Identification of Plant Sphingolipid Desaturases Using Chromatography and Mass Spectrometry

This manuscript describes an efficient analytical assay combining high-performance liquid chromatography with UV detection (HPLC-UV), liquid chromatography coupled with tandem mass spectrometry (LC-MS-MS), and gas chromatography with mass spectrometry (GC-MS) for the characterization and C=C bond lo...

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Veröffentlicht in:	Journal of chromatographic science 2009-11, Vol.47 (10), p.895-901
Hauptverfasser:	Sun, Dayong, Froman, Byron E., Orth, Robert G., MacIsaac, Susan A., Larosa, Thomas, Dong, Fenggao, Valentin, Henry E.
Format:	Artikel
Sprache:	eng
Schlagworte:	Chromatography, High Pressure Liquid - methods Oxidoreductases - chemistry Oxidoreductases - genetics Oxidoreductases - metabolism Plant Proteins - chemistry Plant Proteins - genetics Plant Proteins - metabolism Primula - enzymology Recombinant Proteins - chemistry Recombinant Proteins - genetics Recombinant Proteins - metabolism Tandem Mass Spectrometry - methods
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