A New Method to Prepare Multicellular Spheroids in Cancer Cell Lines Using a Thermo-Reversible Gelation Polymer

: The purpose of this study is to utilize the thermo‐reversible gelation polymer in which the sol‐gel transitting phase is reversibly changed by temperature in a three‐dimensional culture system. Human cancer cells have been observed to form multicellular spheroids, whereas fibroblasts slowly develo...

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Veröffentlicht in:	Artificial organs 2003-07, Vol.27 (7), p.598-604
Hauptverfasser:	Tsukikawa, Satoshi, Matsuoka, Hiromitsu, Kurahashi, Yuko, Konno, Yasushi, Satoh, Koh, Satoh, Ryotaroh, Isogai, Akiko, Kimura, Kanako, Watanabe, Yasuharu, Nakano, Suehiro, Hayashi, Junya, Kubota, Sunao
Format:	Artikel
Sprache:	eng
Schlagworte:	Cell Culture Techniques - methods Cell Division Cell Line, Tumor - cytology Culture Media Gels Humans Multicellular spheroids Polymers Spheroids, Cellular - cytology Temperature Thermo-reversible gelation polymer Three-dimensional culture
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