Early metaphase II oocytes treated with dibutyryl cyclic adenosine monophosphate provide suitable recipient cytoplasm for the production of miniature pig somatic cell nuclear transfer embryos
ABSTRACT We investigated the effects of in vitro maturation duration and treatment with dibutyryl cyclic adenosine monophosphate (dbcAMP) on the blind enucleation efficiency and developmental competence of miniature pig somatic cell nuclear transfer (SCNT) embryos. Oocytes were cultured for 22 h in...
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| Veröffentlicht in: | Animal science journal 2010-02, Vol.81 (1), p.48-57 |
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| creator | Sugimura, S., Tohoku Univ., Sendai (Japan) Yamanaka, K Kawahara, M Wakai, T Yokoo, M Sato, E |
| description | ABSTRACT
We investigated the effects of in vitro maturation duration and treatment with dibutyryl cyclic adenosine monophosphate (dbcAMP) on the blind enucleation efficiency and developmental competence of miniature pig somatic cell nuclear transfer (SCNT) embryos. Oocytes were cultured for 22 h in NCSU‐23 medium with or without 1 mM dbcAMP and then additionally cultured in dbcAMP‐free NCSU‐23 for 14, 18, or 22 h. Regardless of dbcAMP treatment, the rate of nuclear maturation reached a plateau at 36 and 40 h. However, mitochondrial distribution, a marker for cytoplasmic maturation, differed between the dbcAMP‐untreated oocytes at 36 h and dbcAMP‐treated oocytes at 40 h. The metaphase II chromosomes were adjacent to the first polar body in 68.8% and 63.5% of the dbcAMP‐untreated oocytes at 36 h and dbcAMP‐treated oocytes at 40 h, respectively. Furthermore, the blind enucleation efficiency by removing a small volume of cytoplasm was significantly higher in the dbcAMP‐untreated oocytes at 36 h (82.9%) and dbcAMP‐treated oocytes at 40 h (89.9%) than other groups. The rate of blastocyst formation was highest in the dbcAMP‐treated oocytes at 40 h. Hence, this study demonstrated that dbcAMP‐treated early metaphase II oocytes are suitable for the production of miniature pig SCNT embryos. |
| doi_str_mv | 10.1111/j.1740-0929.2009.00705.x |
| format | Article |
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We investigated the effects of in vitro maturation duration and treatment with dibutyryl cyclic adenosine monophosphate (dbcAMP) on the blind enucleation efficiency and developmental competence of miniature pig somatic cell nuclear transfer (SCNT) embryos. Oocytes were cultured for 22 h in NCSU‐23 medium with or without 1 mM dbcAMP and then additionally cultured in dbcAMP‐free NCSU‐23 for 14, 18, or 22 h. Regardless of dbcAMP treatment, the rate of nuclear maturation reached a plateau at 36 and 40 h. However, mitochondrial distribution, a marker for cytoplasmic maturation, differed between the dbcAMP‐untreated oocytes at 36 h and dbcAMP‐treated oocytes at 40 h. The metaphase II chromosomes were adjacent to the first polar body in 68.8% and 63.5% of the dbcAMP‐untreated oocytes at 36 h and dbcAMP‐treated oocytes at 40 h, respectively. Furthermore, the blind enucleation efficiency by removing a small volume of cytoplasm was significantly higher in the dbcAMP‐untreated oocytes at 36 h (82.9%) and dbcAMP‐treated oocytes at 40 h (89.9%) than other groups. The rate of blastocyst formation was highest in the dbcAMP‐treated oocytes at 40 h. Hence, this study demonstrated that dbcAMP‐treated early metaphase II oocytes are suitable for the production of miniature pig SCNT embryos.</description><identifier>ISSN: 1344-3941</identifier><identifier>EISSN: 1740-0929</identifier><identifier>DOI: 10.1111/j.1740-0929.2009.00705.x</identifier><identifier>PMID: 20163672</identifier><language>eng</language><publisher>Melbourne, Australia: Blackwell Publishing Asia</publisher><subject>AMP ; Animal sciences ; Animals ; Blastocyst - drug effects ; Blastocyst - physiology ; Bucladesine - pharmacology ; Cell Nucleus - drug effects ; Cell Nucleus - physiology ; Cells, Cultured ; Cellular biology ; CERDO ; Chromosomes - drug effects ; Chromosomes - physiology ; CULTIVO IN VITRO ; CULTURE IN VITRO ; Embryology ; ENUCLEACION ; ENUCLEATION ; Female ; Hogs ; http://www.fao.org/aos/agrovoc#c_124 ; http://www.fao.org/aos/agrovoc#c_24135 ; http://www.fao.org/aos/agrovoc#c_2592 ; http://www.fao.org/aos/agrovoc#c_4655 ; http://www.fao.org/aos/agrovoc#c_5260 ; http://www.fao.org/aos/agrovoc#c_5454 ; http://www.fao.org/aos/agrovoc#c_7555 ; http://www.fao.org/aos/agrovoc#c_7778 ; http://www.fao.org/aos/agrovoc#c_7873 ; IN VITRO CULTURE ; MADURACION ; MATURATION ; Metaphase - drug effects ; Metaphase - physiology ; miniature pig ; Mitochondria - drug effects ; Mitochondria - physiology ; NOYAU CELLULAIRE ; nuclear transfer ; Nuclear Transfer Techniques ; NUCLEO ; NUCLEUS ; oocyte maturation ; Oocytes - cytology ; Oocytes - drug effects ; Oocytes - ultrastructure ; OVA ; OVULE ; OVULO ; PORCIN ; REPIQUAGE ; Reproductive technologies ; SWINE ; Swine, Miniature ; TEMPS ; TIEMPO ; TIME ; TRANSPLANTING ; TRASPLANTE</subject><ispartof>Animal science journal, 2010-02, Vol.81 (1), p.48-57</ispartof><rights>2009 The Authors. Journal compilation © 2009 Japanese Society of Animal Science</rights><rights>Journal compilation © 2010 Japanese Society of Animal Science</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5075-734b3420f27ffdce0ab38c7110962bbeaf68bbc4a4574f1765bdc5019f3458973</citedby><cites>FETCH-LOGICAL-c5075-734b3420f27ffdce0ab38c7110962bbeaf68bbc4a4574f1765bdc5019f3458973</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1740-0929.2009.00705.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1740-0929.2009.00705.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20163672$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sugimura, S., Tohoku Univ., Sendai (Japan)</creatorcontrib><creatorcontrib>Yamanaka, K</creatorcontrib><creatorcontrib>Kawahara, M</creatorcontrib><creatorcontrib>Wakai, T</creatorcontrib><creatorcontrib>Yokoo, M</creatorcontrib><creatorcontrib>Sato, E</creatorcontrib><title>Early metaphase II oocytes treated with dibutyryl cyclic adenosine monophosphate provide suitable recipient cytoplasm for the production of miniature pig somatic cell nuclear transfer embryos</title><title>Animal science journal</title><addtitle>Anim Sci J</addtitle><description>ABSTRACT
We investigated the effects of in vitro maturation duration and treatment with dibutyryl cyclic adenosine monophosphate (dbcAMP) on the blind enucleation efficiency and developmental competence of miniature pig somatic cell nuclear transfer (SCNT) embryos. Oocytes were cultured for 22 h in NCSU‐23 medium with or without 1 mM dbcAMP and then additionally cultured in dbcAMP‐free NCSU‐23 for 14, 18, or 22 h. Regardless of dbcAMP treatment, the rate of nuclear maturation reached a plateau at 36 and 40 h. However, mitochondrial distribution, a marker for cytoplasmic maturation, differed between the dbcAMP‐untreated oocytes at 36 h and dbcAMP‐treated oocytes at 40 h. The metaphase II chromosomes were adjacent to the first polar body in 68.8% and 63.5% of the dbcAMP‐untreated oocytes at 36 h and dbcAMP‐treated oocytes at 40 h, respectively. Furthermore, the blind enucleation efficiency by removing a small volume of cytoplasm was significantly higher in the dbcAMP‐untreated oocytes at 36 h (82.9%) and dbcAMP‐treated oocytes at 40 h (89.9%) than other groups. The rate of blastocyst formation was highest in the dbcAMP‐treated oocytes at 40 h. Hence, this study demonstrated that dbcAMP‐treated early metaphase II oocytes are suitable for the production of miniature pig SCNT embryos.</description><subject>AMP</subject><subject>Animal sciences</subject><subject>Animals</subject><subject>Blastocyst - drug effects</subject><subject>Blastocyst - physiology</subject><subject>Bucladesine - pharmacology</subject><subject>Cell Nucleus - drug effects</subject><subject>Cell Nucleus - physiology</subject><subject>Cells, Cultured</subject><subject>Cellular biology</subject><subject>CERDO</subject><subject>Chromosomes - drug effects</subject><subject>Chromosomes - physiology</subject><subject>CULTIVO IN VITRO</subject><subject>CULTURE IN VITRO</subject><subject>Embryology</subject><subject>ENUCLEACION</subject><subject>ENUCLEATION</subject><subject>Female</subject><subject>Hogs</subject><subject>http://www.fao.org/aos/agrovoc#c_124</subject><subject>http://www.fao.org/aos/agrovoc#c_24135</subject><subject>http://www.fao.org/aos/agrovoc#c_2592</subject><subject>http://www.fao.org/aos/agrovoc#c_4655</subject><subject>http://www.fao.org/aos/agrovoc#c_5260</subject><subject>http://www.fao.org/aos/agrovoc#c_5454</subject><subject>http://www.fao.org/aos/agrovoc#c_7555</subject><subject>http://www.fao.org/aos/agrovoc#c_7778</subject><subject>http://www.fao.org/aos/agrovoc#c_7873</subject><subject>IN VITRO CULTURE</subject><subject>MADURACION</subject><subject>MATURATION</subject><subject>Metaphase - drug effects</subject><subject>Metaphase - physiology</subject><subject>miniature pig</subject><subject>Mitochondria - drug effects</subject><subject>Mitochondria - physiology</subject><subject>NOYAU CELLULAIRE</subject><subject>nuclear transfer</subject><subject>Nuclear Transfer Techniques</subject><subject>NUCLEO</subject><subject>NUCLEUS</subject><subject>oocyte maturation</subject><subject>Oocytes - cytology</subject><subject>Oocytes - drug effects</subject><subject>Oocytes - ultrastructure</subject><subject>OVA</subject><subject>OVULE</subject><subject>OVULO</subject><subject>PORCIN</subject><subject>REPIQUAGE</subject><subject>Reproductive technologies</subject><subject>SWINE</subject><subject>Swine, Miniature</subject><subject>TEMPS</subject><subject>TIEMPO</subject><subject>TIME</subject><subject>TRANSPLANTING</subject><subject>TRASPLANTE</subject><issn>1344-3941</issn><issn>1740-0929</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU-P1CAYxhujcdfVj6AhXjx1BEpLm3jZ3azjbCZq_BMTLwToyw5jWypQd_rp_GoyO-scPAkH3sDveXngyTJE8IKk8Xq7IJzhHDe0WVCMmwXGHJeL3YPs9HjwMNUFY3nRMHKSPQlhizHhDS4fZycUk6qoOD3Nfl9J382ohyjHjQyAVivknJ4jBBQ9yAgturVxg1qrpjj7uUN61p3VSLYwuGAHQL0b3LhxITWIgEbvftkWUJhslKoD5EHb0cIQkzK6sZOhR8Z5FDd3cDvpaN2AnEG9HayMk0_79gYF18uYLtLQdWiYdAcyibwcggGPoFd-duFp9sjILsCz-_Us-_r26svlu3z9Ybm6PF_nusS8zHnBVMEoNpQb02rAUhW15oTgpqJKgTRVrZRmkpWcGcKrUrVJSRpTsLJueHGWvTr0TY5_ThCi6G3YO5MDuCmIdAGtafrTRL78h9y6yQ_JnKCkrpqyrKsE1QdIexeCByNGb3vpZ0Gw2EcstmKfpNgnKfYRi7uIxS5JX9z3n1QP7VH4N9MEvDkAt7aD-b8bi_PP16lI8vwgtyHC7iiX_odIr-Ol-PZ-KViBl-sL8l18SvzzA2-kE_LG2yCuPyYzGGOaZvEHsFvRnA</recordid><startdate>201002</startdate><enddate>201002</enddate><creator>Sugimura, S., Tohoku Univ., Sendai (Japan)</creator><creator>Yamanaka, K</creator><creator>Kawahara, M</creator><creator>Wakai, T</creator><creator>Yokoo, M</creator><creator>Sato, E</creator><general>Blackwell Publishing Asia</general><general>Blackwell Publishing Ltd</general><scope>FBQ</scope><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>201002</creationdate><title>Early metaphase II oocytes treated with dibutyryl cyclic adenosine monophosphate provide suitable recipient cytoplasm for the production of miniature pig somatic cell nuclear transfer embryos</title><author>Sugimura, S., Tohoku Univ., Sendai (Japan) ; Yamanaka, K ; Kawahara, M ; Wakai, T ; Yokoo, M ; Sato, E</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5075-734b3420f27ffdce0ab38c7110962bbeaf68bbc4a4574f1765bdc5019f3458973</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>AMP</topic><topic>Animal sciences</topic><topic>Animals</topic><topic>Blastocyst - drug effects</topic><topic>Blastocyst - physiology</topic><topic>Bucladesine - pharmacology</topic><topic>Cell Nucleus - drug effects</topic><topic>Cell Nucleus - physiology</topic><topic>Cells, Cultured</topic><topic>Cellular biology</topic><topic>CERDO</topic><topic>Chromosomes - drug effects</topic><topic>Chromosomes - physiology</topic><topic>CULTIVO IN VITRO</topic><topic>CULTURE IN VITRO</topic><topic>Embryology</topic><topic>ENUCLEACION</topic><topic>ENUCLEATION</topic><topic>Female</topic><topic>Hogs</topic><topic>http://www.fao.org/aos/agrovoc#c_124</topic><topic>http://www.fao.org/aos/agrovoc#c_24135</topic><topic>http://www.fao.org/aos/agrovoc#c_2592</topic><topic>http://www.fao.org/aos/agrovoc#c_4655</topic><topic>http://www.fao.org/aos/agrovoc#c_5260</topic><topic>http://www.fao.org/aos/agrovoc#c_5454</topic><topic>http://www.fao.org/aos/agrovoc#c_7555</topic><topic>http://www.fao.org/aos/agrovoc#c_7778</topic><topic>http://www.fao.org/aos/agrovoc#c_7873</topic><topic>IN VITRO CULTURE</topic><topic>MADURACION</topic><topic>MATURATION</topic><topic>Metaphase - drug effects</topic><topic>Metaphase - physiology</topic><topic>miniature pig</topic><topic>Mitochondria - drug effects</topic><topic>Mitochondria - physiology</topic><topic>NOYAU CELLULAIRE</topic><topic>nuclear transfer</topic><topic>Nuclear Transfer Techniques</topic><topic>NUCLEO</topic><topic>NUCLEUS</topic><topic>oocyte maturation</topic><topic>Oocytes - cytology</topic><topic>Oocytes - drug effects</topic><topic>Oocytes - ultrastructure</topic><topic>OVA</topic><topic>OVULE</topic><topic>OVULO</topic><topic>PORCIN</topic><topic>REPIQUAGE</topic><topic>Reproductive technologies</topic><topic>SWINE</topic><topic>Swine, Miniature</topic><topic>TEMPS</topic><topic>TIEMPO</topic><topic>TIME</topic><topic>TRANSPLANTING</topic><topic>TRASPLANTE</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sugimura, S., Tohoku Univ., Sendai (Japan)</creatorcontrib><creatorcontrib>Yamanaka, K</creatorcontrib><creatorcontrib>Kawahara, M</creatorcontrib><creatorcontrib>Wakai, T</creatorcontrib><creatorcontrib>Yokoo, M</creatorcontrib><creatorcontrib>Sato, E</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Animal science journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sugimura, S., Tohoku Univ., Sendai (Japan)</au><au>Yamanaka, K</au><au>Kawahara, M</au><au>Wakai, T</au><au>Yokoo, M</au><au>Sato, E</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Early metaphase II oocytes treated with dibutyryl cyclic adenosine monophosphate provide suitable recipient cytoplasm for the production of miniature pig somatic cell nuclear transfer embryos</atitle><jtitle>Animal science journal</jtitle><addtitle>Anim Sci J</addtitle><date>2010-02</date><risdate>2010</risdate><volume>81</volume><issue>1</issue><spage>48</spage><epage>57</epage><pages>48-57</pages><issn>1344-3941</issn><eissn>1740-0929</eissn><abstract>ABSTRACT
We investigated the effects of in vitro maturation duration and treatment with dibutyryl cyclic adenosine monophosphate (dbcAMP) on the blind enucleation efficiency and developmental competence of miniature pig somatic cell nuclear transfer (SCNT) embryos. Oocytes were cultured for 22 h in NCSU‐23 medium with or without 1 mM dbcAMP and then additionally cultured in dbcAMP‐free NCSU‐23 for 14, 18, or 22 h. Regardless of dbcAMP treatment, the rate of nuclear maturation reached a plateau at 36 and 40 h. However, mitochondrial distribution, a marker for cytoplasmic maturation, differed between the dbcAMP‐untreated oocytes at 36 h and dbcAMP‐treated oocytes at 40 h. The metaphase II chromosomes were adjacent to the first polar body in 68.8% and 63.5% of the dbcAMP‐untreated oocytes at 36 h and dbcAMP‐treated oocytes at 40 h, respectively. Furthermore, the blind enucleation efficiency by removing a small volume of cytoplasm was significantly higher in the dbcAMP‐untreated oocytes at 36 h (82.9%) and dbcAMP‐treated oocytes at 40 h (89.9%) than other groups. The rate of blastocyst formation was highest in the dbcAMP‐treated oocytes at 40 h. Hence, this study demonstrated that dbcAMP‐treated early metaphase II oocytes are suitable for the production of miniature pig SCNT embryos.</abstract><cop>Melbourne, Australia</cop><pub>Blackwell Publishing Asia</pub><pmid>20163672</pmid><doi>10.1111/j.1740-0929.2009.00705.x</doi><tpages>10</tpages></addata></record> |
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| subjects | AMP Animal sciences Animals Blastocyst - drug effects Blastocyst - physiology Bucladesine - pharmacology Cell Nucleus - drug effects Cell Nucleus - physiology Cells, Cultured Cellular biology CERDO Chromosomes - drug effects Chromosomes - physiology CULTIVO IN VITRO CULTURE IN VITRO Embryology ENUCLEACION ENUCLEATION Female Hogs http://www.fao.org/aos/agrovoc#c_124 http://www.fao.org/aos/agrovoc#c_24135 http://www.fao.org/aos/agrovoc#c_2592 http://www.fao.org/aos/agrovoc#c_4655 http://www.fao.org/aos/agrovoc#c_5260 http://www.fao.org/aos/agrovoc#c_5454 http://www.fao.org/aos/agrovoc#c_7555 http://www.fao.org/aos/agrovoc#c_7778 http://www.fao.org/aos/agrovoc#c_7873 IN VITRO CULTURE MADURACION MATURATION Metaphase - drug effects Metaphase - physiology miniature pig Mitochondria - drug effects Mitochondria - physiology NOYAU CELLULAIRE nuclear transfer Nuclear Transfer Techniques NUCLEO NUCLEUS oocyte maturation Oocytes - cytology Oocytes - drug effects Oocytes - ultrastructure OVA OVULE OVULO PORCIN REPIQUAGE Reproductive technologies SWINE Swine, Miniature TEMPS TIEMPO TIME TRANSPLANTING TRASPLANTE |
| title | Early metaphase II oocytes treated with dibutyryl cyclic adenosine monophosphate provide suitable recipient cytoplasm for the production of miniature pig somatic cell nuclear transfer embryos |
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