Immobilization of glucose oxidase on polymer membranes treated by low-temperature plasma

Low‐temperature plasma was employed for activation of polymer membranes as a carrier for enzyme immobilization. Glucose oxidase was immobilized on polypropylene (PP), polyvinylidene fluoride (PVDF), or polytetrafluoroethylene (PTFE) membrane surfaces treated by nitrogen or ammonia gas plasma using g...

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Veröffentlicht in:Biotechnology and bioengineering 1988-07, Vol.32 (3), p.369-373
Hauptverfasser: Kawakami, Mitsuyasu, Koya, Hidekazu, Gondo, Shinichiro
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container_title Biotechnology and bioengineering
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creator Kawakami, Mitsuyasu
Koya, Hidekazu
Gondo, Shinichiro
description Low‐temperature plasma was employed for activation of polymer membranes as a carrier for enzyme immobilization. Glucose oxidase was immobilized on polypropylene (PP), polyvinylidene fluoride (PVDF), or polytetrafluoroethylene (PTFE) membrane surfaces treated by nitrogen or ammonia gas plasma using glutaraldehyde as a linking agent. Enzyme activity was evaluated by the response of glucose sensor composed of the immobilized enzyme membrane and a dissolved oxygen electrode. The sensor response was found to depend on the kind of carrier membrane and to become maximum at suitable conditions of plasma treatment.
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source Wiley Online Library Journals Frontfile Complete
subjects Biological and medical sciences
Biotechnology
Enzyme engineering
Fundamental and applied biological sciences. Psychology
gases
glucose oxidase
immobilization
Immobilization of enzymes and other molecules
Immobilization techniques
immobilized enzymes
Methods. Procedures. Technologies
plasma
polypropylene
polytetrafluoroethylene
polyvinylidene fluoride
title Immobilization of glucose oxidase on polymer membranes treated by low-temperature plasma
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