Janibacter hoylei sp. nov., Bacillus isronensis sp. nov. and Bacillus aryabhattai sp. nov., isolated from cryotubes used for collecting air from the upper atmosphere

1 Centre for Cellular and Molecular Biology, Uppal Road, Hyderabad 500 007, India 2 Microbial Type Culture Collection (MTCC), Institute of Microbial Technology, Sector 39A, Chandigarh 160 036, India 3 Microbial Culture Collection, National Centre for Cell Science, Pune University Campus, Ganeshkhind, Pune 411 007, India 4 ISRO Headquarters, Department of Space, Bangalore 560 023, India 5 Tata Institute of Fundamental Research, Homi Bhabha Road, Colaba, Mumbai 400 005, India 6 Anveshna, 12-13-100, Lane No. 1, Street No. 3, Tarnaka, Hyderabad 500 017, India 7 Inter-University Centre for Astronomy and Astrophysics, Ganeshkhind, Post Bag 4, Pune 411 007, India Correspondence S. Shivaji shivas{at}ccmb.res.in Three novel bacterial strains, PVAS-1 T , B3W22 T and B8W22 T , were isolated from cryotubes used to collect air samples at altitudes of between 27 and 41 km. Based on phenotypic characteristics, chemotaxonomic features, DNA–DNA hybridization with the nearest phylogenetic neighbours and phylogenetic analysis based on partial 16S rRNA gene sequences (PVAS-1 T , 1196 nt; B3W22 T , 1541 nt; B8W22 T , 1533 nt), the three strains were identified as representing novel species, and the names proposed are Janibacter hoylei sp. nov. (type strain PVAS-1 T =MTCC 8307 T =DSM 21601 T =CCUG 56714 T ), Bacillus isronensis sp. nov. (type strain B3W22 T =MTCC 7902 T =JCM 13838 T ) and Bacillus aryabhattai sp. nov. (type strain B8W22 T =MTCC 7755 T =JCM 13839 T ). The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains PVAS-1 T , B3W22 T and B8W22 T are DQ317608 , EF114311 and EF114313 . Polar lipid profiles of the novel strains and related type strains and results of UV-sensitivity experiments are available as supplementary material with the online version of this paper.