The mechanically enhanced phase separation of sprayed polyurethane scaffolds and their effect on the alignment of fibroblasts
Abstract This paper reports a method to fabricate anisotropic scaffolds of tunable porosity and mechanical properties. Scaffolds were fabricated using a computer controlled sprayed phase separation technique. Following fabrication, the sheets were elongated 0, 35 or 70% of their original length to i...
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| Veröffentlicht in: | Biomaterials 2010-02, Vol.31 (6), p.1126-1132 |
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| container_title | Biomaterials |
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| creator | Kennedy, James P McCandless, Sean P Lasher, Richard A Hitchcock, Robert W |
| description | Abstract This paper reports a method to fabricate anisotropic scaffolds of tunable porosity and mechanical properties. Scaffolds were fabricated using a computer controlled sprayed phase separation technique. Following fabrication, the sheets were elongated 0, 35 or 70% of their original length to induce varying degrees of scaffold alignment and anisotropy. The nonsolvent used in the phase separation was shown to affect porosity and the elastic modulus. Mouse embryo NIH-3T3 fibroblasts were cultured on the scaffolds to investigate cell response to the anisotropy of the scaffold. A 2D FFT method was used to quantify cellular alignment. Cells were shown to align themselves with the scaffold. This sheet-like scaffold material can be used in single plys or can be laminated to form porous 3D composite scaffolds. |
| doi_str_mv | 10.1016/j.biomaterials.2009.10.024 |
| format | Article |
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Scaffolds were fabricated using a computer controlled sprayed phase separation technique. Following fabrication, the sheets were elongated 0, 35 or 70% of their original length to induce varying degrees of scaffold alignment and anisotropy. The nonsolvent used in the phase separation was shown to affect porosity and the elastic modulus. Mouse embryo NIH-3T3 fibroblasts were cultured on the scaffolds to investigate cell response to the anisotropy of the scaffold. A 2D FFT method was used to quantify cellular alignment. Cells were shown to align themselves with the scaffold. This sheet-like scaffold material can be used in single plys or can be laminated to form porous 3D composite scaffolds.</description><identifier>ISSN: 0142-9612</identifier><identifier>EISSN: 1878-5905</identifier><identifier>DOI: 10.1016/j.biomaterials.2009.10.024</identifier><identifier>PMID: 19878993</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>3T3 Cells ; Absorption ; Advanced Basic Science ; Animals ; Anisotropy ; Biocompatible Materials - chemistry ; Biomimetic Materials - chemistry ; Cell alignment ; Cell Culture Techniques - methods ; Cell Polarity ; Crystallization - methods ; Dentistry ; Elastic Modulus ; Fibroblasts - cytology ; Fibroblasts - physiology ; Gases - chemistry ; Materials Testing ; Mechanical properties ; Mice ; Particle Size ; Phase Transition ; Polyurethane ; Polyurethanes - chemistry ; Porosity ; Scaffold ; Surface Properties ; Tensile Strength ; Tissue engineering ; Tissue Engineering - methods</subject><ispartof>Biomaterials, 2010-02, Vol.31 (6), p.1126-1132</ispartof><rights>2009</rights><rights>(c) 2009 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c434t-dcd8afcf5dc3d64dc13ad5139430db51117f83583b4d1b1a9e89bdd9a97c7d23</citedby><cites>FETCH-LOGICAL-c434t-dcd8afcf5dc3d64dc13ad5139430db51117f83583b4d1b1a9e89bdd9a97c7d23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.biomaterials.2009.10.024$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19878993$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kennedy, James P</creatorcontrib><creatorcontrib>McCandless, Sean P</creatorcontrib><creatorcontrib>Lasher, Richard A</creatorcontrib><creatorcontrib>Hitchcock, Robert W</creatorcontrib><title>The mechanically enhanced phase separation of sprayed polyurethane scaffolds and their effect on the alignment of fibroblasts</title><title>Biomaterials</title><addtitle>Biomaterials</addtitle><description>Abstract This paper reports a method to fabricate anisotropic scaffolds of tunable porosity and mechanical properties. Scaffolds were fabricated using a computer controlled sprayed phase separation technique. Following fabrication, the sheets were elongated 0, 35 or 70% of their original length to induce varying degrees of scaffold alignment and anisotropy. The nonsolvent used in the phase separation was shown to affect porosity and the elastic modulus. Mouse embryo NIH-3T3 fibroblasts were cultured on the scaffolds to investigate cell response to the anisotropy of the scaffold. A 2D FFT method was used to quantify cellular alignment. Cells were shown to align themselves with the scaffold. This sheet-like scaffold material can be used in single plys or can be laminated to form porous 3D composite scaffolds.</description><subject>3T3 Cells</subject><subject>Absorption</subject><subject>Advanced Basic Science</subject><subject>Animals</subject><subject>Anisotropy</subject><subject>Biocompatible Materials - chemistry</subject><subject>Biomimetic Materials - chemistry</subject><subject>Cell alignment</subject><subject>Cell Culture Techniques - methods</subject><subject>Cell Polarity</subject><subject>Crystallization - methods</subject><subject>Dentistry</subject><subject>Elastic Modulus</subject><subject>Fibroblasts - cytology</subject><subject>Fibroblasts - physiology</subject><subject>Gases - chemistry</subject><subject>Materials Testing</subject><subject>Mechanical properties</subject><subject>Mice</subject><subject>Particle Size</subject><subject>Phase Transition</subject><subject>Polyurethane</subject><subject>Polyurethanes - chemistry</subject><subject>Porosity</subject><subject>Scaffold</subject><subject>Surface Properties</subject><subject>Tensile Strength</subject><subject>Tissue engineering</subject><subject>Tissue Engineering - methods</subject><issn>0142-9612</issn><issn>1878-5905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkk2PFCEQhonRuLOrf8EQL556hKa_8GBiVldNNvHg3AkNhcNINy3Qm_TB_y44k2g8eYJKvW8V9VAIvaRkTwntXp_2o_WTTBCsdHFfE8JzYk_q5hHa0aEfqpaT9jHaEdrUFe9ofYWuYzyRHJOmfoquKM8qztkO_TwcAU-gjnK2Sjq3YZjzXYHGy1FGwBEWGWSyfsbe4LgEuZWcd9saIGVplihpjHc6YjlrnI5gAwZjQCWcXTnG0tlv8wRzKjWMHYMfnYwpPkNPTB4Bnl_OG3S4-3C4_VTdf_n4-fbdfaUa1qRKKz1Io0yrFdNdoxVlUreU8YYRPbaU0t4MrB3Y2Gg6Uslh4KPWXPJe9bpmN-jVuewS_I8VYhKTjQqcy6_3axQ9a-qO9G2XlW_OShV8jAGMWIKdZNgEJaLAFyfxN3xR4Jdchp_NLy5t1nEC_cd6oZ0F788CyLM-WAgiKgsFtg2ZltDe_l-ft_-UUc7-_r7vsEE8-TXMxUNFrAURX8salC0gnGRUlLNfbVq1PA</recordid><startdate>20100201</startdate><enddate>20100201</enddate><creator>Kennedy, James P</creator><creator>McCandless, Sean P</creator><creator>Lasher, Richard A</creator><creator>Hitchcock, Robert W</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20100201</creationdate><title>The mechanically enhanced phase separation of sprayed polyurethane scaffolds and their effect on the alignment of fibroblasts</title><author>Kennedy, James P ; 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| fulltext | fulltext |
| identifier | ISSN: 0142-9612 |
| ispartof | Biomaterials, 2010-02, Vol.31 (6), p.1126-1132 |
| issn | 0142-9612 1878-5905 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | 3T3 Cells Absorption Advanced Basic Science Animals Anisotropy Biocompatible Materials - chemistry Biomimetic Materials - chemistry Cell alignment Cell Culture Techniques - methods Cell Polarity Crystallization - methods Dentistry Elastic Modulus Fibroblasts - cytology Fibroblasts - physiology Gases - chemistry Materials Testing Mechanical properties Mice Particle Size Phase Transition Polyurethane Polyurethanes - chemistry Porosity Scaffold Surface Properties Tensile Strength Tissue engineering Tissue Engineering - methods |
| title | The mechanically enhanced phase separation of sprayed polyurethane scaffolds and their effect on the alignment of fibroblasts |
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