Early metaphase II oocytes treated with dibutyryl cyclic adenosine monophosphate provide suitable recipient cytoplasm for the production of miniature pig somatic cell nuclear transfer embryos

ABSTRACT We investigated the effects of in vitro maturation duration and treatment with dibutyryl cyclic adenosine monophosphate (dbcAMP) on the blind enucleation efficiency and developmental competence of miniature pig somatic cell nuclear transfer (SCNT) embryos. Oocytes were cultured for 22 h in...

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Veröffentlicht in:	Animal science journal 2010-02, Vol.81 (1), p.48-57
Hauptverfasser:	Sugimura, S., Tohoku Univ., Sendai (Japan), Yamanaka, K, Kawahara, M, Wakai, T, Yokoo, M, Sato, E
Format:	Artikel
Sprache:	eng
Schlagworte:	AMP Animal sciences Animals Blastocyst - drug effects Blastocyst - physiology Bucladesine - pharmacology Cell Nucleus - drug effects Cell Nucleus - physiology Cells, Cultured Cellular biology CERDO Chromosomes - drug effects Chromosomes - physiology CULTIVO IN VITRO CULTURE IN VITRO Embryology ENUCLEACION ENUCLEATION Female Hogs http://www.fao.org/aos/agrovoc#c_124 http://www.fao.org/aos/agrovoc#c_24135 http://www.fao.org/aos/agrovoc#c_2592 http://www.fao.org/aos/agrovoc#c_4655 http://www.fao.org/aos/agrovoc#c_5260 http://www.fao.org/aos/agrovoc#c_5454 http://www.fao.org/aos/agrovoc#c_7555 http://www.fao.org/aos/agrovoc#c_7778 http://www.fao.org/aos/agrovoc#c_7873 IN VITRO CULTURE MADURACION MATURATION Metaphase - drug effects Metaphase - physiology miniature pig Mitochondria - drug effects Mitochondria - physiology NOYAU CELLULAIRE nuclear transfer Nuclear Transfer Techniques NUCLEO NUCLEUS oocyte maturation Oocytes - cytology Oocytes - drug effects Oocytes - ultrastructure OVA OVULE OVULO PORCIN REPIQUAGE Reproductive technologies SWINE Swine, Miniature TEMPS TIEMPO TIME TRANSPLANTING TRASPLANTE
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