Coencapsulation of Hepatocytes With Bone Marrow Mesenchymal Stem Cells Improves Hepatocyte-Specific Functions
Hepatocyte transplantation is an alternative to liver transplantation, which is hampered by short survival time and immunorejection. In this study, we investigated the specific functions of hepatocytes coencapsulated with bone marrow mesenchymal stem cells (MSCs) in vitro, and we further examined wh...
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| Veröffentlicht in: | Transplantation 2009-11, Vol.88 (10), p.1178-1185 |
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| creator | SHI, Xiao-Lei YUE ZHANG GU, Jin-Yang DING, Yi-Tao |
| description | Hepatocyte transplantation is an alternative to liver transplantation, which is hampered by short survival time and immunorejection. In this study, we investigated the specific functions of hepatocytes coencapsulated with bone marrow mesenchymal stem cells (MSCs) in vitro, and we further examined whether transplantation of coencapsulated hepatocytes and MSCs could enhance the ability of hepatocytes to alleviate acute liver failure in vivo.
Rat hepatocytes and MSCs were isolated and coencapsulated by alginate-poly-l-lysine-alginate microencapsulation. Cell functions were monitored during the course of cell culturing. Acute liver failure in rats was induced by d-galactosamine administration. These rats were subjected to intraperitoneal transplantation of coencapsulated hepatocytes with MSCs, encapsulated hepatocytes alone, or empty vehicles after 24 hr. The survival rate and liver functions were assessed. Immunofluorescence microscopy was used for the analysis of coencapsulated cells before and 7 days after transplantation.
Hepatocyte-specific functions, including albumin secretion and urea synthesis, were all significantly improved in the coencapsulation group compared with the encapsulated hepatocytes group (P |
| doi_str_mv | 10.1097/TP.0b013e3181bc288b |
| format | Article |
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Rat hepatocytes and MSCs were isolated and coencapsulated by alginate-poly-l-lysine-alginate microencapsulation. Cell functions were monitored during the course of cell culturing. Acute liver failure in rats was induced by d-galactosamine administration. These rats were subjected to intraperitoneal transplantation of coencapsulated hepatocytes with MSCs, encapsulated hepatocytes alone, or empty vehicles after 24 hr. The survival rate and liver functions were assessed. Immunofluorescence microscopy was used for the analysis of coencapsulated cells before and 7 days after transplantation.
Hepatocyte-specific functions, including albumin secretion and urea synthesis, were all significantly improved in the coencapsulation group compared with the encapsulated hepatocytes group (P<0.05). Similar trend was observed for cell cycle analysis of hepatocytes. Intraperitoneal transplantation of coencapsulated hepatocytes with MSCs not only increased the survival rate but also improved liver functions in a rat model of acute liver failure. Some MSCs transdifferentiated into hepatocyte-like cells in vivo, which expressed albumin, a typical marker of hepatocyte.
Encapsulation of hepatocytes and MSCs improved hepatocyte-specific functions in vitro and in vivo. Transplantation of coencapsulated hepatocytes and MSC might be a promising strategy for cell-based therapy for acute liver diseases.</description><identifier>ISSN: 0041-1337</identifier><identifier>EISSN: 1534-6080</identifier><identifier>DOI: 10.1097/TP.0b013e3181bc288b</identifier><identifier>PMID: 19935371</identifier><identifier>CODEN: TRPLAU</identifier><language>eng</language><publisher>Hagerstown, MD: Lippincott Williams & Wilkins</publisher><subject>Acute Disease ; Animals ; Biological and medical sciences ; Bone Marrow Cells - cytology ; Bone Marrow Cells - physiology ; Cell Culture Techniques - methods ; Cell Cycle - physiology ; Disease Models, Animal ; Femur ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Hepatocytes - cytology ; Hepatocytes - pathology ; Hepatocytes - physiology ; Hepatocytes - transplantation ; Liver Failure - mortality ; Liver Failure - pathology ; Liver Failure - surgery ; Liver Function Tests ; Medical sciences ; Mesenchymal Stromal Cells - cytology ; Mesenchymal Stromal Cells - physiology ; Rats ; Rats, Sprague-Dawley ; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases ; Survival Rate ; Survivors ; Tissue, organ and graft immunology</subject><ispartof>Transplantation, 2009-11, Vol.88 (10), p.1178-1185</ispartof><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c379t-920662a538db2f05723666b7ac28e182251f65a7ebe7d498ae65ae3bb93a76b63</citedby><cites>FETCH-LOGICAL-c379t-920662a538db2f05723666b7ac28e182251f65a7ebe7d498ae65ae3bb93a76b63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22176043$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19935371$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>SHI, Xiao-Lei</creatorcontrib><creatorcontrib>YUE ZHANG</creatorcontrib><creatorcontrib>GU, Jin-Yang</creatorcontrib><creatorcontrib>DING, Yi-Tao</creatorcontrib><title>Coencapsulation of Hepatocytes With Bone Marrow Mesenchymal Stem Cells Improves Hepatocyte-Specific Functions</title><title>Transplantation</title><addtitle>Transplantation</addtitle><description>Hepatocyte transplantation is an alternative to liver transplantation, which is hampered by short survival time and immunorejection. In this study, we investigated the specific functions of hepatocytes coencapsulated with bone marrow mesenchymal stem cells (MSCs) in vitro, and we further examined whether transplantation of coencapsulated hepatocytes and MSCs could enhance the ability of hepatocytes to alleviate acute liver failure in vivo.
Rat hepatocytes and MSCs were isolated and coencapsulated by alginate-poly-l-lysine-alginate microencapsulation. Cell functions were monitored during the course of cell culturing. Acute liver failure in rats was induced by d-galactosamine administration. These rats were subjected to intraperitoneal transplantation of coencapsulated hepatocytes with MSCs, encapsulated hepatocytes alone, or empty vehicles after 24 hr. The survival rate and liver functions were assessed. Immunofluorescence microscopy was used for the analysis of coencapsulated cells before and 7 days after transplantation.
Hepatocyte-specific functions, including albumin secretion and urea synthesis, were all significantly improved in the coencapsulation group compared with the encapsulated hepatocytes group (P<0.05). Similar trend was observed for cell cycle analysis of hepatocytes. Intraperitoneal transplantation of coencapsulated hepatocytes with MSCs not only increased the survival rate but also improved liver functions in a rat model of acute liver failure. Some MSCs transdifferentiated into hepatocyte-like cells in vivo, which expressed albumin, a typical marker of hepatocyte.
Encapsulation of hepatocytes and MSCs improved hepatocyte-specific functions in vitro and in vivo. Transplantation of coencapsulated hepatocytes and MSC might be a promising strategy for cell-based therapy for acute liver diseases.</description><subject>Acute Disease</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Bone Marrow Cells - cytology</subject><subject>Bone Marrow Cells - physiology</subject><subject>Cell Culture Techniques - methods</subject><subject>Cell Cycle - physiology</subject><subject>Disease Models, Animal</subject><subject>Femur</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Hepatocytes - cytology</subject><subject>Hepatocytes - pathology</subject><subject>Hepatocytes - physiology</subject><subject>Hepatocytes - transplantation</subject><subject>Liver Failure - mortality</subject><subject>Liver Failure - pathology</subject><subject>Liver Failure - surgery</subject><subject>Liver Function Tests</subject><subject>Medical sciences</subject><subject>Mesenchymal Stromal Cells - cytology</subject><subject>Mesenchymal Stromal Cells - physiology</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</subject><subject>Survival Rate</subject><subject>Survivors</subject><subject>Tissue, organ and graft immunology</subject><issn>0041-1337</issn><issn>1534-6080</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkE1LxDAQhoMoun78AkFyEU_VJNMk7VEXdYUVBVc8liQ7xUrb1KZV9t-bxcUFT5OB53mZvISccnbJWa6vFs-XzDIOCDzj1okssztkwiWkiWIZ2yUTxlKecAB9QA5D-GCMSdB6nxzwPIf45BPSTD22znRhrM1Q-Zb6ks6wM4N3qwEDfauGd3rjW6SPpu_9N33EEIX3VWNq-jJgQ6dY14E-NF3vv6KwlZOXDl1VVo7eja1bh4djsleaOuDJZh6R17vbxXSWzJ_uH6bX88SBzockF0wpYSRkSytKJrUApZTVJn4SeSaE5KWSRqNFvUzzzGDcEKzNwWhlFRyRi9_ceNTniGEomiq4eKhp0Y-h0JByKSXTkYRf0vU-hB7LouurxvSrgrNiXXOxeC7-1xyts03-aBtcbp1NrxE43wAmOFOXvWldFf44IbhWLAX4AaJLh9s</recordid><startdate>20091127</startdate><enddate>20091127</enddate><creator>SHI, Xiao-Lei</creator><creator>YUE ZHANG</creator><creator>GU, Jin-Yang</creator><creator>DING, Yi-Tao</creator><general>Lippincott Williams & Wilkins</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20091127</creationdate><title>Coencapsulation of Hepatocytes With Bone Marrow Mesenchymal Stem Cells Improves Hepatocyte-Specific Functions</title><author>SHI, Xiao-Lei ; YUE ZHANG ; GU, Jin-Yang ; DING, Yi-Tao</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c379t-920662a538db2f05723666b7ac28e182251f65a7ebe7d498ae65ae3bb93a76b63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Acute Disease</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Bone Marrow Cells - cytology</topic><topic>Bone Marrow Cells - physiology</topic><topic>Cell Culture Techniques - methods</topic><topic>Cell Cycle - physiology</topic><topic>Disease Models, Animal</topic><topic>Femur</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Hepatocytes - cytology</topic><topic>Hepatocytes - pathology</topic><topic>Hepatocytes - physiology</topic><topic>Hepatocytes - transplantation</topic><topic>Liver Failure - mortality</topic><topic>Liver Failure - pathology</topic><topic>Liver Failure - surgery</topic><topic>Liver Function Tests</topic><topic>Medical sciences</topic><topic>Mesenchymal Stromal Cells - cytology</topic><topic>Mesenchymal Stromal Cells - physiology</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases</topic><topic>Survival Rate</topic><topic>Survivors</topic><topic>Tissue, organ and graft immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>SHI, Xiao-Lei</creatorcontrib><creatorcontrib>YUE ZHANG</creatorcontrib><creatorcontrib>GU, Jin-Yang</creatorcontrib><creatorcontrib>DING, Yi-Tao</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Transplantation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>SHI, Xiao-Lei</au><au>YUE ZHANG</au><au>GU, Jin-Yang</au><au>DING, Yi-Tao</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Coencapsulation of Hepatocytes With Bone Marrow Mesenchymal Stem Cells Improves Hepatocyte-Specific Functions</atitle><jtitle>Transplantation</jtitle><addtitle>Transplantation</addtitle><date>2009-11-27</date><risdate>2009</risdate><volume>88</volume><issue>10</issue><spage>1178</spage><epage>1185</epage><pages>1178-1185</pages><issn>0041-1337</issn><eissn>1534-6080</eissn><coden>TRPLAU</coden><abstract>Hepatocyte transplantation is an alternative to liver transplantation, which is hampered by short survival time and immunorejection. In this study, we investigated the specific functions of hepatocytes coencapsulated with bone marrow mesenchymal stem cells (MSCs) in vitro, and we further examined whether transplantation of coencapsulated hepatocytes and MSCs could enhance the ability of hepatocytes to alleviate acute liver failure in vivo.
Rat hepatocytes and MSCs were isolated and coencapsulated by alginate-poly-l-lysine-alginate microencapsulation. Cell functions were monitored during the course of cell culturing. Acute liver failure in rats was induced by d-galactosamine administration. These rats were subjected to intraperitoneal transplantation of coencapsulated hepatocytes with MSCs, encapsulated hepatocytes alone, or empty vehicles after 24 hr. The survival rate and liver functions were assessed. Immunofluorescence microscopy was used for the analysis of coencapsulated cells before and 7 days after transplantation.
Hepatocyte-specific functions, including albumin secretion and urea synthesis, were all significantly improved in the coencapsulation group compared with the encapsulated hepatocytes group (P<0.05). Similar trend was observed for cell cycle analysis of hepatocytes. Intraperitoneal transplantation of coencapsulated hepatocytes with MSCs not only increased the survival rate but also improved liver functions in a rat model of acute liver failure. Some MSCs transdifferentiated into hepatocyte-like cells in vivo, which expressed albumin, a typical marker of hepatocyte.
Encapsulation of hepatocytes and MSCs improved hepatocyte-specific functions in vitro and in vivo. Transplantation of coencapsulated hepatocytes and MSC might be a promising strategy for cell-based therapy for acute liver diseases.</abstract><cop>Hagerstown, MD</cop><pub>Lippincott Williams & Wilkins</pub><pmid>19935371</pmid><doi>10.1097/TP.0b013e3181bc288b</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Acute Disease Animals Biological and medical sciences Bone Marrow Cells - cytology Bone Marrow Cells - physiology Cell Culture Techniques - methods Cell Cycle - physiology Disease Models, Animal Femur Fundamental and applied biological sciences. Psychology Fundamental immunology Hepatocytes - cytology Hepatocytes - pathology Hepatocytes - physiology Hepatocytes - transplantation Liver Failure - mortality Liver Failure - pathology Liver Failure - surgery Liver Function Tests Medical sciences Mesenchymal Stromal Cells - cytology Mesenchymal Stromal Cells - physiology Rats Rats, Sprague-Dawley Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Survival Rate Survivors Tissue, organ and graft immunology |
| title | Coencapsulation of Hepatocytes With Bone Marrow Mesenchymal Stem Cells Improves Hepatocyte-Specific Functions |
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