Transfection of murine P19S18 embryonal carcinoma cells with the oncogene neu induces an epithelioid phenotype
. An embryonal carcinoma cell line, P19S18, was transfected with the rat oncogene neu to investigate the function of its protein product, p185*, in a multipotential cellular environment. Levels of message for p185* were determined by in situ hybridization analysis and two highly expressing clones, P...
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| Veröffentlicht in: | Differentiation (London) 1992-10, Vol.51 (2), p.129-135 |
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| creator | Kibbey, Maura C. Mazurkiewicz, Joseph E. |
| description | . An embryonal carcinoma cell line, P19S18, was transfected with the rat oncogene neu to investigate the function of its protein product, p185*, in a multipotential cellular environment. Levels of message for p185* were determined by in situ hybridization analysis and two highly expressing clones, PnnA and PnnB, were isolated. As demonstrated by indirect immunofluorescence and immunoprecipitation, these neu‐transfected cells synthesized a full length rat p185*. The transfectants do not resemble typical embryonal carcinoma cells either before or after differentiation is induced by retinoic acid treatment. They are much larger, flatter, “epithelioid” cells. These cells have lost the expression of stage specific embryonic antigen‐1 (SSEA‐1), but do synthesize and assemble the basement membrane components laminin and fibronectin. These results suggest that expression of the neu oncogene in a multipotential cell line may induce the synthesis of proteins indicative of an epithelioid phenotype due to the presence of p185*. |
| doi_str_mv | 10.1111/j.1432-0436.1992.tb00689.x |
| format | Article |
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An embryonal carcinoma cell line, P19S18, was transfected with the rat oncogene neu to investigate the function of its protein product, p185*, in a multipotential cellular environment. Levels of message for p185* were determined by in situ hybridization analysis and two highly expressing clones, PnnA and PnnB, were isolated. As demonstrated by indirect immunofluorescence and immunoprecipitation, these neu‐transfected cells synthesized a full length rat p185*. The transfectants do not resemble typical embryonal carcinoma cells either before or after differentiation is induced by retinoic acid treatment. They are much larger, flatter, “epithelioid” cells. These cells have lost the expression of stage specific embryonic antigen‐1 (SSEA‐1), but do synthesize and assemble the basement membrane components laminin and fibronectin. These results suggest that expression of the neu oncogene in a multipotential cell line may induce the synthesis of proteins indicative of an epithelioid phenotype due to the presence of p185*.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biomarkers, Tumor - physiology</subject><subject>Cell Differentiation - genetics</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Embryology: invertebrates and vertebrates. Teratology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Regulation</subject><subject>Immunohistochemistry</subject><subject>In Situ Hybridization</subject><subject>Mice</subject><subject>Molecular embryology</subject><subject>Neoplasm Transplantation</subject><subject>Neoplasms, Germ Cell and Embryonal - pathology</subject><subject>Proto-Oncogene Proteins - physiology</subject><subject>Receptor, ErbB-2</subject><subject>Transfection</subject><subject>Tumor Cells, Cultured</subject><issn>0301-4681</issn><issn>1432-0436</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkV2L1DAUhoMo6-zqTxCCiHetOUknTW4EWd11YUHBuQ9pPpwMbVKblt3596ZsWS_NTSDvc84J50HoPZAayvl0qqFhtCIN4zVISeu5I4QLWT--QLvn6CXaEUagariA1-gy5xMhRHAKF-gCGAcJYofiYdIxe2fmkCJOHg_LFKLDP0H-AoHd0E3nFHWPjZ5MiGnQ2Li-z_ghzEc8Hx1O0aTfrpREt-AQ7WJcxjpiNxbC9SEFi8eji2k-j-4NeuV1n93b7b5Ch5tvh-vv1f2P27vrL_fVyCjIisvWCt9Q0XrNHbfQNczT8miFta0zLaV72xkqgXnLm5Y1QFlnOfPGSu_ZFfr41Hac0p_F5VkNIa__1tGlJatSwKAl9L8g8LZgghfw3QYu3eCsGqcw6OmstkWW_MOW62x078tWTcjPWBnH9u2-YJ-fsIfQu_O_LkStYtVJrfbUak-tYtUmVj2qr3c3QCX7C2QimBU</recordid><startdate>199210</startdate><enddate>199210</enddate><creator>Kibbey, Maura C.</creator><creator>Mazurkiewicz, Joseph E.</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7TM</scope><scope>7TO</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>199210</creationdate><title>Transfection of murine P19S18 embryonal carcinoma cells with the oncogene neu induces an epithelioid phenotype</title><author>Kibbey, Maura C. ; Mazurkiewicz, Joseph E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p3219-697d8f4287fa6e6d1b43f297dd8dd7ec7225dbc2913fd64734123bd63fcd9ff3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biomarkers, Tumor - physiology</topic><topic>Cell Differentiation - genetics</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Embryology: invertebrates and vertebrates. Teratology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Regulation</topic><topic>Immunohistochemistry</topic><topic>In Situ Hybridization</topic><topic>Mice</topic><topic>Molecular embryology</topic><topic>Neoplasm Transplantation</topic><topic>Neoplasms, Germ Cell and Embryonal - pathology</topic><topic>Proto-Oncogene Proteins - physiology</topic><topic>Receptor, ErbB-2</topic><topic>Transfection</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kibbey, Maura C.</creatorcontrib><creatorcontrib>Mazurkiewicz, Joseph E.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Differentiation (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kibbey, Maura C.</au><au>Mazurkiewicz, Joseph E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transfection of murine P19S18 embryonal carcinoma cells with the oncogene neu induces an epithelioid phenotype</atitle><jtitle>Differentiation (London)</jtitle><addtitle>Differentiation</addtitle><date>1992-10</date><risdate>1992</risdate><volume>51</volume><issue>2</issue><spage>129</spage><epage>135</epage><pages>129-135</pages><issn>0301-4681</issn><eissn>1432-0436</eissn><abstract>. An embryonal carcinoma cell line, P19S18, was transfected with the rat oncogene neu to investigate the function of its protein product, p185*, in a multipotential cellular environment. Levels of message for p185* were determined by in situ hybridization analysis and two highly expressing clones, PnnA and PnnB, were isolated. As demonstrated by indirect immunofluorescence and immunoprecipitation, these neu‐transfected cells synthesized a full length rat p185*. The transfectants do not resemble typical embryonal carcinoma cells either before or after differentiation is induced by retinoic acid treatment. They are much larger, flatter, “epithelioid” cells. These cells have lost the expression of stage specific embryonic antigen‐1 (SSEA‐1), but do synthesize and assemble the basement membrane components laminin and fibronectin. These results suggest that expression of the neu oncogene in a multipotential cell line may induce the synthesis of proteins indicative of an epithelioid phenotype due to the presence of p185*.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>1361918</pmid><doi>10.1111/j.1432-0436.1992.tb00689.x</doi><tpages>7</tpages></addata></record> |
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| ispartof | Differentiation (London), 1992-10, Vol.51 (2), p.129-135 |
| issn | 0301-4681 1432-0436 |
| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Animals Biological and medical sciences Biomarkers, Tumor - physiology Cell Differentiation - genetics Electrophoresis, Polyacrylamide Gel Embryology: invertebrates and vertebrates. Teratology Fundamental and applied biological sciences. Psychology Gene Expression Regulation Immunohistochemistry In Situ Hybridization Mice Molecular embryology Neoplasm Transplantation Neoplasms, Germ Cell and Embryonal - pathology Proto-Oncogene Proteins - physiology Receptor, ErbB-2 Transfection Tumor Cells, Cultured |
| title | Transfection of murine P19S18 embryonal carcinoma cells with the oncogene neu induces an epithelioid phenotype |
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