Effect of aspirin on the fibrinolytic response in perfused rat hindquarters
The role of aspirin in tissue plasminogen activator (t-PA) release was studied in rats after experimental venous occlusion. For this purpose, we developed a new experimental model which combines a vascular perfusion system (isolated rat hindquarters) with vascular stimulation, namely the application...
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| Veröffentlicht in: | European journal of pharmacology 1992-12, Vol.229 (1), p.39-44 |
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| container_title | European journal of pharmacology |
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| creator | Iacoviello, Licia De Curtis, Amalia Amore, Concetta D'Adamo, Maria Cristina Buczko, Wlodzimierz de Gaetano, Giovanni Donati, Maria Benedetta |
| description | The role of aspirin in tissue plasminogen activator (t-PA) release was studied in rats after experimental venous occlusion. For this purpose, we developed a new experimental model which combines a vascular perfusion system (isolated rat hindquarters) with vascular stimulation, namely the application of venous stasis. Application of venous stasis for 30 min induced the release of t-PA from the vascular endothelium into the perfusate (from 0.19 ± 0.05 to 0.39 ± 0.05 UI/ml), reaching a peak 90 s after reperfusion. Aspirin administered to rats 60 min before the experiments (100 mg/kg i.v.), or dissolved in Tyrode solution (100 μM), suppressed 6-keto-prostaglandin F
1α (6-keto-PGF
1α) synthesis (0.38 ± 0.09 in control and |
| doi_str_mv | 10.1016/0014-2999(92)90283-A |
| format | Article |
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1α (6-keto-PGF
1α) synthesis (0.38 ± 0.09 in control and <0.01 and 0.15 ± 0.09 ng/ml, respectively, in aspirin-treated groups) but did not prevent the increase in fibrinolytic activity after venous occlusion (from 0.20 ± 0.04 to 0.38 ± 0.06 and from 0.07 ± 0.03 to 0.27 ± 0.03 IU/ml, respectively, in the aspirin-treated group). Our results suggest that the increase in fibrinolytic activity after experimental venous occlusion in isolated rat hindlegs is modulated by mechanism(s) other than the cyclooxygenase pathway in the vascular wall.</description><identifier>ISSN: 0014-2999</identifier><identifier>EISSN: 1879-0712</identifier><identifier>DOI: 10.1016/0014-2999(92)90283-A</identifier><identifier>PMID: 1473562</identifier><identifier>CODEN: EJPHAZ</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>6-Ketoprostaglandin F1 alpha - metabolism ; Animals ; Aspirin ; Aspirin - pharmacology ; Biological and medical sciences ; Blood. Blood coagulation. Reticuloendothelial system ; Disease Models, Animal ; Endothelium, Vascular - drug effects ; Endothelium, Vascular - metabolism ; Fibrinolysis - drug effects ; Fibrinolytic activity ; Hindlimb - blood supply ; Ligation ; Male ; Medical sciences ; Perfusion ; Pharmacology. Drug treatments ; Rats ; Tissue Plasminogen Activator - metabolism ; Veins ; Venous Insufficiency - blood ; Venous stasis</subject><ispartof>European journal of pharmacology, 1992-12, Vol.229 (1), p.39-44</ispartof><rights>1992</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c386t-a8b49649efc39e4dfd49ba4a4af420dc85cf83108a3adec93bcb9e0508fd68b23</citedby><cites>FETCH-LOGICAL-c386t-a8b49649efc39e4dfd49ba4a4af420dc85cf83108a3adec93bcb9e0508fd68b23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0014-2999(92)90283-A$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4475556$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1473562$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Iacoviello, Licia</creatorcontrib><creatorcontrib>De Curtis, Amalia</creatorcontrib><creatorcontrib>Amore, Concetta</creatorcontrib><creatorcontrib>D'Adamo, Maria Cristina</creatorcontrib><creatorcontrib>Buczko, Wlodzimierz</creatorcontrib><creatorcontrib>de Gaetano, Giovanni</creatorcontrib><creatorcontrib>Donati, Maria Benedetta</creatorcontrib><title>Effect of aspirin on the fibrinolytic response in perfused rat hindquarters</title><title>European journal of pharmacology</title><addtitle>Eur J Pharmacol</addtitle><description>The role of aspirin in tissue plasminogen activator (t-PA) release was studied in rats after experimental venous occlusion. For this purpose, we developed a new experimental model which combines a vascular perfusion system (isolated rat hindquarters) with vascular stimulation, namely the application of venous stasis. Application of venous stasis for 30 min induced the release of t-PA from the vascular endothelium into the perfusate (from 0.19 ± 0.05 to 0.39 ± 0.05 UI/ml), reaching a peak 90 s after reperfusion. Aspirin administered to rats 60 min before the experiments (100 mg/kg i.v.), or dissolved in Tyrode solution (100 μM), suppressed 6-keto-prostaglandin F
1α (6-keto-PGF
1α) synthesis (0.38 ± 0.09 in control and <0.01 and 0.15 ± 0.09 ng/ml, respectively, in aspirin-treated groups) but did not prevent the increase in fibrinolytic activity after venous occlusion (from 0.20 ± 0.04 to 0.38 ± 0.06 and from 0.07 ± 0.03 to 0.27 ± 0.03 IU/ml, respectively, in the aspirin-treated group). Our results suggest that the increase in fibrinolytic activity after experimental venous occlusion in isolated rat hindlegs is modulated by mechanism(s) other than the cyclooxygenase pathway in the vascular wall.</description><subject>6-Ketoprostaglandin F1 alpha - metabolism</subject><subject>Animals</subject><subject>Aspirin</subject><subject>Aspirin - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Blood. Blood coagulation. Reticuloendothelial system</subject><subject>Disease Models, Animal</subject><subject>Endothelium, Vascular - drug effects</subject><subject>Endothelium, Vascular - metabolism</subject><subject>Fibrinolysis - drug effects</subject><subject>Fibrinolytic activity</subject><subject>Hindlimb - blood supply</subject><subject>Ligation</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Perfusion</subject><subject>Pharmacology. Drug treatments</subject><subject>Rats</subject><subject>Tissue Plasminogen Activator - metabolism</subject><subject>Veins</subject><subject>Venous Insufficiency - blood</subject><subject>Venous stasis</subject><issn>0014-2999</issn><issn>1879-0712</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1LxDAQhoMouq7-A4UcRPRQzVfb5CIs4hcKXvQc0mSCkW5bk1bw35t1F71JBsLwPjMMD0JHlFxQQqtLQqgomFLqTLFzRZjkxWILzaisVUFqyrbR7BfZQ_spvRNCSsXKXbRLRc3Lis3Q4433YEfce2zSEGLocN_h8Q2wD03u-vZrDBZHSEPfJcA5HyD6KYHD0Yz4LXTuYzJxhJgO0I43bYLDzT9Hr7c3L9f3xdPz3cP14qmwXFZjYWQjVCUUeMsVCOedUI0R-XnBiLOytF5ySqThxoFVvLGNAlIS6V0lG8bn6HS9d4j9xwRp1MuQLLSt6aCfkq65oILnmiOxBm3sU4rg9RDD0sQvTYleOdQrQXolSCumfxzqRR473uyfmiW4v6G1tJyfbHKTrGl9NJ0N6RcToi7LssrY1RqD7OIzQNTJBugsuBCzcu368P8d370OjoY</recordid><startdate>19921208</startdate><enddate>19921208</enddate><creator>Iacoviello, Licia</creator><creator>De Curtis, Amalia</creator><creator>Amore, Concetta</creator><creator>D'Adamo, Maria Cristina</creator><creator>Buczko, Wlodzimierz</creator><creator>de Gaetano, Giovanni</creator><creator>Donati, Maria Benedetta</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19921208</creationdate><title>Effect of aspirin on the fibrinolytic response in perfused rat hindquarters</title><author>Iacoviello, Licia ; De Curtis, Amalia ; Amore, Concetta ; D'Adamo, Maria Cristina ; Buczko, Wlodzimierz ; de Gaetano, Giovanni ; Donati, Maria Benedetta</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c386t-a8b49649efc39e4dfd49ba4a4af420dc85cf83108a3adec93bcb9e0508fd68b23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>6-Ketoprostaglandin F1 alpha - metabolism</topic><topic>Animals</topic><topic>Aspirin</topic><topic>Aspirin - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Blood. Blood coagulation. Reticuloendothelial system</topic><topic>Disease Models, Animal</topic><topic>Endothelium, Vascular - drug effects</topic><topic>Endothelium, Vascular - metabolism</topic><topic>Fibrinolysis - drug effects</topic><topic>Fibrinolytic activity</topic><topic>Hindlimb - blood supply</topic><topic>Ligation</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Perfusion</topic><topic>Pharmacology. Drug treatments</topic><topic>Rats</topic><topic>Tissue Plasminogen Activator - metabolism</topic><topic>Veins</topic><topic>Venous Insufficiency - blood</topic><topic>Venous stasis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Iacoviello, Licia</creatorcontrib><creatorcontrib>De Curtis, Amalia</creatorcontrib><creatorcontrib>Amore, Concetta</creatorcontrib><creatorcontrib>D'Adamo, Maria Cristina</creatorcontrib><creatorcontrib>Buczko, Wlodzimierz</creatorcontrib><creatorcontrib>de Gaetano, Giovanni</creatorcontrib><creatorcontrib>Donati, Maria Benedetta</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>European journal of pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Iacoviello, Licia</au><au>De Curtis, Amalia</au><au>Amore, Concetta</au><au>D'Adamo, Maria Cristina</au><au>Buczko, Wlodzimierz</au><au>de Gaetano, Giovanni</au><au>Donati, Maria Benedetta</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of aspirin on the fibrinolytic response in perfused rat hindquarters</atitle><jtitle>European journal of pharmacology</jtitle><addtitle>Eur J Pharmacol</addtitle><date>1992-12-08</date><risdate>1992</risdate><volume>229</volume><issue>1</issue><spage>39</spage><epage>44</epage><pages>39-44</pages><issn>0014-2999</issn><eissn>1879-0712</eissn><coden>EJPHAZ</coden><abstract>The role of aspirin in tissue plasminogen activator (t-PA) release was studied in rats after experimental venous occlusion. For this purpose, we developed a new experimental model which combines a vascular perfusion system (isolated rat hindquarters) with vascular stimulation, namely the application of venous stasis. Application of venous stasis for 30 min induced the release of t-PA from the vascular endothelium into the perfusate (from 0.19 ± 0.05 to 0.39 ± 0.05 UI/ml), reaching a peak 90 s after reperfusion. Aspirin administered to rats 60 min before the experiments (100 mg/kg i.v.), or dissolved in Tyrode solution (100 μM), suppressed 6-keto-prostaglandin F
1α (6-keto-PGF
1α) synthesis (0.38 ± 0.09 in control and <0.01 and 0.15 ± 0.09 ng/ml, respectively, in aspirin-treated groups) but did not prevent the increase in fibrinolytic activity after venous occlusion (from 0.20 ± 0.04 to 0.38 ± 0.06 and from 0.07 ± 0.03 to 0.27 ± 0.03 IU/ml, respectively, in the aspirin-treated group). Our results suggest that the increase in fibrinolytic activity after experimental venous occlusion in isolated rat hindlegs is modulated by mechanism(s) other than the cyclooxygenase pathway in the vascular wall.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>1473562</pmid><doi>10.1016/0014-2999(92)90283-A</doi><tpages>6</tpages></addata></record> |
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| subjects | 6-Ketoprostaglandin F1 alpha - metabolism Animals Aspirin Aspirin - pharmacology Biological and medical sciences Blood. Blood coagulation. Reticuloendothelial system Disease Models, Animal Endothelium, Vascular - drug effects Endothelium, Vascular - metabolism Fibrinolysis - drug effects Fibrinolytic activity Hindlimb - blood supply Ligation Male Medical sciences Perfusion Pharmacology. Drug treatments Rats Tissue Plasminogen Activator - metabolism Veins Venous Insufficiency - blood Venous stasis |
| title | Effect of aspirin on the fibrinolytic response in perfused rat hindquarters |
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