following fertilisation in vitro. 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These results indicate that the reversible changes of this phosphoprotein may be related to either cell cycle transition or pronucleus formation during maturation and fertilisation in bovine oocytes.</description><subject>Animals</subject><subject>Cattle</subject><subject>Cell Nucleus - metabolism</subject><subject>Female</subject><subject>GVBD</subject><subject>Male</subject><subject>Maturation</subject><subject>Oocyte</subject><subject>Oocytes - physiology</subject><subject>Phosphorylation</subject><subject>Phosphotransferases - metabolism</subject><subject>Pronucleus</subject><subject>Protein phosphorylation</subject><subject>Proteins - metabolism</subject><subject>Time Factors</subject><subject>Zygote - physiology</subject><issn>0967-1994</issn><issn>1469-8730</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp1kV9vFCEUxYnR2LX6AXwxxAffRmFgYXg0m9rWtPG_8Y0Ac3dLOwMVZrbuc794mcxGG40h5Cac37kcuAg9p-Q1JVS--UKUkFQpThghNV2KB2hBuVBVIxl5iBaTXE36AXqS8yUhRErFH6MDWjd1wyldoNvPsIWUve0AuwsTNpCxD_g6xQGmehFz2WnXmcHHgNsx-bDBG0i9D6bDW8jeFatNYK7aeBOwCe3kDmM5NgmvY-pna-lm49YHwDG63TDfs_VDik_Ro7XpMjzb10P07d3R19VJdfbh-HT19qxyXLGhkkuiiKxBLBsqHWlU3RjleOOUsJZaIkDVTDDLW2laXnRJrXMURFlr1dTsEL2a-5Z8P0fIg-59dtB1JkAcs5aMM6b4BL78C7yMYyrvzZoqwcp3M1kgOkMuxZwTrPV18r1JO02Jnsaj_xlP8bzYNx5tD-0fx34eBahmwOcBfv3WTbrSQjK51OL4k_7x_f0JWX0k-rzwbB_C9Db5dgP3ov43xh3H86r6</recordid><startdate>200305</startdate><enddate>200305</enddate><creator>Chian, R.C.</creator><creator>Chung, J.T.</creator><creator>Niwa, K.</creator><creator>Sirard, M.A.</creator><creator>Downey, B.R.</creator><creator>Tan, S.L.</creator><general>Cambridge University Press</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>200305</creationdate><title>Reversible changes in protein phosphorylation during germinal vesicle breakdown and pronuclear formation in bovine oocytes in vitro</title><author>Chian, R.C. ; Chung, J.T. ; Niwa, K. ; Sirard, M.A. ; Downey, B.R. ; Tan, S.L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c493t-7509072e65817c08928a9c48c96bb1b06e92363b4d7ad408971bcc1e6e6ef9823</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>Cattle</topic><topic>Cell Nucleus - metabolism</topic><topic>Female</topic><topic>GVBD</topic><topic>Male</topic><topic>Maturation</topic><topic>Oocyte</topic><topic>Oocytes - physiology</topic><topic>Phosphorylation</topic><topic>Phosphotransferases - metabolism</topic><topic>Pronucleus</topic><topic>Protein phosphorylation</topic><topic>Proteins - metabolism</topic><topic>Time Factors</topic><topic>Zygote - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chian, R.C.</creatorcontrib><creatorcontrib>Chung, J.T.</creatorcontrib><creatorcontrib>Niwa, K.</creatorcontrib><creatorcontrib>Sirard, M.A.</creatorcontrib><creatorcontrib>Downey, B.R.</creatorcontrib><creatorcontrib>Tan, S.L.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Zygote (Cambridge)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chian, R.C.</au><au>Chung, J.T.</au><au>Niwa, K.</au><au>Sirard, M.A.</au><au>Downey, B.R.</au><au>Tan, S.L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Reversible changes in protein phosphorylation during germinal vesicle breakdown and pronuclear formation in bovine oocytes in vitro</atitle><jtitle>Zygote (Cambridge)</jtitle><addtitle>Zygote</addtitle><date>2003-05</date><risdate>2003</risdate><volume>11</volume><issue>2</issue><spage>119</spage><epage>129</epage><pages>119-129</pages><issn>0967-1994</issn><eissn>1469-8730</eissn><abstract>This study examined the event of protein phosphorylation in bovine oocytes during germinal vesicle breakdown (GVBD) and formation of pronuclei following fertilisation in vitro. Immature oocytes were obtained from abattoir materials and cultured in vitro. The oocytes were labelled with [32P]orthophosphate at 3 h intervals from 0 to 12 h following maturation in culture or from 3 to 18 h following insemination. One-dimensional gel electrophoresis indicated that levels of protein phosphorylation are low prior to GVBD. However, the levels of protein phosphorylation at approximately 40 kDa, 27 kDa, 23 kDa and 18 kDa increased substantially following GVBD and then decreased gradually as maturation in culture progressed. In contrast, the levels of protein phosphorylation increased gradually in the oocytes following pronucleus formation. Further, two-dimensional gel electrophoresis indicated that the protein at approximately 18 kDa reversibly changed in the oocytes during maturation and fertilisation. These results indicate that the reversible changes of this phosphoprotein may be related to either cell cycle transition or pronucleus formation during maturation and fertilisation in bovine oocytes.</abstract><cop>Cambridge, UK</cop><pub>Cambridge University Press</pub><pmid>12828411</pmid><doi>10.1017/S0967199403002156</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Cattle Cell Nucleus - metabolism Female GVBD Male Maturation Oocyte Oocytes - physiology Phosphorylation Phosphotransferases - metabolism Pronucleus Protein phosphorylation Proteins - metabolism Time Factors Zygote - physiology |
| title | Reversible changes in protein phosphorylation during germinal vesicle breakdown and pronuclear formation in bovine oocytes in vitro |
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