Comparative evaluation of the swelling and degrees of cross-linking in three organic gel packings for SEC through some geometric parameters
The size exclusion chromatographic (SEC) behavior of five solvent/polymer systems in three organic column packings based on polystyrene/divinylbenzene (PS/DVB) copolymer, TSK-Gel H HR, μ-styragel and TSK-Gel H XL, has been compared. All the packings offer similar characteristics (pore size, particle...
Gespeichert in:
| Veröffentlicht in: | Journal of biochemical and biophysical methods 2003-06, Vol.56 (1), p.53-67 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 67 |
|---|---|
| container_issue | 1 |
| container_start_page | 53 |
| container_title | Journal of biochemical and biophysical methods |
| container_volume | 56 |
| creator | Garcı́a, Rosa Gómez, Clara M Codoñer, Armando Abad, Concepción Campos, Agustı́n |
| description | The size exclusion chromatographic (SEC) behavior of five solvent/polymer systems in three organic column packings based on polystyrene/divinylbenzene (PS/DVB) copolymer, TSK-Gel H
HR, μ-styragel and TSK-Gel H
XL, has been compared. All the packings offer similar characteristics (pore size, particle size and efficiency) but some differences have been found when eluting the same systems. The different elution behavior observed in both polymeric gels has been analyzed in terms of their swelling and cross-linking degrees and of the fractal parameters. From the Universal Calibration plots, values of the chromatographic partition coefficient,
K
p, have been obtained and using some equations previously reported, values of the volume fraction of the network in the swollen state have been determined for the three sets of columns. Overall, for a given hydrodynamic volume and solvent-polymeric solute system the fraction of cross-linked polymer in the stationary phase was ordered according to: TSK-Gel H
XL>μ-styragel>TSK-Gel H
HR. This means an enhanced swelling degree for TSK-Gel H
HR. In general, fractal calculations support the thermodynamic predictions since both the fractal dimension and the pore size can be ordered as TSK-Gel H
HR>μ-styragel>TSK-Gel H
XL (in 10 of the 15 situations studied). The exceptions can be explained in terms of strong preferential solvation. |
| doi_str_mv | 10.1016/S0165-022X(03)00072-1 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_73425830</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0165022X03000721</els_id><sourcerecordid>73425830</sourcerecordid><originalsourceid>FETCH-LOGICAL-c363t-87d9b29836db4aacd3fde0a6d85eebf132a0f1ce857e3acf14ce3f00fcf279c83</originalsourceid><addsrcrecordid>eNqFkctOHDEQRa0oUZiQfEIir6KwaOJHPzwrhEaQREJiQSKxszx2uXHobg9296B8Q36a6pkRLNmUS7qnfFV1CfnM2SlnvP5-g6UqmBC335g8YYw1ouBvyIIrbFTV3L4li2fkiHzI-S9CUonyPTniQslyWasF-b-K_cYkM4YtUNiabsI2DjR6Ot4BzY_QdWFoqRkcddAmgDxrNsWcC1TuZzEMCKNEY2rNECxtoaMbY2cxUx8TvblYzUic2juaYw9IYB0TsrM7tpDyR_LOmy7Dp8N7TP5cXvxe_Syurn_8Wp1fFVbWcixU45ZrsVSyduvSGOukd8BM7VQFsPZcCsM8t4BHAGms56UF6Rnz1otmaZU8Jl_3_25SfJggj7oP2eKiZoA4Zd3IUlRKMgSrPbhbN4HXmxR6k_5pzvScgt6loOcTayb1LgXNce7LwWBa9-Bepg5nR-BsDwCuuQ2QdLYBBgsuJLCjdjG8YvEEYuubcQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>73425830</pqid></control><display><type>article</type><title>Comparative evaluation of the swelling and degrees of cross-linking in three organic gel packings for SEC through some geometric parameters</title><source>MEDLINE</source><source>Alma/SFX Local Collection</source><creator>Garcı́a, Rosa ; Gómez, Clara M ; Codoñer, Armando ; Abad, Concepción ; Campos, Agustı́n</creator><creatorcontrib>Garcı́a, Rosa ; Gómez, Clara M ; Codoñer, Armando ; Abad, Concepción ; Campos, Agustı́n</creatorcontrib><description>The size exclusion chromatographic (SEC) behavior of five solvent/polymer systems in three organic column packings based on polystyrene/divinylbenzene (PS/DVB) copolymer, TSK-Gel H
HR, μ-styragel and TSK-Gel H
XL, has been compared. All the packings offer similar characteristics (pore size, particle size and efficiency) but some differences have been found when eluting the same systems. The different elution behavior observed in both polymeric gels has been analyzed in terms of their swelling and cross-linking degrees and of the fractal parameters. From the Universal Calibration plots, values of the chromatographic partition coefficient,
K
p, have been obtained and using some equations previously reported, values of the volume fraction of the network in the swollen state have been determined for the three sets of columns. Overall, for a given hydrodynamic volume and solvent-polymeric solute system the fraction of cross-linked polymer in the stationary phase was ordered according to: TSK-Gel H
XL>μ-styragel>TSK-Gel H
HR. This means an enhanced swelling degree for TSK-Gel H
HR. In general, fractal calculations support the thermodynamic predictions since both the fractal dimension and the pore size can be ordered as TSK-Gel H
HR>μ-styragel>TSK-Gel H
XL (in 10 of the 15 situations studied). The exceptions can be explained in terms of strong preferential solvation.</description><identifier>ISSN: 0165-022X</identifier><identifier>EISSN: 1872-857X</identifier><identifier>DOI: 10.1016/S0165-022X(03)00072-1</identifier><identifier>PMID: 12834968</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Calibration ; Chromatography, Gel - instrumentation ; Chromatography, Gel - methods ; Cross-Linking Reagents - chemistry ; Equipment Failure Analysis ; Fractal behavior ; Gels - chemistry ; Macromolecular Substances ; Materials Testing - methods ; Molecular Conformation ; Organic Chemicals - chemistry ; Particle Size ; Porosity ; Size exclusion chromatography ; Swelling and cross-linking degree ; Viscosity</subject><ispartof>Journal of biochemical and biophysical methods, 2003-06, Vol.56 (1), p.53-67</ispartof><rights>2003 Elsevier Science B.V.</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c363t-87d9b29836db4aacd3fde0a6d85eebf132a0f1ce857e3acf14ce3f00fcf279c83</citedby><cites>FETCH-LOGICAL-c363t-87d9b29836db4aacd3fde0a6d85eebf132a0f1ce857e3acf14ce3f00fcf279c83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12834968$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Garcı́a, Rosa</creatorcontrib><creatorcontrib>Gómez, Clara M</creatorcontrib><creatorcontrib>Codoñer, Armando</creatorcontrib><creatorcontrib>Abad, Concepción</creatorcontrib><creatorcontrib>Campos, Agustı́n</creatorcontrib><title>Comparative evaluation of the swelling and degrees of cross-linking in three organic gel packings for SEC through some geometric parameters</title><title>Journal of biochemical and biophysical methods</title><addtitle>J Biochem Biophys Methods</addtitle><description>The size exclusion chromatographic (SEC) behavior of five solvent/polymer systems in three organic column packings based on polystyrene/divinylbenzene (PS/DVB) copolymer, TSK-Gel H
HR, μ-styragel and TSK-Gel H
XL, has been compared. All the packings offer similar characteristics (pore size, particle size and efficiency) but some differences have been found when eluting the same systems. The different elution behavior observed in both polymeric gels has been analyzed in terms of their swelling and cross-linking degrees and of the fractal parameters. From the Universal Calibration plots, values of the chromatographic partition coefficient,
K
p, have been obtained and using some equations previously reported, values of the volume fraction of the network in the swollen state have been determined for the three sets of columns. Overall, for a given hydrodynamic volume and solvent-polymeric solute system the fraction of cross-linked polymer in the stationary phase was ordered according to: TSK-Gel H
XL>μ-styragel>TSK-Gel H
HR. This means an enhanced swelling degree for TSK-Gel H
HR. In general, fractal calculations support the thermodynamic predictions since both the fractal dimension and the pore size can be ordered as TSK-Gel H
HR>μ-styragel>TSK-Gel H
XL (in 10 of the 15 situations studied). The exceptions can be explained in terms of strong preferential solvation.</description><subject>Calibration</subject><subject>Chromatography, Gel - instrumentation</subject><subject>Chromatography, Gel - methods</subject><subject>Cross-Linking Reagents - chemistry</subject><subject>Equipment Failure Analysis</subject><subject>Fractal behavior</subject><subject>Gels - chemistry</subject><subject>Macromolecular Substances</subject><subject>Materials Testing - methods</subject><subject>Molecular Conformation</subject><subject>Organic Chemicals - chemistry</subject><subject>Particle Size</subject><subject>Porosity</subject><subject>Size exclusion chromatography</subject><subject>Swelling and cross-linking degree</subject><subject>Viscosity</subject><issn>0165-022X</issn><issn>1872-857X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkctOHDEQRa0oUZiQfEIir6KwaOJHPzwrhEaQREJiQSKxszx2uXHobg9296B8Q36a6pkRLNmUS7qnfFV1CfnM2SlnvP5-g6UqmBC335g8YYw1ouBvyIIrbFTV3L4li2fkiHzI-S9CUonyPTniQslyWasF-b-K_cYkM4YtUNiabsI2DjR6Ot4BzY_QdWFoqRkcddAmgDxrNsWcC1TuZzEMCKNEY2rNECxtoaMbY2cxUx8TvblYzUic2juaYw9IYB0TsrM7tpDyR_LOmy7Dp8N7TP5cXvxe_Syurn_8Wp1fFVbWcixU45ZrsVSyduvSGOukd8BM7VQFsPZcCsM8t4BHAGms56UF6Rnz1otmaZU8Jl_3_25SfJggj7oP2eKiZoA4Zd3IUlRKMgSrPbhbN4HXmxR6k_5pzvScgt6loOcTayb1LgXNce7LwWBa9-Bepg5nR-BsDwCuuQ2QdLYBBgsuJLCjdjG8YvEEYuubcQ</recordid><startdate>20030630</startdate><enddate>20030630</enddate><creator>Garcı́a, Rosa</creator><creator>Gómez, Clara M</creator><creator>Codoñer, Armando</creator><creator>Abad, Concepción</creator><creator>Campos, Agustı́n</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20030630</creationdate><title>Comparative evaluation of the swelling and degrees of cross-linking in three organic gel packings for SEC through some geometric parameters</title><author>Garcı́a, Rosa ; Gómez, Clara M ; Codoñer, Armando ; Abad, Concepción ; Campos, Agustı́n</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c363t-87d9b29836db4aacd3fde0a6d85eebf132a0f1ce857e3acf14ce3f00fcf279c83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Calibration</topic><topic>Chromatography, Gel - instrumentation</topic><topic>Chromatography, Gel - methods</topic><topic>Cross-Linking Reagents - chemistry</topic><topic>Equipment Failure Analysis</topic><topic>Fractal behavior</topic><topic>Gels - chemistry</topic><topic>Macromolecular Substances</topic><topic>Materials Testing - methods</topic><topic>Molecular Conformation</topic><topic>Organic Chemicals - chemistry</topic><topic>Particle Size</topic><topic>Porosity</topic><topic>Size exclusion chromatography</topic><topic>Swelling and cross-linking degree</topic><topic>Viscosity</topic><toplevel>online_resources</toplevel><creatorcontrib>Garcı́a, Rosa</creatorcontrib><creatorcontrib>Gómez, Clara M</creatorcontrib><creatorcontrib>Codoñer, Armando</creatorcontrib><creatorcontrib>Abad, Concepción</creatorcontrib><creatorcontrib>Campos, Agustı́n</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of biochemical and biophysical methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Garcı́a, Rosa</au><au>Gómez, Clara M</au><au>Codoñer, Armando</au><au>Abad, Concepción</au><au>Campos, Agustı́n</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparative evaluation of the swelling and degrees of cross-linking in three organic gel packings for SEC through some geometric parameters</atitle><jtitle>Journal of biochemical and biophysical methods</jtitle><addtitle>J Biochem Biophys Methods</addtitle><date>2003-06-30</date><risdate>2003</risdate><volume>56</volume><issue>1</issue><spage>53</spage><epage>67</epage><pages>53-67</pages><issn>0165-022X</issn><eissn>1872-857X</eissn><abstract>The size exclusion chromatographic (SEC) behavior of five solvent/polymer systems in three organic column packings based on polystyrene/divinylbenzene (PS/DVB) copolymer, TSK-Gel H
HR, μ-styragel and TSK-Gel H
XL, has been compared. All the packings offer similar characteristics (pore size, particle size and efficiency) but some differences have been found when eluting the same systems. The different elution behavior observed in both polymeric gels has been analyzed in terms of their swelling and cross-linking degrees and of the fractal parameters. From the Universal Calibration plots, values of the chromatographic partition coefficient,
K
p, have been obtained and using some equations previously reported, values of the volume fraction of the network in the swollen state have been determined for the three sets of columns. Overall, for a given hydrodynamic volume and solvent-polymeric solute system the fraction of cross-linked polymer in the stationary phase was ordered according to: TSK-Gel H
XL>μ-styragel>TSK-Gel H
HR. This means an enhanced swelling degree for TSK-Gel H
HR. In general, fractal calculations support the thermodynamic predictions since both the fractal dimension and the pore size can be ordered as TSK-Gel H
HR>μ-styragel>TSK-Gel H
XL (in 10 of the 15 situations studied). The exceptions can be explained in terms of strong preferential solvation.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>12834968</pmid><doi>10.1016/S0165-022X(03)00072-1</doi><tpages>15</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0165-022X |
| ispartof | Journal of biochemical and biophysical methods, 2003-06, Vol.56 (1), p.53-67 |
| issn | 0165-022X 1872-857X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_73425830 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Calibration Chromatography, Gel - instrumentation Chromatography, Gel - methods Cross-Linking Reagents - chemistry Equipment Failure Analysis Fractal behavior Gels - chemistry Macromolecular Substances Materials Testing - methods Molecular Conformation Organic Chemicals - chemistry Particle Size Porosity Size exclusion chromatography Swelling and cross-linking degree Viscosity |
| title | Comparative evaluation of the swelling and degrees of cross-linking in three organic gel packings for SEC through some geometric parameters |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-13T15%3A20%3A24IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Comparative%20evaluation%20of%20the%20swelling%20and%20degrees%20of%20cross-linking%20in%20three%20organic%20gel%20packings%20for%20SEC%20through%20some%20geometric%20parameters&rft.jtitle=Journal%20of%20biochemical%20and%20biophysical%20methods&rft.au=Garc%C4%B1%CC%81a,%20Rosa&rft.date=2003-06-30&rft.volume=56&rft.issue=1&rft.spage=53&rft.epage=67&rft.pages=53-67&rft.issn=0165-022X&rft.eissn=1872-857X&rft_id=info:doi/10.1016/S0165-022X(03)00072-1&rft_dat=%3Cproquest_cross%3E73425830%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=73425830&rft_id=info:pmid/12834968&rft_els_id=S0165022X03000721&rfr_iscdi=true |