Salmonella Infection of Gallbladder Epithelial Cells Drives Local Inflammation and Injury in a Model of Acute Typhoid Fever
The gallbladder is often colonized by Salmonella during typhoid fever, yet little is known about bacterial pathogenesis in this organ. With use of a mouse model of acute typhoid fever, we demonstrate that Salmonella infect gallbladder epithelial cells in vivo. Bacteria in the gallbladder showed a un...
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| Veröffentlicht in: | The Journal of infectious diseases 2009-12, Vol.200 (11), p.1703-1713 |
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| container_title | The Journal of infectious diseases |
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| creator | Menendez, Alfredo Arena, Ellen T. Guttman, Julian A. Thorson, Lisa Vallance, Bruce A. Vogl, Wayne Finlay, B. Brett |
| description | The gallbladder is often colonized by Salmonella during typhoid fever, yet little is known about bacterial pathogenesis in this organ. With use of a mouse model of acute typhoid fever, we demonstrate that Salmonella infect gallbladder epithelial cells in vivo. Bacteria in the gallbladder showed a unique behavior as they replicated within gallbladder epithelial cells and remained confined to those cells without translocating to the mucosa. Infected gallbladders showed histopathological damage characterized by destruction of the epithelium and massive infiltration of neutrophils, accompanied by a local increase of proinflammatory cytokines. Damage was determined by the ability of Salmonella to invade gallbladder epithelial cells and was independent of high numbers of replication-competent, although invasion-deficient, bacteria in the lumen. Our results establish gallbladder epithelial cells as a novel niche for in vivo replication of Salmonella and reveal the involvement of these cells in the pathogenesis of Salmonella in the gallbladder during the course of acute typhoid fever |
| doi_str_mv | 10.1086/646608 |
| format | Article |
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Brett</creator><creatorcontrib>Menendez, Alfredo ; Arena, Ellen T. ; Guttman, Julian A. ; Thorson, Lisa ; Vallance, Bruce A. ; Vogl, Wayne ; Finlay, B. Brett</creatorcontrib><description>The gallbladder is often colonized by Salmonella during typhoid fever, yet little is known about bacterial pathogenesis in this organ. With use of a mouse model of acute typhoid fever, we demonstrate that Salmonella infect gallbladder epithelial cells in vivo. Bacteria in the gallbladder showed a unique behavior as they replicated within gallbladder epithelial cells and remained confined to those cells without translocating to the mucosa. Infected gallbladders showed histopathological damage characterized by destruction of the epithelium and massive infiltration of neutrophils, accompanied by a local increase of proinflammatory cytokines. Damage was determined by the ability of Salmonella to invade gallbladder epithelial cells and was independent of high numbers of replication-competent, although invasion-deficient, bacteria in the lumen. Our results establish gallbladder epithelial cells as a novel niche for in vivo replication of Salmonella and reveal the involvement of these cells in the pathogenesis of Salmonella in the gallbladder during the course of acute typhoid fever</description><identifier>ISSN: 0022-1899</identifier><identifier>EISSN: 1537-6613</identifier><identifier>DOI: 10.1086/646608</identifier><identifier>PMID: 19852670</identifier><identifier>CODEN: JIDIAQ</identifier><language>eng</language><publisher>Oxford: The University of Chicago Press</publisher><subject>Animals ; Bacteria ; Bacterial diseases ; Bacterial diseases of the digestive system and abdomen ; Bile ; Biological and medical sciences ; Cholecystitis, Acute - microbiology ; Cholecystitis, Acute - pathology ; Colony Count, Microbial ; Cytokines ; Cytokines - metabolism ; Data Interpretation, Statistical ; Disease Models, Animal ; Epithelial cells ; Epithelial Cells - microbiology ; Epithelial Cells - pathology ; Female ; Fundamental and applied biological sciences. Psychology ; Gallbladder ; Gallbladder - metabolism ; Gallbladder - microbiology ; Gallbladder - pathology ; Histocytochemistry ; Human bacterial diseases ; Infections ; Infectious diseases ; Inflammation - microbiology ; Inflammation - pathology ; Liver ; Liver - metabolism ; Medical sciences ; Mice ; Mice, Inbred C57BL ; Microbiology ; Neutrophil Infiltration ; Salmonella ; Salmonella Infections, Animal - microbiology ; Salmonella Infections, Animal - pathology ; Salmonella typhi - growth & development ; Salmonella typhimurium ; Typhoid fever ; Typhoid Fever - microbiology ; Typhoid Fever - pathology</subject><ispartof>The Journal of infectious diseases, 2009-12, Vol.200 (11), p.1703-1713</ispartof><rights>2009 Infectious Diseases Society of America</rights><rights>2009 by the Infectious Diseases Society of America 2009</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c425t-a1b150bbf6ce287864f92c5c80e9e28bd262668e812e7eb4e94bc72d5cda3d943</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/27794332$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/27794332$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,780,784,803,27924,27925,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22159239$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19852670$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Menendez, Alfredo</creatorcontrib><creatorcontrib>Arena, Ellen T.</creatorcontrib><creatorcontrib>Guttman, Julian A.</creatorcontrib><creatorcontrib>Thorson, Lisa</creatorcontrib><creatorcontrib>Vallance, Bruce A.</creatorcontrib><creatorcontrib>Vogl, Wayne</creatorcontrib><creatorcontrib>Finlay, B. Brett</creatorcontrib><title>Salmonella Infection of Gallbladder Epithelial Cells Drives Local Inflammation and Injury in a Model of Acute Typhoid Fever</title><title>The Journal of infectious diseases</title><addtitle>The Journal of Infectious Diseases</addtitle><addtitle>The Journal of Infectious Diseases</addtitle><description>The gallbladder is often colonized by Salmonella during typhoid fever, yet little is known about bacterial pathogenesis in this organ. With use of a mouse model of acute typhoid fever, we demonstrate that Salmonella infect gallbladder epithelial cells in vivo. Bacteria in the gallbladder showed a unique behavior as they replicated within gallbladder epithelial cells and remained confined to those cells without translocating to the mucosa. Infected gallbladders showed histopathological damage characterized by destruction of the epithelium and massive infiltration of neutrophils, accompanied by a local increase of proinflammatory cytokines. Damage was determined by the ability of Salmonella to invade gallbladder epithelial cells and was independent of high numbers of replication-competent, although invasion-deficient, bacteria in the lumen. Our results establish gallbladder epithelial cells as a novel niche for in vivo replication of Salmonella and reveal the involvement of these cells in the pathogenesis of Salmonella in the gallbladder during the course of acute typhoid fever</description><subject>Animals</subject><subject>Bacteria</subject><subject>Bacterial diseases</subject><subject>Bacterial diseases of the digestive system and abdomen</subject><subject>Bile</subject><subject>Biological and medical sciences</subject><subject>Cholecystitis, Acute - microbiology</subject><subject>Cholecystitis, Acute - pathology</subject><subject>Colony Count, Microbial</subject><subject>Cytokines</subject><subject>Cytokines - metabolism</subject><subject>Data Interpretation, Statistical</subject><subject>Disease Models, Animal</subject><subject>Epithelial cells</subject><subject>Epithelial Cells - microbiology</subject><subject>Epithelial Cells - pathology</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gallbladder</subject><subject>Gallbladder - metabolism</subject><subject>Gallbladder - microbiology</subject><subject>Gallbladder - pathology</subject><subject>Histocytochemistry</subject><subject>Human bacterial diseases</subject><subject>Infections</subject><subject>Infectious diseases</subject><subject>Inflammation - microbiology</subject><subject>Inflammation - pathology</subject><subject>Liver</subject><subject>Liver - metabolism</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Microbiology</subject><subject>Neutrophil Infiltration</subject><subject>Salmonella</subject><subject>Salmonella Infections, Animal - microbiology</subject><subject>Salmonella Infections, Animal - pathology</subject><subject>Salmonella typhi - growth & development</subject><subject>Salmonella typhimurium</subject><subject>Typhoid fever</subject><subject>Typhoid Fever - microbiology</subject><subject>Typhoid Fever - pathology</subject><issn>0022-1899</issn><issn>1537-6613</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kUFv1DAQhSMEokuBfwAyB-AUsB3HTo7V0m6LgjiwSGgvlmNP1CxOnNpJxYo_j0NW3ROcLL_55o3mTZK8JPgDwQX_yBnnuHiUrEieiZRzkj1OVhhTmpKiLM-SZyHsMcYs4-JpckbKIqdc4FXy-5uynevBWoVu-gb02LoeuQZtlLW1VcaAR5dDO96CbZVF60gG9Mm39xBQ5XSUYptVXaf-dqreRGE_-QNq4w99cQbs7HehpxHQ9jDcutagK7gH_zx50igb4MXxPU--X11u19dp9XVzs76oUs1oPqaK1CTHdd1wDbQQBWdNSXWuCwxlFGpDOeW8gIJQEFAzKFmtBTW5NiozJcvOk_eL7-Dd3QRhlF0b9LxyD24KUmSMxFzymXz3X5ISyhlj5QnU3oXgoZGDbzvlD5JgOR9ELgeJ4Ouj41R3YE7Y8QIReHsEVIhpNl71ug0PHKUkL2k2T3yzcG4a_j3s1cLsw-j8yUOImEJGYz1d6m0Y4ddDXfmfkotM5PL6x05WeLv7vKm2cpf9ATeMtR0</recordid><startdate>20091201</startdate><enddate>20091201</enddate><creator>Menendez, Alfredo</creator><creator>Arena, Ellen T.</creator><creator>Guttman, Julian A.</creator><creator>Thorson, Lisa</creator><creator>Vallance, Bruce A.</creator><creator>Vogl, Wayne</creator><creator>Finlay, B. Brett</creator><general>The University of Chicago Press</general><general>University of Chicago Press</general><general>Oxford University Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20091201</creationdate><title>Salmonella Infection of Gallbladder Epithelial Cells Drives Local Inflammation and Injury in a Model of Acute Typhoid Fever</title><author>Menendez, Alfredo ; Arena, Ellen T. ; Guttman, Julian A. ; Thorson, Lisa ; Vallance, Bruce A. ; Vogl, Wayne ; Finlay, B. Brett</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c425t-a1b150bbf6ce287864f92c5c80e9e28bd262668e812e7eb4e94bc72d5cda3d943</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Animals</topic><topic>Bacteria</topic><topic>Bacterial diseases</topic><topic>Bacterial diseases of the digestive system and abdomen</topic><topic>Bile</topic><topic>Biological and medical sciences</topic><topic>Cholecystitis, Acute - microbiology</topic><topic>Cholecystitis, Acute - pathology</topic><topic>Colony Count, Microbial</topic><topic>Cytokines</topic><topic>Cytokines - metabolism</topic><topic>Data Interpretation, Statistical</topic><topic>Disease Models, Animal</topic><topic>Epithelial cells</topic><topic>Epithelial Cells - microbiology</topic><topic>Epithelial Cells - pathology</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gallbladder</topic><topic>Gallbladder - metabolism</topic><topic>Gallbladder - microbiology</topic><topic>Gallbladder - pathology</topic><topic>Histocytochemistry</topic><topic>Human bacterial diseases</topic><topic>Infections</topic><topic>Infectious diseases</topic><topic>Inflammation - microbiology</topic><topic>Inflammation - pathology</topic><topic>Liver</topic><topic>Liver - metabolism</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Microbiology</topic><topic>Neutrophil Infiltration</topic><topic>Salmonella</topic><topic>Salmonella Infections, Animal - microbiology</topic><topic>Salmonella Infections, Animal - pathology</topic><topic>Salmonella typhi - growth & development</topic><topic>Salmonella typhimurium</topic><topic>Typhoid fever</topic><topic>Typhoid Fever - microbiology</topic><topic>Typhoid Fever - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Menendez, Alfredo</creatorcontrib><creatorcontrib>Arena, Ellen T.</creatorcontrib><creatorcontrib>Guttman, Julian A.</creatorcontrib><creatorcontrib>Thorson, Lisa</creatorcontrib><creatorcontrib>Vallance, Bruce A.</creatorcontrib><creatorcontrib>Vogl, Wayne</creatorcontrib><creatorcontrib>Finlay, B. 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Brett</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Salmonella Infection of Gallbladder Epithelial Cells Drives Local Inflammation and Injury in a Model of Acute Typhoid Fever</atitle><jtitle>The Journal of infectious diseases</jtitle><stitle>The Journal of Infectious Diseases</stitle><addtitle>The Journal of Infectious Diseases</addtitle><date>2009-12-01</date><risdate>2009</risdate><volume>200</volume><issue>11</issue><spage>1703</spage><epage>1713</epage><pages>1703-1713</pages><issn>0022-1899</issn><eissn>1537-6613</eissn><coden>JIDIAQ</coden><abstract>The gallbladder is often colonized by Salmonella during typhoid fever, yet little is known about bacterial pathogenesis in this organ. With use of a mouse model of acute typhoid fever, we demonstrate that Salmonella infect gallbladder epithelial cells in vivo. Bacteria in the gallbladder showed a unique behavior as they replicated within gallbladder epithelial cells and remained confined to those cells without translocating to the mucosa. Infected gallbladders showed histopathological damage characterized by destruction of the epithelium and massive infiltration of neutrophils, accompanied by a local increase of proinflammatory cytokines. Damage was determined by the ability of Salmonella to invade gallbladder epithelial cells and was independent of high numbers of replication-competent, although invasion-deficient, bacteria in the lumen. Our results establish gallbladder epithelial cells as a novel niche for in vivo replication of Salmonella and reveal the involvement of these cells in the pathogenesis of Salmonella in the gallbladder during the course of acute typhoid fever</abstract><cop>Oxford</cop><pub>The University of Chicago Press</pub><pmid>19852670</pmid><doi>10.1086/646608</doi><tpages>11</tpages></addata></record> |
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| ispartof | The Journal of infectious diseases, 2009-12, Vol.200 (11), p.1703-1713 |
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| subjects | Animals Bacteria Bacterial diseases Bacterial diseases of the digestive system and abdomen Bile Biological and medical sciences Cholecystitis, Acute - microbiology Cholecystitis, Acute - pathology Colony Count, Microbial Cytokines Cytokines - metabolism Data Interpretation, Statistical Disease Models, Animal Epithelial cells Epithelial Cells - microbiology Epithelial Cells - pathology Female Fundamental and applied biological sciences. Psychology Gallbladder Gallbladder - metabolism Gallbladder - microbiology Gallbladder - pathology Histocytochemistry Human bacterial diseases Infections Infectious diseases Inflammation - microbiology Inflammation - pathology Liver Liver - metabolism Medical sciences Mice Mice, Inbred C57BL Microbiology Neutrophil Infiltration Salmonella Salmonella Infections, Animal - microbiology Salmonella Infections, Animal - pathology Salmonella typhi - growth & development Salmonella typhimurium Typhoid fever Typhoid Fever - microbiology Typhoid Fever - pathology |
| title | Salmonella Infection of Gallbladder Epithelial Cells Drives Local Inflammation and Injury in a Model of Acute Typhoid Fever |
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