Stable isotope fingerprinting: a novel method for identifying plant, fungal, or bacterial origins of amino acids
Amino acids play an important role in ecology as essential nutrients for animals and as currencies in symbiotic associations. Here we present a new approach to tracing the origins of amino acids by identifying unique patterns of carbon isotope signatures generated by amino acid synthesis in plants,...
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| Veröffentlicht in: | Ecology (Durham) 2009-12, Vol.90 (12), p.3526-3535 |
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| creator | Larsen, Thomas Taylor, D. Lee Leigh, Mary Beth O'Brien, Diane M |
| description | Amino acids play an important role in ecology as essential nutrients for animals and as currencies in symbiotic associations. Here we present a new approach to tracing the origins of amino acids by identifying unique patterns of carbon isotope signatures generated by amino acid synthesis in plants, fungi, and bacteria ("¹³C fingerprints"). We measured amino acid δ¹³C from 10 C₃ plants, 13 fungi, and 10 bacteria collected and isolated from a boreal forest in interior Alaska, USA, using gas chromatography-combustion-isotope ratio mass spectrometry (GC-C-IRMS). Microorganisms were cultured under amino-acid-free conditions and identified based on DNA sequences. Bacteria, fungi, and plants generated consistent, unique ¹³C fingerprints based on the more complex amino acids (five or more biosynthetic steps) that are classified as essential for animals. Linear discriminant analysis classified all samples correctly with >99% certainty and correctly classified nearly all insect samples from a previous study by diet. Our results suggest that ¹³C fingerprints of amino acids could provide a powerful in situ assay of the biosynthetic sources of amino acids and a potential new tool for understanding nutritional linkages in food webs. |
| doi_str_mv | 10.1890/08-1695.1 |
| format | Article |
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Lee ; Leigh, Mary Beth ; O'Brien, Diane M</creator><creatorcontrib>Larsen, Thomas ; Taylor, D. Lee ; Leigh, Mary Beth ; O'Brien, Diane M</creatorcontrib><description>Amino acids play an important role in ecology as essential nutrients for animals and as currencies in symbiotic associations. Here we present a new approach to tracing the origins of amino acids by identifying unique patterns of carbon isotope signatures generated by amino acid synthesis in plants, fungi, and bacteria ("¹³C fingerprints"). We measured amino acid δ¹³C from 10 C₃ plants, 13 fungi, and 10 bacteria collected and isolated from a boreal forest in interior Alaska, USA, using gas chromatography-combustion-isotope ratio mass spectrometry (GC-C-IRMS). Microorganisms were cultured under amino-acid-free conditions and identified based on DNA sequences. 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Our results suggest that ¹³C fingerprints of amino acids could provide a powerful in situ assay of the biosynthetic sources of amino acids and a potential new tool for understanding nutritional linkages in food webs.</description><identifier>ISSN: 0012-9658</identifier><identifier>EISSN: 1939-9170</identifier><identifier>DOI: 10.1890/08-1695.1</identifier><identifier>PMID: 20120819</identifier><identifier>CODEN: ECGYAQ</identifier><language>eng</language><publisher>Washington, DC: Ecological Society of America</publisher><subject>Alaska ; Amino acids ; Amino Acids - analysis ; Amino Acids - chemistry ; Animal and plant ecology ; Animal, plant and microbial ecology ; Bacteria ; Bacteria - chemistry ; Bacteria - classification ; Biological and medical sciences ; Biosynthesis ; Boreal forests ; carbon ; Carbon Isotopes - analysis ; Carbon Isotopes - chemistry ; Chromatography, High Pressure Liquid - methods ; compound-specific stable isotope analysis ; Discriminant Analysis ; Discriminants ; DNA ; DNA fingerprints ; Ecology ; Essential amino acids ; eukaryotes ; Flowers & plants ; food webs ; Fundamental and applied biological sciences. Psychology ; Fungi ; Fungi - chemistry ; Fungi - classification ; gas chromatography ; gas chromatography-combustion-isotope ratio mass spectrometry (GC-C-IRMS) ; General aspects ; Isotopes ; mass spectrometry ; nutrients ; Plants ; Plants - chemistry ; Plants - classification ; prokaryotes ; Species Specificity ; Spectrometry, Mass, Electrospray Ionization - methods ; stable isotopes ; Taxa ; trophic relationships ; USA ; δ13C</subject><ispartof>Ecology (Durham), 2009-12, Vol.90 (12), p.3526-3535</ispartof><rights>Copyright © 2009 Ecological Society of America</rights><rights>2009 by the Ecological Society of America</rights><rights>2015 INIST-CNRS</rights><rights>Copyright Ecological Society of America Dec 2009</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5666-9ff04c4643282417712c9865225cc52fd3919d7ca98d4d7436428900580114093</citedby><cites>FETCH-LOGICAL-c5666-9ff04c4643282417712c9865225cc52fd3919d7ca98d4d7436428900580114093</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/25660997$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/25660997$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,1416,27923,27924,45573,45574,58016,58249</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22254151$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20120819$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Larsen, Thomas</creatorcontrib><creatorcontrib>Taylor, D. Lee</creatorcontrib><creatorcontrib>Leigh, Mary Beth</creatorcontrib><creatorcontrib>O'Brien, Diane M</creatorcontrib><title>Stable isotope fingerprinting: a novel method for identifying plant, fungal, or bacterial origins of amino acids</title><title>Ecology (Durham)</title><addtitle>Ecology</addtitle><description>Amino acids play an important role in ecology as essential nutrients for animals and as currencies in symbiotic associations. Here we present a new approach to tracing the origins of amino acids by identifying unique patterns of carbon isotope signatures generated by amino acid synthesis in plants, fungi, and bacteria ("¹³C fingerprints"). We measured amino acid δ¹³C from 10 C₃ plants, 13 fungi, and 10 bacteria collected and isolated from a boreal forest in interior Alaska, USA, using gas chromatography-combustion-isotope ratio mass spectrometry (GC-C-IRMS). Microorganisms were cultured under amino-acid-free conditions and identified based on DNA sequences. Bacteria, fungi, and plants generated consistent, unique ¹³C fingerprints based on the more complex amino acids (five or more biosynthetic steps) that are classified as essential for animals. Linear discriminant analysis classified all samples correctly with >99% certainty and correctly classified nearly all insect samples from a previous study by diet. Our results suggest that ¹³C fingerprints of amino acids could provide a powerful in situ assay of the biosynthetic sources of amino acids and a potential new tool for understanding nutritional linkages in food webs.</description><subject>Alaska</subject><subject>Amino acids</subject><subject>Amino Acids - analysis</subject><subject>Amino Acids - chemistry</subject><subject>Animal and plant ecology</subject><subject>Animal, plant and microbial ecology</subject><subject>Bacteria</subject><subject>Bacteria - chemistry</subject><subject>Bacteria - classification</subject><subject>Biological and medical sciences</subject><subject>Biosynthesis</subject><subject>Boreal forests</subject><subject>carbon</subject><subject>Carbon Isotopes - analysis</subject><subject>Carbon Isotopes - chemistry</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>compound-specific stable isotope analysis</subject><subject>Discriminant Analysis</subject><subject>Discriminants</subject><subject>DNA</subject><subject>DNA fingerprints</subject><subject>Ecology</subject><subject>Essential amino acids</subject><subject>eukaryotes</subject><subject>Flowers & plants</subject><subject>food webs</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fungi</subject><subject>Fungi - chemistry</subject><subject>Fungi - classification</subject><subject>gas chromatography</subject><subject>gas chromatography-combustion-isotope ratio mass spectrometry (GC-C-IRMS)</subject><subject>General aspects</subject><subject>Isotopes</subject><subject>mass spectrometry</subject><subject>nutrients</subject><subject>Plants</subject><subject>Plants - chemistry</subject><subject>Plants - classification</subject><subject>prokaryotes</subject><subject>Species Specificity</subject><subject>Spectrometry, Mass, Electrospray Ionization - methods</subject><subject>stable isotopes</subject><subject>Taxa</subject><subject>trophic relationships</subject><subject>USA</subject><subject>δ13C</subject><issn>0012-9658</issn><issn>1939-9170</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kcuKFDEUhoMoTju68AHUIKgIU2PulcxuaMYLDLgYZ-GqSKeSNk1VpUyqlH57T1OtA4Jmk4T_O_-5IfSUknOqDXlHdEWVkef0HlpRw01laE3uoxUhlFVGSX2CHpWyI3Co0A_RCQOBaGpWaLyZ7KbzOJY0pdHjEIetz2OOwwSvC2zxkH74Dvd--pZaHFLGsfUghj3oeOzsMJ3hMA9b251hUDfWTT5H28EnbuNQcArY9nFI2LrYlsfoQbBd8U-O9ym6fX_1Zf2xuv784dP68rpyUilVmRCIcEIJzjQTtK4pc0YryZh0TrLQckNNWztrdCvaWnAlGIyCSE0oFcTwU_Rm8R1z-j77MjV9LM53ULBPc2lqLpiqqdZAvv4vySiTmisC4Mu_wF2a8wBdAKON0orUAL1dIJdTKdmHBobZ27xvKGkO22qIbg7baiiwz4-G86b37R_y93oAeHUEbHG2C9kOLpY7DqYhqDwYqYX7GTu__3fG5mr9lRFiDCTgkikIfLYE7sqU8p0x7ACoQzcvFj3Y1NhthuS3N1AeJ1RzUyvGfwF7BruY</recordid><startdate>200912</startdate><enddate>200912</enddate><creator>Larsen, Thomas</creator><creator>Taylor, D. Lee</creator><creator>Leigh, Mary Beth</creator><creator>O'Brien, Diane M</creator><general>Ecological Society of America</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7SN</scope><scope>7SS</scope><scope>7ST</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>SOI</scope><scope>7QL</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>200912</creationdate><title>Stable isotope fingerprinting: a novel method for identifying plant, fungal, or bacterial origins of amino acids</title><author>Larsen, Thomas ; Taylor, D. Lee ; Leigh, Mary Beth ; O'Brien, Diane M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5666-9ff04c4643282417712c9865225cc52fd3919d7ca98d4d7436428900580114093</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Alaska</topic><topic>Amino acids</topic><topic>Amino Acids - analysis</topic><topic>Amino Acids - chemistry</topic><topic>Animal and plant ecology</topic><topic>Animal, plant and microbial ecology</topic><topic>Bacteria</topic><topic>Bacteria - chemistry</topic><topic>Bacteria - classification</topic><topic>Biological and medical sciences</topic><topic>Biosynthesis</topic><topic>Boreal forests</topic><topic>carbon</topic><topic>Carbon Isotopes - analysis</topic><topic>Carbon Isotopes - chemistry</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>compound-specific stable isotope analysis</topic><topic>Discriminant Analysis</topic><topic>Discriminants</topic><topic>DNA</topic><topic>DNA fingerprints</topic><topic>Ecology</topic><topic>Essential amino acids</topic><topic>eukaryotes</topic><topic>Flowers & plants</topic><topic>food webs</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fungi</topic><topic>Fungi - chemistry</topic><topic>Fungi - classification</topic><topic>gas chromatography</topic><topic>gas chromatography-combustion-isotope ratio mass spectrometry (GC-C-IRMS)</topic><topic>General aspects</topic><topic>Isotopes</topic><topic>mass spectrometry</topic><topic>nutrients</topic><topic>Plants</topic><topic>Plants - chemistry</topic><topic>Plants - classification</topic><topic>prokaryotes</topic><topic>Species Specificity</topic><topic>Spectrometry, Mass, Electrospray Ionization - methods</topic><topic>stable isotopes</topic><topic>Taxa</topic><topic>trophic relationships</topic><topic>USA</topic><topic>δ13C</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Larsen, Thomas</creatorcontrib><creatorcontrib>Taylor, D. 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Lee</au><au>Leigh, Mary Beth</au><au>O'Brien, Diane M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Stable isotope fingerprinting: a novel method for identifying plant, fungal, or bacterial origins of amino acids</atitle><jtitle>Ecology (Durham)</jtitle><addtitle>Ecology</addtitle><date>2009-12</date><risdate>2009</risdate><volume>90</volume><issue>12</issue><spage>3526</spage><epage>3535</epage><pages>3526-3535</pages><issn>0012-9658</issn><eissn>1939-9170</eissn><coden>ECGYAQ</coden><abstract>Amino acids play an important role in ecology as essential nutrients for animals and as currencies in symbiotic associations. Here we present a new approach to tracing the origins of amino acids by identifying unique patterns of carbon isotope signatures generated by amino acid synthesis in plants, fungi, and bacteria ("¹³C fingerprints"). We measured amino acid δ¹³C from 10 C₃ plants, 13 fungi, and 10 bacteria collected and isolated from a boreal forest in interior Alaska, USA, using gas chromatography-combustion-isotope ratio mass spectrometry (GC-C-IRMS). Microorganisms were cultured under amino-acid-free conditions and identified based on DNA sequences. Bacteria, fungi, and plants generated consistent, unique ¹³C fingerprints based on the more complex amino acids (five or more biosynthetic steps) that are classified as essential for animals. Linear discriminant analysis classified all samples correctly with >99% certainty and correctly classified nearly all insect samples from a previous study by diet. Our results suggest that ¹³C fingerprints of amino acids could provide a powerful in situ assay of the biosynthetic sources of amino acids and a potential new tool for understanding nutritional linkages in food webs.</abstract><cop>Washington, DC</cop><pub>Ecological Society of America</pub><pmid>20120819</pmid><doi>10.1890/08-1695.1</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0012-9658 |
| ispartof | Ecology (Durham), 2009-12, Vol.90 (12), p.3526-3535 |
| issn | 0012-9658 1939-9170 |
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| source | MEDLINE; JSTOR Archive Collection A-Z Listing; Wiley Online Library All Journals |
| subjects | Alaska Amino acids Amino Acids - analysis Amino Acids - chemistry Animal and plant ecology Animal, plant and microbial ecology Bacteria Bacteria - chemistry Bacteria - classification Biological and medical sciences Biosynthesis Boreal forests carbon Carbon Isotopes - analysis Carbon Isotopes - chemistry Chromatography, High Pressure Liquid - methods compound-specific stable isotope analysis Discriminant Analysis Discriminants DNA DNA fingerprints Ecology Essential amino acids eukaryotes Flowers & plants food webs Fundamental and applied biological sciences. Psychology Fungi Fungi - chemistry Fungi - classification gas chromatography gas chromatography-combustion-isotope ratio mass spectrometry (GC-C-IRMS) General aspects Isotopes mass spectrometry nutrients Plants Plants - chemistry Plants - classification prokaryotes Species Specificity Spectrometry, Mass, Electrospray Ionization - methods stable isotopes Taxa trophic relationships USA δ13C |
| title | Stable isotope fingerprinting: a novel method for identifying plant, fungal, or bacterial origins of amino acids |
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