Improvement of the Survival of Human Autologous Fat Transplantation by Using VEGF-Transfected Adipose-Derived Stem Cells
The efficacy of autologous fat transplantation is reduced by fat absorption and fibrosis due to fat necrosis. Enhanced transplant neovascularization early after transplantation may reduce these outcomes. The authors asked whether cell and concomitant gene therapy using adipose-derived stem cells tra...
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| Veröffentlicht in: | Plastic and reconstructive surgery (1963) 2009-11, Vol.124 (5), p.1437-1446 |
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| container_title | Plastic and reconstructive surgery (1963) |
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| creator | Lu, Feng Li, Jie Gao, JianHua Ogawa, Rei Ou, Chunquan Yang, Bo Fu, Bingchuan |
| description | The efficacy of autologous fat transplantation is reduced by fat absorption and fibrosis due to fat necrosis. Enhanced transplant neovascularization early after transplantation may reduce these outcomes. The authors asked whether cell and concomitant gene therapy using adipose-derived stem cells transduced with vascular endothelial growth factor (VEGF) improves fat transplant neovascularization and survival.
Human adipose-derived stem cells were expanded ex vivo for three passages, labeled with 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine (DiI), and transduced with VEGF or left untransduced. Human fat tissues were then mixed with the DiI-labeled VEGF-transduced adipose-derived stem cells, the DiI-labeled adipose-derived stem cells, the known vascularization-promoting agent insulin, or medium alone, and 18 nude mice were injected subcutaneously with all four preparations, with each of the four designated spots receiving one of these four mixtures in a random fashion. Six months later, transplanted tissue volume and histology were evaluated and neovascularization was quantified by counting the capillaries.
Control transplant survival was 27.1 +/- 8.2 percent, but mixture with the VEGF-transduced and VEGF-untransduced stem cells significantly increased transplant survival (74.1 +/- 12.6 percent and 60.1 +/- 17.6 percent, respectively). Insulin was less effective (37.7 +/- 6.9 percent). Histological analysis revealed both types of transplants consisted predominantly of adipose tissue, unlike the control transplants, and had significantly less fat necrosis and fibrosis. The VEGF-transduced, adipose-derived stem cell-treated transplants had significantly higher capillary density than the other transplants and bore DiI-double-positive and CD31-double-positive cells (i.e., adipose-derived stem cell-derived endothelial cells).
Adipose-derived stem cells together with VEGF transduction can enhance the survival and quality of transplanted fat tissues. |
| doi_str_mv | 10.1097/PRS.0b013e3181babbb6 |
| format | Article |
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Human adipose-derived stem cells were expanded ex vivo for three passages, labeled with 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine (DiI), and transduced with VEGF or left untransduced. Human fat tissues were then mixed with the DiI-labeled VEGF-transduced adipose-derived stem cells, the DiI-labeled adipose-derived stem cells, the known vascularization-promoting agent insulin, or medium alone, and 18 nude mice were injected subcutaneously with all four preparations, with each of the four designated spots receiving one of these four mixtures in a random fashion. Six months later, transplanted tissue volume and histology were evaluated and neovascularization was quantified by counting the capillaries.
Control transplant survival was 27.1 +/- 8.2 percent, but mixture with the VEGF-transduced and VEGF-untransduced stem cells significantly increased transplant survival (74.1 +/- 12.6 percent and 60.1 +/- 17.6 percent, respectively). Insulin was less effective (37.7 +/- 6.9 percent). Histological analysis revealed both types of transplants consisted predominantly of adipose tissue, unlike the control transplants, and had significantly less fat necrosis and fibrosis. The VEGF-transduced, adipose-derived stem cell-treated transplants had significantly higher capillary density than the other transplants and bore DiI-double-positive and CD31-double-positive cells (i.e., adipose-derived stem cell-derived endothelial cells).
Adipose-derived stem cells together with VEGF transduction can enhance the survival and quality of transplanted fat tissues.</description><identifier>ISSN: 0032-1052</identifier><identifier>EISSN: 1529-4242</identifier><identifier>DOI: 10.1097/PRS.0b013e3181babbb6</identifier><identifier>PMID: 20009828</identifier><language>eng</language><publisher>Hagerstown, MD: American Society of Plastic Surgeons</publisher><subject>Adenoviridae ; Adipocytes - transplantation ; Adipose Tissue - cytology ; Adipose Tissue - transplantation ; Animals ; Biological and medical sciences ; Gene Expression Regulation, Viral ; Graft Survival ; Humans ; Medical sciences ; Mice ; Mice, Nude ; Models, Animal ; Neovascularization, Physiologic - genetics ; Stem Cell Transplantation - methods ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Transduction, Genetic ; Transfection ; Transgenes ; Transplantation, Autologous ; Vascular Endothelial Growth Factor A - genetics</subject><ispartof>Plastic and reconstructive surgery (1963), 2009-11, Vol.124 (5), p.1437-1446</ispartof><rights>American Society of Plastic Surgeons</rights><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4479-343a6f1548ac2d817b672a08cf3c59250cf6c97ed5d85ccde726f703983cfc2e3</citedby><cites>FETCH-LOGICAL-c4479-343a6f1548ac2d817b672a08cf3c59250cf6c97ed5d85ccde726f703983cfc2e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22125134$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20009828$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lu, Feng</creatorcontrib><creatorcontrib>Li, Jie</creatorcontrib><creatorcontrib>Gao, JianHua</creatorcontrib><creatorcontrib>Ogawa, Rei</creatorcontrib><creatorcontrib>Ou, Chunquan</creatorcontrib><creatorcontrib>Yang, Bo</creatorcontrib><creatorcontrib>Fu, Bingchuan</creatorcontrib><title>Improvement of the Survival of Human Autologous Fat Transplantation by Using VEGF-Transfected Adipose-Derived Stem Cells</title><title>Plastic and reconstructive surgery (1963)</title><addtitle>Plast Reconstr Surg</addtitle><description>The efficacy of autologous fat transplantation is reduced by fat absorption and fibrosis due to fat necrosis. Enhanced transplant neovascularization early after transplantation may reduce these outcomes. The authors asked whether cell and concomitant gene therapy using adipose-derived stem cells transduced with vascular endothelial growth factor (VEGF) improves fat transplant neovascularization and survival.
Human adipose-derived stem cells were expanded ex vivo for three passages, labeled with 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine (DiI), and transduced with VEGF or left untransduced. Human fat tissues were then mixed with the DiI-labeled VEGF-transduced adipose-derived stem cells, the DiI-labeled adipose-derived stem cells, the known vascularization-promoting agent insulin, or medium alone, and 18 nude mice were injected subcutaneously with all four preparations, with each of the four designated spots receiving one of these four mixtures in a random fashion. Six months later, transplanted tissue volume and histology were evaluated and neovascularization was quantified by counting the capillaries.
Control transplant survival was 27.1 +/- 8.2 percent, but mixture with the VEGF-transduced and VEGF-untransduced stem cells significantly increased transplant survival (74.1 +/- 12.6 percent and 60.1 +/- 17.6 percent, respectively). Insulin was less effective (37.7 +/- 6.9 percent). Histological analysis revealed both types of transplants consisted predominantly of adipose tissue, unlike the control transplants, and had significantly less fat necrosis and fibrosis. The VEGF-transduced, adipose-derived stem cell-treated transplants had significantly higher capillary density than the other transplants and bore DiI-double-positive and CD31-double-positive cells (i.e., adipose-derived stem cell-derived endothelial cells).
Adipose-derived stem cells together with VEGF transduction can enhance the survival and quality of transplanted fat tissues.</description><subject>Adenoviridae</subject><subject>Adipocytes - transplantation</subject><subject>Adipose Tissue - cytology</subject><subject>Adipose Tissue - transplantation</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Gene Expression Regulation, Viral</subject><subject>Graft Survival</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Nude</subject><subject>Models, Animal</subject><subject>Neovascularization, Physiologic - genetics</subject><subject>Stem Cell Transplantation - methods</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</subject><subject>Transduction, Genetic</subject><subject>Transfection</subject><subject>Transgenes</subject><subject>Transplantation, Autologous</subject><subject>Vascular Endothelial Growth Factor A - genetics</subject><issn>0032-1052</issn><issn>1529-4242</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkF9v0zAUxS0EYmXwDRDyC-Ipw3_iOH6syrpNmgSiG6-W41yvAScuttOxb4_LypB4so79u-f6HITeUnJGiZIfv3zdnJGOUA6ctrQzXdc1z9CCCqaqmtXsOVoQwllFiWAn6FVK3wmhkjfiJTphhBDVsnaBfl2Nuxj2MMKUcXA4bwFv5rgf9sYf9OU8mgkv5xx8uAtzwmuT8U00U9p5M2WThzDh7gHfpmG6w9_OL9bVn1cHNkOPl_2wCwmqTxCHfdGbDCNegffpNXrhjE_w5nieotv1-c3qsrr-fHG1Wl5Xtq6lqnjNTeOoqFtjWd9S2TWSGdJax61QTBDrGqsk9KJvhbU9SNY4SbhquXWWAT9FHx59S8yfM6SsxyHZ8gMzQcmjJa-pkoI2hawfSRtDShGc3sVhNPFBU6IPletSuf6_8jL27rhg7kbon4b-dlyA90fAJGu8K_XYIf3jGGWClpxP---DzxDTDz_fQ9RbMD5vdXEjjeB1VXwVpUVVhyvFfwN73Jwb</recordid><startdate>20091101</startdate><enddate>20091101</enddate><creator>Lu, Feng</creator><creator>Li, Jie</creator><creator>Gao, JianHua</creator><creator>Ogawa, Rei</creator><creator>Ou, Chunquan</creator><creator>Yang, Bo</creator><creator>Fu, Bingchuan</creator><general>American Society of Plastic Surgeons</general><general>Lippincott Williams & Wilkins</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20091101</creationdate><title>Improvement of the Survival of Human Autologous Fat Transplantation by Using VEGF-Transfected Adipose-Derived Stem Cells</title><author>Lu, Feng ; Li, Jie ; Gao, JianHua ; Ogawa, Rei ; Ou, Chunquan ; Yang, Bo ; Fu, Bingchuan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4479-343a6f1548ac2d817b672a08cf3c59250cf6c97ed5d85ccde726f703983cfc2e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Adenoviridae</topic><topic>Adipocytes - transplantation</topic><topic>Adipose Tissue - cytology</topic><topic>Adipose Tissue - transplantation</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Gene Expression Regulation, Viral</topic><topic>Graft Survival</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Nude</topic><topic>Models, Animal</topic><topic>Neovascularization, Physiologic - genetics</topic><topic>Stem Cell Transplantation - methods</topic><topic>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</topic><topic>Transduction, Genetic</topic><topic>Transfection</topic><topic>Transgenes</topic><topic>Transplantation, Autologous</topic><topic>Vascular Endothelial Growth Factor A - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lu, Feng</creatorcontrib><creatorcontrib>Li, Jie</creatorcontrib><creatorcontrib>Gao, JianHua</creatorcontrib><creatorcontrib>Ogawa, Rei</creatorcontrib><creatorcontrib>Ou, Chunquan</creatorcontrib><creatorcontrib>Yang, Bo</creatorcontrib><creatorcontrib>Fu, Bingchuan</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Plastic and reconstructive surgery (1963)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lu, Feng</au><au>Li, Jie</au><au>Gao, JianHua</au><au>Ogawa, Rei</au><au>Ou, Chunquan</au><au>Yang, Bo</au><au>Fu, Bingchuan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Improvement of the Survival of Human Autologous Fat Transplantation by Using VEGF-Transfected Adipose-Derived Stem Cells</atitle><jtitle>Plastic and reconstructive surgery (1963)</jtitle><addtitle>Plast Reconstr Surg</addtitle><date>2009-11-01</date><risdate>2009</risdate><volume>124</volume><issue>5</issue><spage>1437</spage><epage>1446</epage><pages>1437-1446</pages><issn>0032-1052</issn><eissn>1529-4242</eissn><abstract>The efficacy of autologous fat transplantation is reduced by fat absorption and fibrosis due to fat necrosis. Enhanced transplant neovascularization early after transplantation may reduce these outcomes. The authors asked whether cell and concomitant gene therapy using adipose-derived stem cells transduced with vascular endothelial growth factor (VEGF) improves fat transplant neovascularization and survival.
Human adipose-derived stem cells were expanded ex vivo for three passages, labeled with 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine (DiI), and transduced with VEGF or left untransduced. Human fat tissues were then mixed with the DiI-labeled VEGF-transduced adipose-derived stem cells, the DiI-labeled adipose-derived stem cells, the known vascularization-promoting agent insulin, or medium alone, and 18 nude mice were injected subcutaneously with all four preparations, with each of the four designated spots receiving one of these four mixtures in a random fashion. Six months later, transplanted tissue volume and histology were evaluated and neovascularization was quantified by counting the capillaries.
Control transplant survival was 27.1 +/- 8.2 percent, but mixture with the VEGF-transduced and VEGF-untransduced stem cells significantly increased transplant survival (74.1 +/- 12.6 percent and 60.1 +/- 17.6 percent, respectively). Insulin was less effective (37.7 +/- 6.9 percent). Histological analysis revealed both types of transplants consisted predominantly of adipose tissue, unlike the control transplants, and had significantly less fat necrosis and fibrosis. The VEGF-transduced, adipose-derived stem cell-treated transplants had significantly higher capillary density than the other transplants and bore DiI-double-positive and CD31-double-positive cells (i.e., adipose-derived stem cell-derived endothelial cells).
Adipose-derived stem cells together with VEGF transduction can enhance the survival and quality of transplanted fat tissues.</abstract><cop>Hagerstown, MD</cop><pub>American Society of Plastic Surgeons</pub><pmid>20009828</pmid><doi>10.1097/PRS.0b013e3181babbb6</doi><tpages>10</tpages></addata></record> |
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| identifier | ISSN: 0032-1052 |
| ispartof | Plastic and reconstructive surgery (1963), 2009-11, Vol.124 (5), p.1437-1446 |
| issn | 0032-1052 1529-4242 |
| language | eng |
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| source | MEDLINE; Journals@Ovid Complete |
| subjects | Adenoviridae Adipocytes - transplantation Adipose Tissue - cytology Adipose Tissue - transplantation Animals Biological and medical sciences Gene Expression Regulation, Viral Graft Survival Humans Medical sciences Mice Mice, Nude Models, Animal Neovascularization, Physiologic - genetics Stem Cell Transplantation - methods Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Transduction, Genetic Transfection Transgenes Transplantation, Autologous Vascular Endothelial Growth Factor A - genetics |
| title | Improvement of the Survival of Human Autologous Fat Transplantation by Using VEGF-Transfected Adipose-Derived Stem Cells |
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