Synthetic translational regulation by an L7Ae–kink-turn RNP switch

The ribosomal protein L7Ae and its RNA partner were used to construct a synthetic translational regulator that can be used to activate or repress protein expression, even performing both functions at once. Repression in human cells is highly effective, rivaling the potency of RNAi. The regulation of...

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Veröffentlicht in:	Nature chemical biology 2010-01, Vol.6 (1), p.71-78
Hauptverfasser:	Saito, Hirohide, Inoue, Tan, Kobayashi, Tetsuhiro, Hara, Tomoaki, Fujita, Yoshihiko, Hayashi, Karin, Furushima, Rie
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacillus subtilis - metabolism Biochemical Engineering Biochemistry Bioengineering - methods Bioorganic Chemistry Cell Biology Cell Line Cell-Free System Cellular biology Chemistry Chemistry and Materials Science Chemistry/Food Science Genetics Green Fluorescent Proteins - metabolism HeLa Cells Humans Kinetics Models, Biological Models, Genetic Protein Biosynthesis Protein Engineering - methods Ribosomal Proteins - chemistry Ribosomal Proteins - metabolism RNA - chemistry RNA, Messenger - metabolism Signal transduction Tissue engineering
Online-Zugang:	Volltext
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