Effect of decellular treatment on the framework of extracellular matrix in bovine jugular vein conduit
To investigate the effect of decellular treatment on the framework constituents of extracellular matrix and tissue stability in bovine jugular vein conduit (BJVC), and to provide an evidence for tissue engineering of vascular prosthesis. Bovine jugular veins were obtained fresh from a local slaughte...
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| Veröffentlicht in: | Zhong nan da xue xue bao. Journal of Central South University. Yi xue ban 2007-10, Vol.32 (5), p.819-823 |
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| container_title | Zhong nan da xue xue bao. Journal of Central South University. Yi xue ban |
| container_volume | 32 |
| creator | Lu, Wei-dong Wu, Zhong-shi Hu, Tie-hui Zhang, Ming Hu, Ye-rong Jiang, Zhi-bin Li, Wei Liu, Zhe-liang Hu, Jian-guo |
| description | To investigate the effect of decellular treatment on the framework constituents of extracellular matrix and tissue stability in bovine jugular vein conduit (BJVC), and to provide an evidence for tissue engineering of vascular prosthesis.
Bovine jugular veins were obtained fresh from a local slaughterhouse and were stored in chilled PBS. In the laboratory, any fat and loose connective tissue on the outer surface of the vessel was trimmed. BJVCs were decellularized by a 3-step extraction method as detergent Triton X-100 (0.5%), Trypsin (0.025%) EDTA (0.02%), and DNase I(30kU/L) RNaseA(0.3g/L). Histological and transmission electron microscopy (TEM) techniques were used to study the framework constituents of extracellular matrix of treated the examples, and fresh tissues were used as controls. Tissue contents of hydroxyproline(alkaline hydrolysis method) and elastin (Fastin Elastin Assay) were assayed respectively in the fresh and decellularized groups (n=10). The vascular wall heat shrinking temperature and mec |
| format | Article |
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Bovine jugular veins were obtained fresh from a local slaughterhouse and were stored in chilled PBS. In the laboratory, any fat and loose connective tissue on the outer surface of the vessel was trimmed. BJVCs were decellularized by a 3-step extraction method as detergent Triton X-100 (0.5%), Trypsin (0.025%) EDTA (0.02%), and DNase I(30kU/L) RNaseA(0.3g/L). Histological and transmission electron microscopy (TEM) techniques were used to study the framework constituents of extracellular matrix of treated the examples, and fresh tissues were used as controls. Tissue contents of hydroxyproline(alkaline hydrolysis method) and elastin (Fastin Elastin Assay) were assayed respectively in the fresh and decellularized groups (n=10). The vascular wall heat shrinking temperature and mec</description><subject>Animals</subject><subject>Blood Vessel Prosthesis</subject><subject>Cattle</subject><subject>Extracellular Matrix</subject><subject>Jugular Veins</subject><subject>Tissue Engineering - methods</subject><subject>Tissue Scaffolds</subject><issn>1672-7347</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kMtOwzAURL0A0ar0F5B3rCL5FTteoqo8pEpsYB058TUY4jg4Til_TwqF2Vxp7tFoNGdoSaViheJCLdB6HH1DiNaay0pdoAWtCFFEqSVyW-egzTg6bKGFrps6k3BOYHKAfvZ7nF8Bu2QCfMb0fgThkJP5Z4PJyR-w73ET974H_Da9_Dz2MHtt7O3k8yU6d6YbYX26K_R8u33a3Be7x7uHzc2uGCgrc-FK0UgmtTLaajarIlpwW8m5ozVcOllZSWWjaClbpXgphAZJBCVguWwrvkLXv7lDih8TjLkOfjxWNT3EaaznPRgTlPKZvDqRUxPA1kPywaSv-m8a_g2KjWBH</recordid><startdate>200710</startdate><enddate>200710</enddate><creator>Lu, Wei-dong</creator><creator>Wu, Zhong-shi</creator><creator>Hu, Tie-hui</creator><creator>Zhang, Ming</creator><creator>Hu, Ye-rong</creator><creator>Jiang, Zhi-bin</creator><creator>Li, Wei</creator><creator>Liu, Zhe-liang</creator><creator>Hu, Jian-guo</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>200710</creationdate><title>Effect of decellular treatment on the framework of extracellular matrix in bovine jugular vein conduit</title><author>Lu, Wei-dong ; Wu, Zhong-shi ; Hu, Tie-hui ; Zhang, Ming ; Hu, Ye-rong ; Jiang, Zhi-bin ; Li, Wei ; Liu, Zhe-liang ; Hu, Jian-guo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p125t-f54b62697a9d9222280943d86fecda36f68d616b7156c7735449e60410ed36c83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>chi</language><creationdate>2007</creationdate><topic>Animals</topic><topic>Blood Vessel Prosthesis</topic><topic>Cattle</topic><topic>Extracellular Matrix</topic><topic>Jugular Veins</topic><topic>Tissue Engineering - methods</topic><topic>Tissue Scaffolds</topic><toplevel>online_resources</toplevel><creatorcontrib>Lu, Wei-dong</creatorcontrib><creatorcontrib>Wu, Zhong-shi</creatorcontrib><creatorcontrib>Hu, Tie-hui</creatorcontrib><creatorcontrib>Zhang, Ming</creatorcontrib><creatorcontrib>Hu, Ye-rong</creatorcontrib><creatorcontrib>Jiang, Zhi-bin</creatorcontrib><creatorcontrib>Li, Wei</creatorcontrib><creatorcontrib>Liu, Zhe-liang</creatorcontrib><creatorcontrib>Hu, Jian-guo</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Zhong nan da xue xue bao. Journal of Central South University. Yi xue ban</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lu, Wei-dong</au><au>Wu, Zhong-shi</au><au>Hu, Tie-hui</au><au>Zhang, Ming</au><au>Hu, Ye-rong</au><au>Jiang, Zhi-bin</au><au>Li, Wei</au><au>Liu, Zhe-liang</au><au>Hu, Jian-guo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of decellular treatment on the framework of extracellular matrix in bovine jugular vein conduit</atitle><jtitle>Zhong nan da xue xue bao. Journal of Central South University. Yi xue ban</jtitle><addtitle>Zhong Nan Da Xue Xue Bao Yi Xue Ban</addtitle><date>2007-10</date><risdate>2007</risdate><volume>32</volume><issue>5</issue><spage>819</spage><epage>823</epage><pages>819-823</pages><issn>1672-7347</issn><abstract>To investigate the effect of decellular treatment on the framework constituents of extracellular matrix and tissue stability in bovine jugular vein conduit (BJVC), and to provide an evidence for tissue engineering of vascular prosthesis.
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| ispartof | Zhong nan da xue xue bao. Journal of Central South University. Yi xue ban, 2007-10, Vol.32 (5), p.819-823 |
| issn | 1672-7347 |
| language | chi |
| recordid | cdi_proquest_miscellaneous_734224113 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Blood Vessel Prosthesis Cattle Extracellular Matrix Jugular Veins Tissue Engineering - methods Tissue Scaffolds |
| title | Effect of decellular treatment on the framework of extracellular matrix in bovine jugular vein conduit |
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