Potential of the Sebia Capillarys® neonat fast automated system for neonatal screening of sickle cell disease

Background: Most screening programs for sickle cell disease (SCD) utilize isoelectric focusing (IEF) or high performance liquid chromatography (HPLC) to detect haemoglobin (Hb) variants. The first method is not automated and becomes too tedious when many samples have to be investigated. The aim of t...

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Veröffentlicht in:Clinical chemistry and laboratory medicine 2009, Vol.47 (11), p.1423-1432
Hauptverfasser: Renom, Gilles, Mereau, Claude, Maboudou, Patrice, Périni, Jean-Marc
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container_issue 11
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container_title Clinical chemistry and laboratory medicine
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creator Renom, Gilles
Mereau, Claude
Maboudou, Patrice
Périni, Jean-Marc
description Background: Most screening programs for sickle cell disease (SCD) utilize isoelectric focusing (IEF) or high performance liquid chromatography (HPLC) to detect haemoglobin (Hb) variants. The first method is not automated and becomes too tedious when many samples have to be investigated. The aim of this work is to explore the capacity of an automated capillary electrophoresis (CE) system, with full traceability, as a tool for newborn screening of SCD. Methods: The Capillarys® neonat fast automated system has been developed by Sebia for newborn screening. We performed separate studies using different types of samples to evaluate the utility of the Capillarys® for (i) separating Hb S and other variants, and (ii) for performing the routine activity of our laboratory for 20 working days. Results: A throughput of 48 samples per hour with a loading capacity of 192 samples was achieved. Migration times of the major Hb variants were distinct. There were few variants showing similar migration times to Hb S and Hb C and thalassaemia could be detected. In addition, late screening, screening of premature or transfused babies and screening performed using poor quality Guthrie's cards did not interfere with reporting of accurate phenotypes. Conclusions: Sebia Capillarys® neonat fast automated system is a reliable tool for haemoglobinopathy neonatal screening. Clin Chem Lab Med 2009;47:1423–32.
doi_str_mv 10.1515/CCLM.2009.315
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In addition, late screening, screening of premature or transfused babies and screening performed using poor quality Guthrie's cards did not interfere with reporting of accurate phenotypes. Conclusions: Sebia Capillarys® neonat fast automated system is a reliable tool for haemoglobinopathy neonatal screening. Clin Chem Lab Med 2009;47:1423–32.</abstract><cop>Berlin</cop><pub>Walter de Gruyter</pub><pmid>19912048</pmid><doi>10.1515/CCLM.2009.315</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record>
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subjects Anemia, Sickle Cell - diagnosis
Anemia, Sickle Cell - genetics
Anemias. Hemoglobinopathies
Autoanalysis
Biological and medical sciences
capillary electrophoresis
Cohort Studies
Diseases of red blood cells
Electrophoresis, Capillary
General aspects
Hematologic and hematopoietic diseases
Humans
Infant, Newborn
Investigative techniques, diagnostic techniques (general aspects)
Medical sciences
Neonatal Screening - instrumentation
Neonatal Screening - methods
newborn screening
Phenotype
Reproducibility of Results
Sensitivity and Specificity
sickle cell disease
thalassaemia
title Potential of the Sebia Capillarys® neonat fast automated system for neonatal screening of sickle cell disease
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