Micropropagation of Thapsia garganica: a medicinal plant
The micropropagation of T. garganica, a medicinally important plant, was investigated as an option for conservation purposes as wild populations are becoming sparse. A Murashige and Skoog agar medium supplemented with 0.5 mg l(-1) alpha-naphthaleneacetic acid and 1.5 mg l(-1) benzyl-6-adenine signif...
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| Veröffentlicht in: | Plant cell reports 2003-06, Vol.21 (10), p.967-973 |
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| creator | MAKUNGA, N. P JÄGER, A. K VAN STADEN, J |
| description | The micropropagation of T. garganica, a medicinally important plant, was investigated as an option for conservation purposes as wild populations are becoming sparse. A Murashige and Skoog agar medium supplemented with 0.5 mg l(-1) alpha-naphthaleneacetic acid and 1.5 mg l(-1) benzyl-6-adenine significantly improved the production of multiple shoots directly from petiole and leaflet explants compared to other plant growth regulator (PGR) combinations. Medium free of PGRs promoted rooting at a low incidence. Acclimatisation was low--52% plantlet survival--as micropropagated plantlets were highly susceptible to fungal rot once removed from culture. Application of antifungal agents to in vitro-derived plantlets as pre- and post-acclimatisation treatments during transplanting significantly reduced ex vitro mortality. |
| doi_str_mv | 10.1007/s00299-003-0623-8 |
| format | Article |
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| ispartof | Plant cell reports, 2003-06, Vol.21 (10), p.967-973 |
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| source | MEDLINE; SpringerNature Journals |
| subjects | Acclimatization Antifungal agents Apiaceae - growth & development Apiaceae - microbiology Biological and medical sciences Culture Techniques Fundamental and applied biological sciences. Psychology Growth regulators Medicinal plants Plant growth Plant Roots - growth & development Plant Shoots - growth & development Plants, Medicinal - growth & development Regeneration Reproduction Thapsia garganica |
| title | Micropropagation of Thapsia garganica: a medicinal plant |
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