Rapid detection of Acanthamoeba cysts in frozen sections of corneal scrapings with Fungiflora Y
Aims:To evaluate the usefulness of serial frozen sections of corneal scrapings stained with Fungiflora Y (FFY) to diagnose Acanthamoeba keratitis (AK).Methods:Eight patients with suspected AK were studied. Serial frozen sections were made from part of the corneal epithelial scrapings and stained wit...
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| Veröffentlicht in: | British journal of ophthalmology 2009-12, Vol.93 (12), p.1563-1565 |
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| creator | Shiraishi, A Kobayashi, T Hara, Y Yamaguchi, M Uno, T Ohashi, Y |
| description | Aims:To evaluate the usefulness of serial frozen sections of corneal scrapings stained with Fungiflora Y (FFY) to diagnose Acanthamoeba keratitis (AK).Methods:Eight patients with suspected AK were studied. Serial frozen sections were made from part of the corneal epithelial scrapings and stained with FFY. The remaining corneal epithelial scrapings were submitted for laboratory culture.Results:The FFY stained frozen sections were completed within an hour, and Acanthamoeba cysts were detected under a fluorescence microscope in all eight patients. The same sections were examined with a light microscope, and Acanthamoeba cysts were confirmed to be present from their morphological characteristics. Five of the eight patients had positive laboratory cultures for Acanthamoeba.Conclusion:FFY staining of frozen sections of corneal scrapings is a rapid and reliable technique which can be used to make an early diagnosis of AK. |
| doi_str_mv | 10.1136/bjo.2009.158113 |
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Serial frozen sections were made from part of the corneal epithelial scrapings and stained with FFY. The remaining corneal epithelial scrapings were submitted for laboratory culture.Results:The FFY stained frozen sections were completed within an hour, and Acanthamoeba cysts were detected under a fluorescence microscope in all eight patients. The same sections were examined with a light microscope, and Acanthamoeba cysts were confirmed to be present from their morphological characteristics. Five of the eight patients had positive laboratory cultures for Acanthamoeba.Conclusion:FFY staining of frozen sections of corneal scrapings is a rapid and reliable technique which can be used to make an early diagnosis of AK.</description><identifier>ISSN: 0007-1161</identifier><identifier>EISSN: 1468-2079</identifier><identifier>DOI: 10.1136/bjo.2009.158113</identifier><identifier>PMID: 19939794</identifier><identifier>CODEN: BJOPAL</identifier><language>eng</language><publisher>BMA House, Tavistock Square, London, WC1H 9JR: BMJ Publishing Group Ltd</publisher><subject>Acanthamoeba - isolation & purification ; Acanthamoeba Keratitis - diagnosis ; Acanthamoeba Keratitis - etiology ; Adolescent ; Adult ; Biological and medical sciences ; Cellular biology ; Cellulose ; Contact lenses ; Contact Lenses, Hydrophilic - adverse effects ; Cysts ; Early Diagnosis ; Epithelium, Corneal - microbiology ; Female ; Fluorescent Dyes ; Frozen Sections ; Fungi ; Humans ; Male ; Medical sciences ; Microscopy, Fluorescence ; Miscellaneous ; Morphology ; Ophthalmology ; Organic Chemicals ; Staining and Labeling - methods ; Young Adult</subject><ispartof>British journal of ophthalmology, 2009-12, Vol.93 (12), p.1563-1565</ispartof><rights>BMJ Publishing Group Ltd. All rights reserved.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright: 2009 BMJ Publishing Group Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b427t-31249dd1aa990b8dfa3812f0a9ffea7942cea6e059d6f7b316059b6fa41e75193</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttp://bjo.bmj.com/content/93/12/1563.full.pdf$$EPDF$$P50$$Gbmj$$H</linktopdf><linktohtml>$$Uhttp://bjo.bmj.com/content/93/12/1563.full$$EHTML$$P50$$Gbmj$$H</linktohtml><link.rule.ids>114,115,314,780,784,3195,23570,27923,27924,77471,77502</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22154222$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19939794$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shiraishi, A</creatorcontrib><creatorcontrib>Kobayashi, T</creatorcontrib><creatorcontrib>Hara, Y</creatorcontrib><creatorcontrib>Yamaguchi, M</creatorcontrib><creatorcontrib>Uno, T</creatorcontrib><creatorcontrib>Ohashi, Y</creatorcontrib><title>Rapid detection of Acanthamoeba cysts in frozen sections of corneal scrapings with Fungiflora Y</title><title>British journal of ophthalmology</title><addtitle>Br J Ophthalmol</addtitle><description>Aims:To evaluate the usefulness of serial frozen sections of corneal scrapings stained with Fungiflora Y (FFY) to diagnose Acanthamoeba keratitis (AK).Methods:Eight patients with suspected AK were studied. Serial frozen sections were made from part of the corneal epithelial scrapings and stained with FFY. The remaining corneal epithelial scrapings were submitted for laboratory culture.Results:The FFY stained frozen sections were completed within an hour, and Acanthamoeba cysts were detected under a fluorescence microscope in all eight patients. The same sections were examined with a light microscope, and Acanthamoeba cysts were confirmed to be present from their morphological characteristics. Five of the eight patients had positive laboratory cultures for Acanthamoeba.Conclusion:FFY staining of frozen sections of corneal scrapings is a rapid and reliable technique which can be used to make an early diagnosis of AK.</description><subject>Acanthamoeba - isolation & purification</subject><subject>Acanthamoeba Keratitis - diagnosis</subject><subject>Acanthamoeba Keratitis - etiology</subject><subject>Adolescent</subject><subject>Adult</subject><subject>Biological and medical sciences</subject><subject>Cellular biology</subject><subject>Cellulose</subject><subject>Contact lenses</subject><subject>Contact Lenses, Hydrophilic - adverse effects</subject><subject>Cysts</subject><subject>Early Diagnosis</subject><subject>Epithelium, Corneal - microbiology</subject><subject>Female</subject><subject>Fluorescent Dyes</subject><subject>Frozen Sections</subject><subject>Fungi</subject><subject>Humans</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Microscopy, Fluorescence</subject><subject>Miscellaneous</subject><subject>Morphology</subject><subject>Ophthalmology</subject><subject>Organic Chemicals</subject><subject>Staining and Labeling - methods</subject><subject>Young Adult</subject><issn>0007-1161</issn><issn>1468-2079</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><recordid>eNqF0M1rFDEYBvAgil2rZ28SEBGE2ebNzCSTY1msCquCHwV7Ce9kknbW2WSbzNDWv96ss1Tw4ilfvzy8PIQ8B7YEKMVJuwlLzphaQt3kiwdkAZVoCs6kekgWjDFZAAg4Ik9S2uQjFyAfkyNQqlRSVQuiv-Cu72hnR2vGPngaHD016Mcr3AbbIjV3aUy099TF8Mt6mmaX9tCE6C0ONJmYU_xlojf9eEXPJn_ZuyFEpD-ekkcOh2SfHdZj8v3s7bfV-2L9-d2H1em6aCsux6IEXqmuA0SlWNt0DssGuGOonLOYJ-XGorCsVp1wsi1B5G0rHFZgZQ2qPCav59xdDNeTTaPe9snYYUBvw5S0LKs_FVVZvvxHbsIUfR5Og5SNKkEJyOpkViaGlKJ1ehf7LcY7DUzvq9e5er2vXs_V5x8vDrlTu7XdX3_oOoNXB4DJ4OAietOne8c51BXnPLtidn0a7e39O8afWshS1vrT-Uqzrxdw_nF9oVfZv5l9u938d8rfNrWoJQ</recordid><startdate>20091201</startdate><enddate>20091201</enddate><creator>Shiraishi, A</creator><creator>Kobayashi, T</creator><creator>Hara, Y</creator><creator>Yamaguchi, M</creator><creator>Uno, T</creator><creator>Ohashi, Y</creator><general>BMJ Publishing Group Ltd</general><general>BMJ Publishing Group</general><general>BMJ Publishing Group LTD</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>BTHHO</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20091201</creationdate><title>Rapid detection of Acanthamoeba cysts in frozen sections of corneal scrapings with Fungiflora Y</title><author>Shiraishi, A ; Kobayashi, T ; Hara, Y ; Yamaguchi, M ; Uno, T ; Ohashi, Y</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b427t-31249dd1aa990b8dfa3812f0a9ffea7942cea6e059d6f7b316059b6fa41e75193</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Acanthamoeba - isolation & purification</topic><topic>Acanthamoeba Keratitis - diagnosis</topic><topic>Acanthamoeba Keratitis - etiology</topic><topic>Adolescent</topic><topic>Adult</topic><topic>Biological and medical sciences</topic><topic>Cellular biology</topic><topic>Cellulose</topic><topic>Contact lenses</topic><topic>Contact Lenses, Hydrophilic - adverse effects</topic><topic>Cysts</topic><topic>Early Diagnosis</topic><topic>Epithelium, Corneal - microbiology</topic><topic>Female</topic><topic>Fluorescent Dyes</topic><topic>Frozen Sections</topic><topic>Fungi</topic><topic>Humans</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Microscopy, Fluorescence</topic><topic>Miscellaneous</topic><topic>Morphology</topic><topic>Ophthalmology</topic><topic>Organic Chemicals</topic><topic>Staining and Labeling - methods</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shiraishi, A</creatorcontrib><creatorcontrib>Kobayashi, T</creatorcontrib><creatorcontrib>Hara, Y</creatorcontrib><creatorcontrib>Yamaguchi, M</creatorcontrib><creatorcontrib>Uno, T</creatorcontrib><creatorcontrib>Ohashi, Y</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central</collection><collection>BMJ Journals</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>British journal of ophthalmology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shiraishi, A</au><au>Kobayashi, T</au><au>Hara, Y</au><au>Yamaguchi, M</au><au>Uno, T</au><au>Ohashi, Y</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rapid detection of Acanthamoeba cysts in frozen sections of corneal scrapings with Fungiflora Y</atitle><jtitle>British journal of ophthalmology</jtitle><addtitle>Br J Ophthalmol</addtitle><date>2009-12-01</date><risdate>2009</risdate><volume>93</volume><issue>12</issue><spage>1563</spage><epage>1565</epage><pages>1563-1565</pages><issn>0007-1161</issn><eissn>1468-2079</eissn><coden>BJOPAL</coden><abstract>Aims:To evaluate the usefulness of serial frozen sections of corneal scrapings stained with Fungiflora Y (FFY) to diagnose Acanthamoeba keratitis (AK).Methods:Eight patients with suspected AK were studied. Serial frozen sections were made from part of the corneal epithelial scrapings and stained with FFY. The remaining corneal epithelial scrapings were submitted for laboratory culture.Results:The FFY stained frozen sections were completed within an hour, and Acanthamoeba cysts were detected under a fluorescence microscope in all eight patients. The same sections were examined with a light microscope, and Acanthamoeba cysts were confirmed to be present from their morphological characteristics. Five of the eight patients had positive laboratory cultures for Acanthamoeba.Conclusion:FFY staining of frozen sections of corneal scrapings is a rapid and reliable technique which can be used to make an early diagnosis of AK.</abstract><cop>BMA House, Tavistock Square, London, WC1H 9JR</cop><pub>BMJ Publishing Group Ltd</pub><pmid>19939794</pmid><doi>10.1136/bjo.2009.158113</doi><tpages>3</tpages></addata></record> |
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| subjects | Acanthamoeba - isolation & purification Acanthamoeba Keratitis - diagnosis Acanthamoeba Keratitis - etiology Adolescent Adult Biological and medical sciences Cellular biology Cellulose Contact lenses Contact Lenses, Hydrophilic - adverse effects Cysts Early Diagnosis Epithelium, Corneal - microbiology Female Fluorescent Dyes Frozen Sections Fungi Humans Male Medical sciences Microscopy, Fluorescence Miscellaneous Morphology Ophthalmology Organic Chemicals Staining and Labeling - methods Young Adult |
| title | Rapid detection of Acanthamoeba cysts in frozen sections of corneal scrapings with Fungiflora Y |
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