Biosensor-controlled degradation of chlorpyrifos and chlorfenvinfos using a phosphotriesterase-based detoxification column
This works presents the development of a detoxification system based on bacterial phosphotriesterase (PTE) for the degradation of organophosphate (OP) insecticides in water. PTE was immobilised on an activated agarose gel via covalent coupling. Two different OPs were studied, chlorpyrifos and chlorf...
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| Veröffentlicht in: | Chemosphere (Oxford) 2010, Vol.78 (1), p.1-6 |
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| creator | Istamboulie, Georges Durbiano, Romain Fournier, Didier Marty, Jean-Louis Noguer, Thierry |
| description | This works presents the development of a detoxification system based on bacterial phosphotriesterase (PTE) for the degradation of organophosphate (OP) insecticides in water. PTE was immobilised on an activated agarose gel via covalent coupling. Two different OPs were studied, chlorpyrifos and chlorfenvinfos, due to their importance in the field of water policy. The efficiency of insecticide degradation was controlled using a highly sensitive biosensor allowing the detection of OP concentration as low as 0.004
μg
L
−1. Under optimum conditions, it was shown that a column incorporating 500
IU of PTE was suitable for the detoxification of solutions containing either isolated pesticides or pesticides mixtures, even at concentrations higher than authorized limits. Finally, the method was shown to be adapted to the decontamination of real samples of pesticides with concentrations up to 20
μg
L
−1. |
| doi_str_mv | 10.1016/j.chemosphere.2009.10.037 |
| format | Article |
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μg
L
−1. Under optimum conditions, it was shown that a column incorporating 500
IU of PTE was suitable for the detoxification of solutions containing either isolated pesticides or pesticides mixtures, even at concentrations higher than authorized limits. Finally, the method was shown to be adapted to the decontamination of real samples of pesticides with concentrations up to 20
μg
L
−1.</description><identifier>ISSN: 0045-6535</identifier><identifier>EISSN: 1879-1298</identifier><identifier>DOI: 10.1016/j.chemosphere.2009.10.037</identifier><identifier>PMID: 19906400</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Acetylcholinesterase ; Bacteria ; Biosensing Techniques - methods ; Biosensor ; Chlorfenvinphos - analysis ; Chlorfenvinphos - metabolism ; Chlorpyrifos ; Chlorpyrifos - analysis ; Chlorpyrifos - metabolism ; Covalence ; Decontamination ; Degradation ; Enzymes, Immobilized - metabolism ; Insecticides ; Insecticides - analysis ; Insecticides - metabolism ; Organophosphates ; Pesticides ; Phosphoric Triester Hydrolases - metabolism ; Phosphotriesterase ; Water Pollutants, Chemical - analysis ; Water Pollutants, Chemical - metabolism</subject><ispartof>Chemosphere (Oxford), 2010, Vol.78 (1), p.1-6</ispartof><rights>2009 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c471t-592f44406b4a4a3d06e23c261d1df566f163f6fb08ffd5faf84036be918cf7723</citedby><cites>FETCH-LOGICAL-c471t-592f44406b4a4a3d06e23c261d1df566f163f6fb08ffd5faf84036be918cf7723</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0045653509012466$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,4009,27902,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19906400$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Istamboulie, Georges</creatorcontrib><creatorcontrib>Durbiano, Romain</creatorcontrib><creatorcontrib>Fournier, Didier</creatorcontrib><creatorcontrib>Marty, Jean-Louis</creatorcontrib><creatorcontrib>Noguer, Thierry</creatorcontrib><title>Biosensor-controlled degradation of chlorpyrifos and chlorfenvinfos using a phosphotriesterase-based detoxification column</title><title>Chemosphere (Oxford)</title><addtitle>Chemosphere</addtitle><description>This works presents the development of a detoxification system based on bacterial phosphotriesterase (PTE) for the degradation of organophosphate (OP) insecticides in water. PTE was immobilised on an activated agarose gel via covalent coupling. Two different OPs were studied, chlorpyrifos and chlorfenvinfos, due to their importance in the field of water policy. The efficiency of insecticide degradation was controlled using a highly sensitive biosensor allowing the detection of OP concentration as low as 0.004
μg
L
−1. Under optimum conditions, it was shown that a column incorporating 500
IU of PTE was suitable for the detoxification of solutions containing either isolated pesticides or pesticides mixtures, even at concentrations higher than authorized limits. Finally, the method was shown to be adapted to the decontamination of real samples of pesticides with concentrations up to 20
μg
L
−1.</description><subject>Acetylcholinesterase</subject><subject>Bacteria</subject><subject>Biosensing Techniques - methods</subject><subject>Biosensor</subject><subject>Chlorfenvinphos - analysis</subject><subject>Chlorfenvinphos - metabolism</subject><subject>Chlorpyrifos</subject><subject>Chlorpyrifos - analysis</subject><subject>Chlorpyrifos - metabolism</subject><subject>Covalence</subject><subject>Decontamination</subject><subject>Degradation</subject><subject>Enzymes, Immobilized - metabolism</subject><subject>Insecticides</subject><subject>Insecticides - analysis</subject><subject>Insecticides - metabolism</subject><subject>Organophosphates</subject><subject>Pesticides</subject><subject>Phosphoric Triester Hydrolases - metabolism</subject><subject>Phosphotriesterase</subject><subject>Water Pollutants, Chemical - analysis</subject><subject>Water Pollutants, Chemical - metabolism</subject><issn>0045-6535</issn><issn>1879-1298</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1u1DAUhSMEokPhFVDYQDcZfB3Hjpd0xJ9UiQ2sI8e-7niU2MFOqpanx1FGglVhY0vH3zn3yqco3gDZAwH-_rTXRxxDmo4YcU8JkVnfk1o8KXbQClkBle3TYkcIayre1M1F8SKlEyHZ3MjnxQVISTgjZFf8unYhoU8hVjr4OYZhQFMavI3KqNkFXwZb6uMQ4vQQnQ2pVN5sgkV_5_wqLcn521KV03HdKczRYZoxqoRVn481bw73zjq9ReowLKN_WTyzakj46nxfFj8-ffx--FLdfPv89fDhptJMwFw1klrGGOE9U0zVhnCktaYcDBjbcG6B15bbnrTWmsYq2zJS8x4ltNoKQevL4t2WO8Xwc8mbdaNLGodBeQxL6kTNoAHKRCbfPkrWDWmpkPBPkAIAkbCCV4-CIIQASriQGZUbqmNIKaLtpuhGFR86IN1ae3fq_qq9W2tfn3Lt2fv6PGbpRzR_nOeeM3DYAMwffecwdkk79BqNi6jnzgT3H2N-A1Ikx4s</recordid><startdate>2010</startdate><enddate>2010</enddate><creator>Istamboulie, Georges</creator><creator>Durbiano, Romain</creator><creator>Fournier, Didier</creator><creator>Marty, Jean-Louis</creator><creator>Noguer, Thierry</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SU</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>KR7</scope><scope>7QL</scope><scope>7QO</scope><scope>7ST</scope><scope>7T7</scope><scope>P64</scope><scope>SOI</scope><scope>7X8</scope></search><sort><creationdate>2010</creationdate><title>Biosensor-controlled degradation of chlorpyrifos and chlorfenvinfos using a phosphotriesterase-based detoxification column</title><author>Istamboulie, Georges ; Durbiano, Romain ; Fournier, Didier ; Marty, Jean-Louis ; Noguer, Thierry</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c471t-592f44406b4a4a3d06e23c261d1df566f163f6fb08ffd5faf84036be918cf7723</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Acetylcholinesterase</topic><topic>Bacteria</topic><topic>Biosensing Techniques - methods</topic><topic>Biosensor</topic><topic>Chlorfenvinphos - analysis</topic><topic>Chlorfenvinphos - metabolism</topic><topic>Chlorpyrifos</topic><topic>Chlorpyrifos - analysis</topic><topic>Chlorpyrifos - metabolism</topic><topic>Covalence</topic><topic>Decontamination</topic><topic>Degradation</topic><topic>Enzymes, Immobilized - metabolism</topic><topic>Insecticides</topic><topic>Insecticides - analysis</topic><topic>Insecticides - metabolism</topic><topic>Organophosphates</topic><topic>Pesticides</topic><topic>Phosphoric Triester Hydrolases - metabolism</topic><topic>Phosphotriesterase</topic><topic>Water Pollutants, Chemical - analysis</topic><topic>Water Pollutants, Chemical - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Istamboulie, Georges</creatorcontrib><creatorcontrib>Durbiano, Romain</creatorcontrib><creatorcontrib>Fournier, Didier</creatorcontrib><creatorcontrib>Marty, Jean-Louis</creatorcontrib><creatorcontrib>Noguer, Thierry</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Environmental Engineering Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Civil Engineering Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Chemosphere (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Istamboulie, Georges</au><au>Durbiano, Romain</au><au>Fournier, Didier</au><au>Marty, Jean-Louis</au><au>Noguer, Thierry</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biosensor-controlled degradation of chlorpyrifos and chlorfenvinfos using a phosphotriesterase-based detoxification column</atitle><jtitle>Chemosphere (Oxford)</jtitle><addtitle>Chemosphere</addtitle><date>2010</date><risdate>2010</risdate><volume>78</volume><issue>1</issue><spage>1</spage><epage>6</epage><pages>1-6</pages><issn>0045-6535</issn><eissn>1879-1298</eissn><abstract>This works presents the development of a detoxification system based on bacterial phosphotriesterase (PTE) for the degradation of organophosphate (OP) insecticides in water. PTE was immobilised on an activated agarose gel via covalent coupling. Two different OPs were studied, chlorpyrifos and chlorfenvinfos, due to their importance in the field of water policy. The efficiency of insecticide degradation was controlled using a highly sensitive biosensor allowing the detection of OP concentration as low as 0.004
μg
L
−1. Under optimum conditions, it was shown that a column incorporating 500
IU of PTE was suitable for the detoxification of solutions containing either isolated pesticides or pesticides mixtures, even at concentrations higher than authorized limits. Finally, the method was shown to be adapted to the decontamination of real samples of pesticides with concentrations up to 20
μg
L
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| subjects | Acetylcholinesterase Bacteria Biosensing Techniques - methods Biosensor Chlorfenvinphos - analysis Chlorfenvinphos - metabolism Chlorpyrifos Chlorpyrifos - analysis Chlorpyrifos - metabolism Covalence Decontamination Degradation Enzymes, Immobilized - metabolism Insecticides Insecticides - analysis Insecticides - metabolism Organophosphates Pesticides Phosphoric Triester Hydrolases - metabolism Phosphotriesterase Water Pollutants, Chemical - analysis Water Pollutants, Chemical - metabolism |
| title | Biosensor-controlled degradation of chlorpyrifos and chlorfenvinfos using a phosphotriesterase-based detoxification column |
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