Effects of high glucose concentration on survival and respiration of human endothelial cells
To determine whether or not endothelial cell survival was decreased after incubation with high glucose concentrations in culture media, we studied the influence of d-glucose or l-glucose (a non-metabolizable stereoisomer of d-glucose) on cell survival using the trypan-blue exclusion test. Simultaneo...
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| Veröffentlicht in: | Biochimie 1992-11, Vol.74 (11), p.975-979 |
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| container_title | Biochimie |
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| creator | Foultier, M.T. Vonarx-Coinsmann, V. Patrice, T. Cloarec, D. Xu, X.N. Combre, A. |
| description | To determine whether or not endothelial cell survival was decreased after incubation with high glucose concentrations in culture media, we studied the influence of
d-glucose or
l-glucose (a non-metabolizable stereoisomer of
d-glucose) on cell survival using the trypan-blue exclusion test. Simultaneousl the MTT (3(4,5-dimethylthiazol-2-y)-2,5 diphenyl tetrazolium bromide) assay was used to measure both the mitochondrial respiratory chain activity and cell viability. Respiratory chain activity per cell increased when
D-glucose concentrations rose but at the same time trypan-blue excluded cells were decreased. Comparison with data in the literature showed that the MTT assay was not reliable for studies involving endothelial cell survival when glucose reduction was affected on these cells. It seems important to check MTT assay reliability carefully when it is used for drugs affecting glucose metabolism, or with other cell types. |
| doi_str_mv | 10.1016/0300-9084(92)90017-9 |
| format | Article |
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d-glucose or
l-glucose (a non-metabolizable stereoisomer of
d-glucose) on cell survival using the trypan-blue exclusion test. Simultaneousl the MTT (3(4,5-dimethylthiazol-2-y)-2,5 diphenyl tetrazolium bromide) assay was used to measure both the mitochondrial respiratory chain activity and cell viability. Respiratory chain activity per cell increased when
D-glucose concentrations rose but at the same time trypan-blue excluded cells were decreased. Comparison with data in the literature showed that the MTT assay was not reliable for studies involving endothelial cell survival when glucose reduction was affected on these cells. It seems important to check MTT assay reliability carefully when it is used for drugs affecting glucose metabolism, or with other cell types.</description><identifier>ISSN: 0300-9084</identifier><identifier>EISSN: 1638-6183</identifier><identifier>DOI: 10.1016/0300-9084(92)90017-9</identifier><identifier>PMID: 1477141</identifier><identifier>CODEN: BICMBE</identifier><language>eng</language><publisher>Paris: Elsevier Masson SAS</publisher><subject>Biological and medical sciences ; Cell structures and functions ; Cell Survival - drug effects ; Cells, Cultured ; Colorimetry ; Coloring Agents ; Culture Media ; Electron Transport - drug effects ; endothelial glucose ; Endothelium, Vascular - cytology ; Endothelium, Vascular - drug effects ; Fundamental and applied biological sciences. Psychology ; Glucose - pharmacology ; glucose metabolism ; Humans ; Mitochondria and cell respiration ; Molecular and cellular biology ; MTT assay ; Tetrazolium Salts ; Thiazoles ; Trypan Blue</subject><ispartof>Biochimie, 1992-11, Vol.74 (11), p.975-979</ispartof><rights>1992</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-b404dc837dbe03186266a6a7f9e7cd0b839cfba069d204f02e58c00553cea7383</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0300-9084(92)90017-9$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,45974</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4430868$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1477141$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Foultier, M.T.</creatorcontrib><creatorcontrib>Vonarx-Coinsmann, V.</creatorcontrib><creatorcontrib>Patrice, T.</creatorcontrib><creatorcontrib>Cloarec, D.</creatorcontrib><creatorcontrib>Xu, X.N.</creatorcontrib><creatorcontrib>Combre, A.</creatorcontrib><title>Effects of high glucose concentration on survival and respiration of human endothelial cells</title><title>Biochimie</title><addtitle>Biochimie</addtitle><description>To determine whether or not endothelial cell survival was decreased after incubation with high glucose concentrations in culture media, we studied the influence of
d-glucose or
l-glucose (a non-metabolizable stereoisomer of
d-glucose) on cell survival using the trypan-blue exclusion test. Simultaneousl the MTT (3(4,5-dimethylthiazol-2-y)-2,5 diphenyl tetrazolium bromide) assay was used to measure both the mitochondrial respiratory chain activity and cell viability. Respiratory chain activity per cell increased when
D-glucose concentrations rose but at the same time trypan-blue excluded cells were decreased. Comparison with data in the literature showed that the MTT assay was not reliable for studies involving endothelial cell survival when glucose reduction was affected on these cells. It seems important to check MTT assay reliability carefully when it is used for drugs affecting glucose metabolism, or with other cell types.</description><subject>Biological and medical sciences</subject><subject>Cell structures and functions</subject><subject>Cell Survival - drug effects</subject><subject>Cells, Cultured</subject><subject>Colorimetry</subject><subject>Coloring Agents</subject><subject>Culture Media</subject><subject>Electron Transport - drug effects</subject><subject>endothelial glucose</subject><subject>Endothelium, Vascular - cytology</subject><subject>Endothelium, Vascular - drug effects</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glucose - pharmacology</subject><subject>glucose metabolism</subject><subject>Humans</subject><subject>Mitochondria and cell respiration</subject><subject>Molecular and cellular biology</subject><subject>MTT assay</subject><subject>Tetrazolium Salts</subject><subject>Thiazoles</subject><subject>Trypan Blue</subject><issn>0300-9084</issn><issn>1638-6183</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kV1rFDEUhoNU6lr9BwpzUUQvRk8m2XzcFKS0VSh4o3dCyCQn3cjsZJvMLPjvm-mu7Z0QyMX7vIeTJ4S8o_CZAhVfgAG0GhT_qLtPGoDKVr8gKyqYagVV7ISsnpBX5HUpfwBgDZ0-JaeUS0k5XZHfVyGgm0qTQrOJd5vmbphdKti4NDocp2ynmMamnjLnfdzbobGjbzKWXfyX1ea8tWODo0_TBodYIYfDUN6Ql8EOBd8e7zPy6_rq5-W39vbHzffLr7etY0pMbc-Be6eY9D0Co0p0QlhhZdAonYdeMe1Cb0Fo3wEP0OFaufqUNXNoJVPsjHw4zN3ldD9jmcw2lmUDO2Kai5GMU9lpXkF-AF1OpWQMZpfj1ua_hoJZpJrFmFmMGd2ZR6lG19r74_y536J_Lh0s1vz8mNvi7BCyHV0sTxjnDJRY1rw4YFhd7CNmU1zEqtnHXP_A-BT_v8cDuQiTcQ</recordid><startdate>19921101</startdate><enddate>19921101</enddate><creator>Foultier, M.T.</creator><creator>Vonarx-Coinsmann, V.</creator><creator>Patrice, T.</creator><creator>Cloarec, D.</creator><creator>Xu, X.N.</creator><creator>Combre, A.</creator><general>Elsevier Masson SAS</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19921101</creationdate><title>Effects of high glucose concentration on survival and respiration of human endothelial cells</title><author>Foultier, M.T. ; Vonarx-Coinsmann, V. ; Patrice, T. ; Cloarec, D. ; Xu, X.N. ; Combre, A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-b404dc837dbe03186266a6a7f9e7cd0b839cfba069d204f02e58c00553cea7383</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Biological and medical sciences</topic><topic>Cell structures and functions</topic><topic>Cell Survival - drug effects</topic><topic>Cells, Cultured</topic><topic>Colorimetry</topic><topic>Coloring Agents</topic><topic>Culture Media</topic><topic>Electron Transport - drug effects</topic><topic>endothelial glucose</topic><topic>Endothelium, Vascular - cytology</topic><topic>Endothelium, Vascular - drug effects</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glucose - pharmacology</topic><topic>glucose metabolism</topic><topic>Humans</topic><topic>Mitochondria and cell respiration</topic><topic>Molecular and cellular biology</topic><topic>MTT assay</topic><topic>Tetrazolium Salts</topic><topic>Thiazoles</topic><topic>Trypan Blue</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Foultier, M.T.</creatorcontrib><creatorcontrib>Vonarx-Coinsmann, V.</creatorcontrib><creatorcontrib>Patrice, T.</creatorcontrib><creatorcontrib>Cloarec, D.</creatorcontrib><creatorcontrib>Xu, X.N.</creatorcontrib><creatorcontrib>Combre, A.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biochimie</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Foultier, M.T.</au><au>Vonarx-Coinsmann, V.</au><au>Patrice, T.</au><au>Cloarec, D.</au><au>Xu, X.N.</au><au>Combre, A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of high glucose concentration on survival and respiration of human endothelial cells</atitle><jtitle>Biochimie</jtitle><addtitle>Biochimie</addtitle><date>1992-11-01</date><risdate>1992</risdate><volume>74</volume><issue>11</issue><spage>975</spage><epage>979</epage><pages>975-979</pages><issn>0300-9084</issn><eissn>1638-6183</eissn><coden>BICMBE</coden><abstract>To determine whether or not endothelial cell survival was decreased after incubation with high glucose concentrations in culture media, we studied the influence of
d-glucose or
l-glucose (a non-metabolizable stereoisomer of
d-glucose) on cell survival using the trypan-blue exclusion test. Simultaneousl the MTT (3(4,5-dimethylthiazol-2-y)-2,5 diphenyl tetrazolium bromide) assay was used to measure both the mitochondrial respiratory chain activity and cell viability. Respiratory chain activity per cell increased when
D-glucose concentrations rose but at the same time trypan-blue excluded cells were decreased. Comparison with data in the literature showed that the MTT assay was not reliable for studies involving endothelial cell survival when glucose reduction was affected on these cells. It seems important to check MTT assay reliability carefully when it is used for drugs affecting glucose metabolism, or with other cell types.</abstract><cop>Paris</cop><pub>Elsevier Masson SAS</pub><pmid>1477141</pmid><doi>10.1016/0300-9084(92)90017-9</doi><tpages>5</tpages></addata></record> |
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| ispartof | Biochimie, 1992-11, Vol.74 (11), p.975-979 |
| issn | 0300-9084 1638-6183 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Biological and medical sciences Cell structures and functions Cell Survival - drug effects Cells, Cultured Colorimetry Coloring Agents Culture Media Electron Transport - drug effects endothelial glucose Endothelium, Vascular - cytology Endothelium, Vascular - drug effects Fundamental and applied biological sciences. Psychology Glucose - pharmacology glucose metabolism Humans Mitochondria and cell respiration Molecular and cellular biology MTT assay Tetrazolium Salts Thiazoles Trypan Blue |
| title | Effects of high glucose concentration on survival and respiration of human endothelial cells |
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