Fluorescence and photoacoustic spectroscopy of immobilized thylakoids
The O2 evolution activity of immobilized chloroplast membranes in different environments (albumin‐glutaraldehyde matrix, urethane polymer and alginate beads) is presented. As previously shown, the stability of photosystem II (PS II) of lettuce thylakoids appears to be increased by the immobilization...
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| Veröffentlicht in: | Biotechnology and bioengineering 1983-10, Vol.25 (10), p.2453-2468 |
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| container_end_page | 2468 |
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| container_issue | 10 |
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| container_title | Biotechnology and bioengineering |
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| creator | Thomasset, B Barbotin, J.N Thomas, D Thomasset, T Vejux, A |
| description | The O2 evolution activity of immobilized chloroplast membranes in different environments (albumin‐glutaraldehyde matrix, urethane polymer and alginate beads) is presented. As previously shown, the stability of photosystem II (PS II) of lettuce thylakoids appears to be increased by the immobilization process. For understanding such stability, some spectral investigations have been made about the energy distribution between the immobilized photosystems. The low‐temperature (77 K) fluorescence emission and photoacoustic spectroscopy are well adapted to solid particle studies. Especially, it has been shown that the fluorescence ratio (F735/F695) and photoacoustic ratio (PA676/PA440) are good indicators of the functional level of native and immobilized thylakoids. Such ratios are also given after storage and after continuous illumination conditions. Some results about the role played by glutaraldehyde (in the case of albumin‐glutar‐aldehyde matrix) in the stabilization process are also reported. |
| doi_str_mv | 10.1002/bit.260251014 |
| format | Article |
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As previously shown, the stability of photosystem II (PS II) of lettuce thylakoids appears to be increased by the immobilization process. For understanding such stability, some spectral investigations have been made about the energy distribution between the immobilized photosystems. The low‐temperature (77 K) fluorescence emission and photoacoustic spectroscopy are well adapted to solid particle studies. Especially, it has been shown that the fluorescence ratio (F735/F695) and photoacoustic ratio (PA676/PA440) are good indicators of the functional level of native and immobilized thylakoids. Such ratios are also given after storage and after continuous illumination conditions. 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Economical aspects ; Miscellaneous ; photosynthesis ; photosystem II ; plant biochemistry ; plant physiology ; spectroscopy ; thylakoid membranes ; thylakoids</subject><ispartof>Biotechnology and bioengineering, 1983-10, Vol.25 (10), p.2453-2468</ispartof><rights>Copyright © 1983 John Wiley & Sons, Inc.</rights><rights>1984 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4114-44fa5fa7405f2ae78e272d02e8735342706bfd8c9b6a0556a550f58a9f9e8eba3</citedby><cites>FETCH-LOGICAL-c4114-44fa5fa7405f2ae78e272d02e8735342706bfd8c9b6a0556a550f58a9f9e8eba3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fbit.260251014$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fbit.260251014$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=9319745$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18548573$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Thomasset, B</creatorcontrib><creatorcontrib>Barbotin, J.N</creatorcontrib><creatorcontrib>Thomas, D</creatorcontrib><creatorcontrib>Thomasset, T</creatorcontrib><creatorcontrib>Vejux, A</creatorcontrib><title>Fluorescence and photoacoustic spectroscopy of immobilized thylakoids</title><title>Biotechnology and bioengineering</title><addtitle>Biotechnol. Bioeng</addtitle><description>The O2 evolution activity of immobilized chloroplast membranes in different environments (albumin‐glutaraldehyde matrix, urethane polymer and alginate beads) is presented. As previously shown, the stability of photosystem II (PS II) of lettuce thylakoids appears to be increased by the immobilization process. For understanding such stability, some spectral investigations have been made about the energy distribution between the immobilized photosystems. The low‐temperature (77 K) fluorescence emission and photoacoustic spectroscopy are well adapted to solid particle studies. Especially, it has been shown that the fluorescence ratio (F735/F695) and photoacoustic ratio (PA676/PA440) are good indicators of the functional level of native and immobilized thylakoids. Such ratios are also given after storage and after continuous illumination conditions. Some results about the role played by glutaraldehyde (in the case of albumin‐glutar‐aldehyde matrix) in the stabilization process are also reported.</description><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Energy</subject><subject>fluorescence</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>immobilization</subject><subject>Industrial applications and implications. Economical aspects</subject><subject>Miscellaneous</subject><subject>photosynthesis</subject><subject>photosystem II</subject><subject>plant biochemistry</subject><subject>plant physiology</subject><subject>spectroscopy</subject><subject>thylakoid membranes</subject><subject>thylakoids</subject><issn>0006-3592</issn><issn>1097-0290</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1983</creationdate><recordtype>article</recordtype><recordid>eNp90L1v1DAYBnALgei1MLJChgqmlNdfsTPS0l6LqnZoKyQW641jU9PkHOKcyvHXY3TRwdTJivTL48cPIW8oHFEA9rEJ0xGrgEkKVDwjCwq1KoHV8JwsAKAquazZHtlP6Uf-VLqqXpI9qqXQUvEFOT3r1nF0ybqVdQWu2mK4j1NEG9dpCrZIg7PTGJONw6aIvgh9H5vQhd-uLab7TYcPMbTpFXnhsUvu9XwekLuz09uT8_Lyenlx8umytIJSUQrhUXpUAqRn6JR2TLEWmNOKSy6YgqrxrbZ1UyFIWaGU4KXG2tdOuwb5AfmwzR3G-HPt0mT6kKt3Ha5cLmxUDtEahMry_ZOScgWcKp5huYU2vzKNzpthDD2OG0PB_F3Y5IXNbuHs387B66Z37T89T5rB4QwwWez8iCsb0s7VnNZKyMzUlj2Gzm2evtQcX9z-32BuHNLkfu3-xPHBVIorab5eLc1SfKZfvqkrc579u633GA1-H3OZuxsGlAOrWM6U_A_RCavZ</recordid><startdate>198310</startdate><enddate>198310</enddate><creator>Thomasset, B</creator><creator>Barbotin, J.N</creator><creator>Thomas, D</creator><creator>Thomasset, T</creator><creator>Vejux, A</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>M7Z</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>198310</creationdate><title>Fluorescence and photoacoustic spectroscopy of immobilized thylakoids</title><author>Thomasset, B ; Barbotin, J.N ; Thomas, D ; Thomasset, T ; Vejux, A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4114-44fa5fa7405f2ae78e272d02e8735342706bfd8c9b6a0556a550f58a9f9e8eba3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1983</creationdate><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Energy</topic><topic>fluorescence</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>immobilization</topic><topic>Industrial applications and implications. Economical aspects</topic><topic>Miscellaneous</topic><topic>photosynthesis</topic><topic>photosystem II</topic><topic>plant biochemistry</topic><topic>plant physiology</topic><topic>spectroscopy</topic><topic>thylakoid membranes</topic><topic>thylakoids</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Thomasset, B</creatorcontrib><creatorcontrib>Barbotin, J.N</creatorcontrib><creatorcontrib>Thomas, D</creatorcontrib><creatorcontrib>Thomasset, T</creatorcontrib><creatorcontrib>Vejux, A</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biotechnology and bioengineering</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Thomasset, B</au><au>Barbotin, J.N</au><au>Thomas, D</au><au>Thomasset, T</au><au>Vejux, A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fluorescence and photoacoustic spectroscopy of immobilized thylakoids</atitle><jtitle>Biotechnology and bioengineering</jtitle><addtitle>Biotechnol. Bioeng</addtitle><date>1983-10</date><risdate>1983</risdate><volume>25</volume><issue>10</issue><spage>2453</spage><epage>2468</epage><pages>2453-2468</pages><issn>0006-3592</issn><eissn>1097-0290</eissn><coden>BIBIAU</coden><abstract>The O2 evolution activity of immobilized chloroplast membranes in different environments (albumin‐glutaraldehyde matrix, urethane polymer and alginate beads) is presented. As previously shown, the stability of photosystem II (PS II) of lettuce thylakoids appears to be increased by the immobilization process. For understanding such stability, some spectral investigations have been made about the energy distribution between the immobilized photosystems. The low‐temperature (77 K) fluorescence emission and photoacoustic spectroscopy are well adapted to solid particle studies. Especially, it has been shown that the fluorescence ratio (F735/F695) and photoacoustic ratio (PA676/PA440) are good indicators of the functional level of native and immobilized thylakoids. Such ratios are also given after storage and after continuous illumination conditions. Some results about the role played by glutaraldehyde (in the case of albumin‐glutar‐aldehyde matrix) in the stabilization process are also reported.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>18548573</pmid><doi>10.1002/bit.260251014</doi><tpages>16</tpages></addata></record> |
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| source | Wiley Online Library Journals Frontfile Complete |
| subjects | Biological and medical sciences Biotechnology Energy fluorescence Fundamental and applied biological sciences. Psychology immobilization Industrial applications and implications. Economical aspects Miscellaneous photosynthesis photosystem II plant biochemistry plant physiology spectroscopy thylakoid membranes thylakoids |
| title | Fluorescence and photoacoustic spectroscopy of immobilized thylakoids |
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