Chrysanthemum morifolium Ramat (CM) extract protects human neuroblastoma SH-SY5Y cells against MPP +-induced cytotoxicity

Chrysanthemum morifolium Ramat (CM) extract exhibited remarkable neuroprotective activity against MPP +-induced cytotoxicity in human neuroblastoma SH-SY5Y cells. CM also attenuated the elevation of ROS level, increase in Bax/Bcl-2 ratio, cleavage of caspase-3 and PARP proteolysis. Basing on its use as a traditional herbal medicine for several centuries and with the present scientific study CM might be a potential candidate in neurodegenerative diseases such as Parkinson's disease. Chrysanthemum morifolium Ramat (Asteraceae) has (CM) long been used in Korean and Chinese traditional herbal medicines with numerous therapeutic applications. To evaluate the neuroprotective activities of Chrysanthemum morifolium (CM) extract against 1-methyl-4-phenylpridinium ions (MPP +), Parkinsonian toxin through oxidative stress and impaired energy metabolism, in human SH-SY5Y neuroblastoma cells and the underlying mechanisms. The effects of CM against MPP +-induced cytotoxicity and neuronal cell viability, oxidative damage, the expression of Bcl-2 and Bax, caspase-3 and poly(ADP-ribose) polymerase (PARP) proteolysis were evaluated by using SH-SY5Y neuroblastoma cells. CM effectively inhibited the cytotoxicity and improved cell viability. CM also attenuated the elevation of reactive oxygen species (ROS) level, increase in Bax/Bcl-2 ratio, cleavage of caspase-3 and PARP proteolysis. These results demonstrate that CM possesses potent neuroprotective activity and therefore, might be a potential candidate in neurodegenerative diseases such as Parkinson's disease.