β-Glucosidase coating on polymer nanofibers for improved cellulosic ethanol production
β-Glucosidase (βG) can relieve the product inhibition of cellobiose in the cellulosic ethanol production by converting cellobiose into glucose. For the potential recycled uses, βG was immobilized and stabilized in the form of enzyme coating on polymer nanofibers. The βG coating (EC-βG) was fabricate...
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| Veröffentlicht in: | Bioprocess and biosystems engineering 2010, Vol.33 (1), p.141-147 |
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| container_title | Bioprocess and biosystems engineering |
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| creator | Lee, Sang-Mok Jin, Li Hua Kim, Jae Hyun Han, Sung Ok Na, Hyon Bin Hyeon, Taeghwan Koo, Yoon-Mo Kim, Jungbae Lee, Jung-Heon |
| description | β-Glucosidase (βG) can relieve the product inhibition of cellobiose in the cellulosic ethanol production by converting cellobiose into glucose. For the potential recycled uses, βG was immobilized and stabilized in the form of enzyme coating on polymer nanofibers. The βG coating (EC-βG) was fabricated by crosslinking additional βG molecules onto covalently attached βG molecules (CA-βG) via glutaraldehyde treatment. The initial activity of EC-βG was 36 times higher than that of CA-βG. After 20 days of incubation under shaking, CA-βG and EC-βG retained 33 and 91% of each initial activity, respectively. Magnetic nanofibers were also used for easy recovery and recycled uses of βG coating. It is anticipated that the recycled uses of highly active and stable βG coating can improve the economics of cellulosic ethanol production so long as economical materials are employed as a host of enzyme immobilization. |
| doi_str_mv | 10.1007/s00449-009-0386-x |
| format | Article |
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For the potential recycled uses, βG was immobilized and stabilized in the form of enzyme coating on polymer nanofibers. The βG coating (EC-βG) was fabricated by crosslinking additional βG molecules onto covalently attached βG molecules (CA-βG) via glutaraldehyde treatment. The initial activity of EC-βG was 36 times higher than that of CA-βG. After 20 days of incubation under shaking, CA-βG and EC-βG retained 33 and 91% of each initial activity, respectively. Magnetic nanofibers were also used for easy recovery and recycled uses of βG coating. 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For the potential recycled uses, βG was immobilized and stabilized in the form of enzyme coating on polymer nanofibers. The βG coating (EC-βG) was fabricated by crosslinking additional βG molecules onto covalently attached βG molecules (CA-βG) via glutaraldehyde treatment. The initial activity of EC-βG was 36 times higher than that of CA-βG. After 20 days of incubation under shaking, CA-βG and EC-βG retained 33 and 91% of each initial activity, respectively. Magnetic nanofibers were also used for easy recovery and recycled uses of βG coating. It is anticipated that the recycled uses of highly active and stable βG coating can improve the economics of cellulosic ethanol production so long as economical materials are employed as a host of enzyme immobilization.</description><subject>beta-Glucosidase - chemistry</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Cellobiose - chemistry</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Environmental Engineering/Biotechnology</subject><subject>Enzymes, Immobilized - chemistry</subject><subject>Ethanol</subject><subject>Food Science</subject><subject>Fundamental and applied biological sciences. 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| subjects | beta-Glucosidase - chemistry Biological and medical sciences Biotechnology Cellobiose - chemistry Chemistry Chemistry and Materials Science Environmental Engineering/Biotechnology Enzymes, Immobilized - chemistry Ethanol Food Science Fundamental and applied biological sciences. Psychology Glucose - chemistry Industrial and Production Engineering Industrial Chemistry/Chemical Engineering Nanofibers - chemistry Original Paper |
| title | β-Glucosidase coating on polymer nanofibers for improved cellulosic ethanol production |
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