Accelerated healing of skin burns by anti-Gal/α-gal liposomes interaction
Abstract Topical application of α-gal liposomes on burns results in rapid local recruitment of neutrophils and macrophages. Recruited macrophages are pivotal for healing of burns because they secrete cytokines/growth factors that induce epidermis regeneration and tissue repair. α-Gal liposomes have...
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| Veröffentlicht in: | Burns 2010-03, Vol.36 (2), p.239-251 |
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| creator | Galili, Uri Wigglesworth, Kim Abdel-Motal, Ussama M |
| description | Abstract Topical application of α-gal liposomes on burns results in rapid local recruitment of neutrophils and macrophages. Recruited macrophages are pivotal for healing of burns because they secrete cytokines/growth factors that induce epidermis regeneration and tissue repair. α-Gal liposomes have glycolipids with α-gal epitopes (Galα1-3Galβ1-4GlcNAc-R) which bind anti-Gal, the most abundant natural antibody in humans constituting ∼1% of immunoglobulins. Interaction of α-gal liposomes with anti-Gal within the fluid film formed on burns, activates complement and generates chemotactic complement cleavage peptides which effectively recruit neutrophils and macrophages. Anti-Gal IgG coating α-gal liposomes further binds to Fcγ receptors on macrophages and activates them to secrete cytokines/growth factors. Efficacy of α-gal liposomes treatment in accelerating burn healing is demonstrated in the experimental model of α1,3galactosyltransferase knockout mice. These mice are the only available nonprimate mammals that can produce anti-Gal in titers similar to those in humans. Pairs of burns in mice were covered either with a spot bandage coated with 10 mg α-gal liposomes, or with a control spot bandage coated with saline. On Day 3 post-treatment, the α-gal liposomes treated burns contained ∼5-fold as many neutrophils as control burns, whereas macrophages were found only in α-gal liposomes treated burns. On Day 6, 50–100% of the surface area of α-gal liposomes treated burns were covered with regenerating epidermis (re-epithelialization), whereas almost no epidermis was found in control burns. The extensive recruitment of macrophages by anti-Gal/α-gal liposomes interaction was further demonstrated in vivo with polyvinyl alcohol (PVA) sponge discs containing α-gal liposomes, implanted subcutaneously. Since anti-Gal is abundant in all humans, it is suggested that treatment with α-gal liposomes will be effective also in patients with burns and other skin wounds. |
| doi_str_mv | 10.1016/j.burns.2009.04.002 |
| format | Article |
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Recruited macrophages are pivotal for healing of burns because they secrete cytokines/growth factors that induce epidermis regeneration and tissue repair. α-Gal liposomes have glycolipids with α-gal epitopes (Galα1-3Galβ1-4GlcNAc-R) which bind anti-Gal, the most abundant natural antibody in humans constituting ∼1% of immunoglobulins. Interaction of α-gal liposomes with anti-Gal within the fluid film formed on burns, activates complement and generates chemotactic complement cleavage peptides which effectively recruit neutrophils and macrophages. Anti-Gal IgG coating α-gal liposomes further binds to Fcγ receptors on macrophages and activates them to secrete cytokines/growth factors. Efficacy of α-gal liposomes treatment in accelerating burn healing is demonstrated in the experimental model of α1,3galactosyltransferase knockout mice. These mice are the only available nonprimate mammals that can produce anti-Gal in titers similar to those in humans. Pairs of burns in mice were covered either with a spot bandage coated with 10 mg α-gal liposomes, or with a control spot bandage coated with saline. On Day 3 post-treatment, the α-gal liposomes treated burns contained ∼5-fold as many neutrophils as control burns, whereas macrophages were found only in α-gal liposomes treated burns. On Day 6, 50–100% of the surface area of α-gal liposomes treated burns were covered with regenerating epidermis (re-epithelialization), whereas almost no epidermis was found in control burns. The extensive recruitment of macrophages by anti-Gal/α-gal liposomes interaction was further demonstrated in vivo with polyvinyl alcohol (PVA) sponge discs containing α-gal liposomes, implanted subcutaneously. Since anti-Gal is abundant in all humans, it is suggested that treatment with α-gal liposomes will be effective also in patients with burns and other skin wounds.</description><identifier>ISSN: 0305-4179</identifier><identifier>EISSN: 1879-1409</identifier><identifier>DOI: 10.1016/j.burns.2009.04.002</identifier><identifier>PMID: 19501971</identifier><identifier>CODEN: BURND8</identifier><language>eng</language><publisher>Kidlington: Elsevier Ltd</publisher><subject>Animals ; Anti-Gal antibody ; Antigen-Antibody Reactions ; Bandages ; Biological and medical sciences ; Burns ; Burns - immunology ; Burns - pathology ; Burns - physiopathology ; Burns - therapy ; Chemotaxis ; Critical Care ; Epidermis - physiology ; Galactosyltransferases - immunology ; Immunization - methods ; Immunoglobulin G - immunology ; Liposomes ; Macrophages - pathology ; Medical sciences ; Mice ; Mice, Knockout ; Neutrophils - pathology ; Rabbits ; Regeneration ; Skin burn ; Traumas. Diseases due to physical agents ; Trisaccharides - administration & dosage ; Trisaccharides - immunology ; Wound Healing ; α-Gal glycolipids</subject><ispartof>Burns, 2010-03, Vol.36 (2), p.239-251</ispartof><rights>Elsevier Ltd and ISBI</rights><rights>2009 Elsevier Ltd and ISBI</rights><rights>2015 INIST-CNRS</rights><rights>Copyright (c) 2009 Elsevier Ltd and ISBI. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c443t-a53c3d9048e7532c52e8dfa2b2dd94bd2f4e7a714cba6d54ad4e22c5eff0320e3</citedby><cites>FETCH-LOGICAL-c443t-a53c3d9048e7532c52e8dfa2b2dd94bd2f4e7a714cba6d54ad4e22c5eff0320e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.burns.2009.04.002$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22500836$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19501971$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Galili, Uri</creatorcontrib><creatorcontrib>Wigglesworth, Kim</creatorcontrib><creatorcontrib>Abdel-Motal, Ussama M</creatorcontrib><title>Accelerated healing of skin burns by anti-Gal/α-gal liposomes interaction</title><title>Burns</title><addtitle>Burns</addtitle><description>Abstract Topical application of α-gal liposomes on burns results in rapid local recruitment of neutrophils and macrophages. Recruited macrophages are pivotal for healing of burns because they secrete cytokines/growth factors that induce epidermis regeneration and tissue repair. α-Gal liposomes have glycolipids with α-gal epitopes (Galα1-3Galβ1-4GlcNAc-R) which bind anti-Gal, the most abundant natural antibody in humans constituting ∼1% of immunoglobulins. Interaction of α-gal liposomes with anti-Gal within the fluid film formed on burns, activates complement and generates chemotactic complement cleavage peptides which effectively recruit neutrophils and macrophages. Anti-Gal IgG coating α-gal liposomes further binds to Fcγ receptors on macrophages and activates them to secrete cytokines/growth factors. Efficacy of α-gal liposomes treatment in accelerating burn healing is demonstrated in the experimental model of α1,3galactosyltransferase knockout mice. These mice are the only available nonprimate mammals that can produce anti-Gal in titers similar to those in humans. Pairs of burns in mice were covered either with a spot bandage coated with 10 mg α-gal liposomes, or with a control spot bandage coated with saline. On Day 3 post-treatment, the α-gal liposomes treated burns contained ∼5-fold as many neutrophils as control burns, whereas macrophages were found only in α-gal liposomes treated burns. On Day 6, 50–100% of the surface area of α-gal liposomes treated burns were covered with regenerating epidermis (re-epithelialization), whereas almost no epidermis was found in control burns. The extensive recruitment of macrophages by anti-Gal/α-gal liposomes interaction was further demonstrated in vivo with polyvinyl alcohol (PVA) sponge discs containing α-gal liposomes, implanted subcutaneously. Since anti-Gal is abundant in all humans, it is suggested that treatment with α-gal liposomes will be effective also in patients with burns and other skin wounds.</description><subject>Animals</subject><subject>Anti-Gal antibody</subject><subject>Antigen-Antibody Reactions</subject><subject>Bandages</subject><subject>Biological and medical sciences</subject><subject>Burns</subject><subject>Burns - immunology</subject><subject>Burns - pathology</subject><subject>Burns - physiopathology</subject><subject>Burns - therapy</subject><subject>Chemotaxis</subject><subject>Critical Care</subject><subject>Epidermis - physiology</subject><subject>Galactosyltransferases - immunology</subject><subject>Immunization - methods</subject><subject>Immunoglobulin G - immunology</subject><subject>Liposomes</subject><subject>Macrophages - pathology</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Knockout</subject><subject>Neutrophils - pathology</subject><subject>Rabbits</subject><subject>Regeneration</subject><subject>Skin burn</subject><subject>Traumas. Diseases due to physical agents</subject><subject>Trisaccharides - administration & dosage</subject><subject>Trisaccharides - immunology</subject><subject>Wound Healing</subject><subject>α-Gal glycolipids</subject><issn>0305-4179</issn><issn>1879-1409</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1u1DAURi1ERYfCEyChbBCrpNc_mcQLkKqqFKpKLIC15dg3xdOMPfhmkOaxeBGeqZ7OCCQ2eOPN-T77nsvYKw4NB748XzXDNkdqBIBuQDUA4glb8L7TNVegn7IFSGhrxTt9yp4TraCctodn7JTrFrju-ILdXDiHE2Y7o6--o51CvKvSWNF9iNVjfzXsKhvnUF_b6fz3r_rOTtUUNonSGqkKcS5hN4cUX7CT0U6EL4_3Gfv24err5cf69vP1p8uL29opJefattJJr0H12LVSuFZg70crBuG9VoMXo8LOdly5wS59q6xXKAqG4whSAMoz9vbQu8npxxZpNutAZYjJRkxbMp1UQot-KQopD6TLiSjjaDY5rG3eGQ5m79CszOOMZu_QgDLFYUm9PvZvhzX6v5mjtAK8OQKWnJ3GbKML9IcTogXo5bJw7w4cFhs_A2ZDLmB06ENGNxufwn8-8v6fvCvrCeXJe9whrVJJFNGGGxIGzJf9uvfbBg3AOdfyASn0plU</recordid><startdate>20100301</startdate><enddate>20100301</enddate><creator>Galili, Uri</creator><creator>Wigglesworth, Kim</creator><creator>Abdel-Motal, Ussama M</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20100301</creationdate><title>Accelerated healing of skin burns by anti-Gal/α-gal liposomes interaction</title><author>Galili, Uri ; Wigglesworth, Kim ; Abdel-Motal, Ussama M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c443t-a53c3d9048e7532c52e8dfa2b2dd94bd2f4e7a714cba6d54ad4e22c5eff0320e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Animals</topic><topic>Anti-Gal antibody</topic><topic>Antigen-Antibody Reactions</topic><topic>Bandages</topic><topic>Biological and medical sciences</topic><topic>Burns</topic><topic>Burns - immunology</topic><topic>Burns - pathology</topic><topic>Burns - physiopathology</topic><topic>Burns - therapy</topic><topic>Chemotaxis</topic><topic>Critical Care</topic><topic>Epidermis - physiology</topic><topic>Galactosyltransferases - immunology</topic><topic>Immunization - methods</topic><topic>Immunoglobulin G - immunology</topic><topic>Liposomes</topic><topic>Macrophages - pathology</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Knockout</topic><topic>Neutrophils - pathology</topic><topic>Rabbits</topic><topic>Regeneration</topic><topic>Skin burn</topic><topic>Traumas. Diseases due to physical agents</topic><topic>Trisaccharides - administration & dosage</topic><topic>Trisaccharides - immunology</topic><topic>Wound Healing</topic><topic>α-Gal glycolipids</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Galili, Uri</creatorcontrib><creatorcontrib>Wigglesworth, Kim</creatorcontrib><creatorcontrib>Abdel-Motal, Ussama M</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Burns</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Galili, Uri</au><au>Wigglesworth, Kim</au><au>Abdel-Motal, Ussama M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Accelerated healing of skin burns by anti-Gal/α-gal liposomes interaction</atitle><jtitle>Burns</jtitle><addtitle>Burns</addtitle><date>2010-03-01</date><risdate>2010</risdate><volume>36</volume><issue>2</issue><spage>239</spage><epage>251</epage><pages>239-251</pages><issn>0305-4179</issn><eissn>1879-1409</eissn><coden>BURND8</coden><abstract>Abstract Topical application of α-gal liposomes on burns results in rapid local recruitment of neutrophils and macrophages. Recruited macrophages are pivotal for healing of burns because they secrete cytokines/growth factors that induce epidermis regeneration and tissue repair. α-Gal liposomes have glycolipids with α-gal epitopes (Galα1-3Galβ1-4GlcNAc-R) which bind anti-Gal, the most abundant natural antibody in humans constituting ∼1% of immunoglobulins. Interaction of α-gal liposomes with anti-Gal within the fluid film formed on burns, activates complement and generates chemotactic complement cleavage peptides which effectively recruit neutrophils and macrophages. Anti-Gal IgG coating α-gal liposomes further binds to Fcγ receptors on macrophages and activates them to secrete cytokines/growth factors. Efficacy of α-gal liposomes treatment in accelerating burn healing is demonstrated in the experimental model of α1,3galactosyltransferase knockout mice. These mice are the only available nonprimate mammals that can produce anti-Gal in titers similar to those in humans. Pairs of burns in mice were covered either with a spot bandage coated with 10 mg α-gal liposomes, or with a control spot bandage coated with saline. On Day 3 post-treatment, the α-gal liposomes treated burns contained ∼5-fold as many neutrophils as control burns, whereas macrophages were found only in α-gal liposomes treated burns. On Day 6, 50–100% of the surface area of α-gal liposomes treated burns were covered with regenerating epidermis (re-epithelialization), whereas almost no epidermis was found in control burns. The extensive recruitment of macrophages by anti-Gal/α-gal liposomes interaction was further demonstrated in vivo with polyvinyl alcohol (PVA) sponge discs containing α-gal liposomes, implanted subcutaneously. Since anti-Gal is abundant in all humans, it is suggested that treatment with α-gal liposomes will be effective also in patients with burns and other skin wounds.</abstract><cop>Kidlington</cop><pub>Elsevier Ltd</pub><pmid>19501971</pmid><doi>10.1016/j.burns.2009.04.002</doi><tpages>13</tpages></addata></record> |
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| subjects | Animals Anti-Gal antibody Antigen-Antibody Reactions Bandages Biological and medical sciences Burns Burns - immunology Burns - pathology Burns - physiopathology Burns - therapy Chemotaxis Critical Care Epidermis - physiology Galactosyltransferases - immunology Immunization - methods Immunoglobulin G - immunology Liposomes Macrophages - pathology Medical sciences Mice Mice, Knockout Neutrophils - pathology Rabbits Regeneration Skin burn Traumas. Diseases due to physical agents Trisaccharides - administration & dosage Trisaccharides - immunology Wound Healing α-Gal glycolipids |
| title | Accelerated healing of skin burns by anti-Gal/α-gal liposomes interaction |
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