A Grass Molecular Identification System for Forensic Botany: A Critical Evaluation of the Strengths and Limitations
: Plant material is frequently encountered in criminal investigations but often overlooked as potential evidence. We designed a DNA‐based molecular identification system for 100 Australian grasses that consisted of a series of polymerase chain reaction assays that enabled the progressive identifica...
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| Veröffentlicht in: | Journal of forensic sciences 2009-11, Vol.54 (6), p.1254-1260 |
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| container_title | Journal of forensic sciences |
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| creator | Ward, Jodie Gilmore, Simon R. Robertson, James Peakall, Rod |
| description | : Plant material is frequently encountered in criminal investigations but often overlooked as potential evidence. We designed a DNA‐based molecular identification system for 100 Australian grasses that consisted of a series of polymerase chain reaction assays that enabled the progressive identification of grasses to different taxonomic levels. The identification system was based on DNA sequence variation at four chloroplast and two mitochondrial loci. Seventeen informative indels and 68 single‐nucleotide polymorphisms were utilized as molecular markers for subfamily to species‐level identification. To identify an unknown sample to subfamily level required a minimum of four markers or nine markers for species identification. The accuracy of the system was confirmed by blind tests. We have demonstrated “proof of concept” of a molecular identification system for trace botanical samples. Our evaluation suggests that the adoption of a system that combines this approach with DNA sequencing could assist the morphological identification of grasses found as forensic evidence. |
| doi_str_mv | 10.1111/j.1556-4029.2009.01196.x |
| format | Article |
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We designed a DNA‐based molecular identification system for 100 Australian grasses that consisted of a series of polymerase chain reaction assays that enabled the progressive identification of grasses to different taxonomic levels. The identification system was based on DNA sequence variation at four chloroplast and two mitochondrial loci. Seventeen informative indels and 68 single‐nucleotide polymorphisms were utilized as molecular markers for subfamily to species‐level identification. To identify an unknown sample to subfamily level required a minimum of four markers or nine markers for species identification. The accuracy of the system was confirmed by blind tests. We have demonstrated “proof of concept” of a molecular identification system for trace botanical samples. 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We designed a DNA‐based molecular identification system for 100 Australian grasses that consisted of a series of polymerase chain reaction assays that enabled the progressive identification of grasses to different taxonomic levels. The identification system was based on DNA sequence variation at four chloroplast and two mitochondrial loci. Seventeen informative indels and 68 single‐nucleotide polymorphisms were utilized as molecular markers for subfamily to species‐level identification. To identify an unknown sample to subfamily level required a minimum of four markers or nine markers for species identification. The accuracy of the system was confirmed by blind tests. We have demonstrated “proof of concept” of a molecular identification system for trace botanical samples. Our evaluation suggests that the adoption of a system that combines this approach with DNA sequencing could assist the morphological identification of grasses found as forensic evidence.</description><subject>Botany</subject><subject>chloroplast DNA</subject><subject>Chloroplasts - metabolism</subject><subject>Criminal investigations</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA Primers</subject><subject>DNA, Mitochondrial - genetics</subject><subject>DNA, Plant - genetics</subject><subject>Evidence</subject><subject>forensic botany</subject><subject>Forensic Medicine</subject><subject>forensic science</subject><subject>Forensic sciences</subject><subject>Genetic Markers</subject><subject>Grasses</subject><subject>Humans</subject><subject>indels</subject><subject>mitochondrial DNA</subject><subject>molecular identification system</subject><subject>Poaceae - genetics</subject><subject>Polymerase Chain Reaction</subject><subject>Polymorphism</subject><subject>Polymorphism, Single Nucleotide</subject><subject>Sequence Analysis, DNA</subject><subject>single nucleotide polymorphisms</subject><subject>species identification</subject><subject>Species Specificity</subject><issn>0022-1198</issn><issn>1556-4029</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU9v0zAYxi0EYmXwFZDFhVOC_8WJkTiUspZBtx02qMTFchObuSTxsB1ovz3OUg2JE77Y8vt7Hr16HgAgRjlO580ux0XBM4aIyAlCIkcYC57vH4HZw-AxmCFESJYm1Ql4FsIOIcQxx0_BSfrCFUZiBsIcrrwKAV64VtdDqzw8b3QfrbG1itb18PoQou6gcR4undd9sDV876LqD2_hHC68jYls4dkv1Q6TwhkYbzW8jon-Hm8DVH0D17az8X4enoMnRrVBvzjep-DL8uxm8TFbX63OF_N1VrOK8qwpiaYN04WoUG0K2hhTcKYIIkgVRDW1UJUgXBPdCFpsCeGMJoIyJliJzZaegteT7513PwcdouxsqHXbql67IciSshROWfJEvvqH3LnB92k5SVKwglA8QtUE1d6F4LWRd952yh8kRnKsRe7kmL4c05djLfK-FrlP0pdH_2Hb6eav8NhDAt5NwG_b6sN_G8tPy6vxlfTZpLeprP2DXvkfkpe0LOTmciUvPmw2N98-f5WX9A-xPKp_</recordid><startdate>200911</startdate><enddate>200911</enddate><creator>Ward, Jodie</creator><creator>Gilmore, Simon R.</creator><creator>Robertson, James</creator><creator>Peakall, Rod</creator><general>Blackwell Publishing Ltd</general><general>Wiley Subscription Services, Inc</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K7.</scope><scope>7X8</scope></search><sort><creationdate>200911</creationdate><title>A Grass Molecular Identification System for Forensic Botany: A Critical Evaluation of the Strengths and Limitations</title><author>Ward, Jodie ; Gilmore, Simon R. ; Robertson, James ; Peakall, Rod</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4836-d72e3d4e5980cf53dff564a2020a52adc9a8926e2ed935b22643f563449471fb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Botany</topic><topic>chloroplast DNA</topic><topic>Chloroplasts - metabolism</topic><topic>Criminal investigations</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA Primers</topic><topic>DNA, Mitochondrial - genetics</topic><topic>DNA, Plant - genetics</topic><topic>Evidence</topic><topic>forensic botany</topic><topic>Forensic Medicine</topic><topic>forensic science</topic><topic>Forensic sciences</topic><topic>Genetic Markers</topic><topic>Grasses</topic><topic>Humans</topic><topic>indels</topic><topic>mitochondrial DNA</topic><topic>molecular identification system</topic><topic>Poaceae - genetics</topic><topic>Polymerase Chain Reaction</topic><topic>Polymorphism</topic><topic>Polymorphism, Single Nucleotide</topic><topic>Sequence Analysis, DNA</topic><topic>single nucleotide polymorphisms</topic><topic>species identification</topic><topic>Species Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ward, Jodie</creatorcontrib><creatorcontrib>Gilmore, Simon R.</creatorcontrib><creatorcontrib>Robertson, James</creatorcontrib><creatorcontrib>Peakall, Rod</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Criminal Justice (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of forensic sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ward, Jodie</au><au>Gilmore, Simon R.</au><au>Robertson, James</au><au>Peakall, Rod</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Grass Molecular Identification System for Forensic Botany: A Critical Evaluation of the Strengths and Limitations</atitle><jtitle>Journal of forensic sciences</jtitle><addtitle>J Forensic Sci</addtitle><date>2009-11</date><risdate>2009</risdate><volume>54</volume><issue>6</issue><spage>1254</spage><epage>1260</epage><pages>1254-1260</pages><issn>0022-1198</issn><eissn>1556-4029</eissn><coden>JFSCAS</coden><abstract>: Plant material is frequently encountered in criminal investigations but often overlooked as potential evidence. 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| ispartof | Journal of forensic sciences, 2009-11, Vol.54 (6), p.1254-1260 |
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| source | MEDLINE; Access via Wiley Online Library |
| subjects | Botany chloroplast DNA Chloroplasts - metabolism Criminal investigations Deoxyribonucleic acid DNA DNA Primers DNA, Mitochondrial - genetics DNA, Plant - genetics Evidence forensic botany Forensic Medicine forensic science Forensic sciences Genetic Markers Grasses Humans indels mitochondrial DNA molecular identification system Poaceae - genetics Polymerase Chain Reaction Polymorphism Polymorphism, Single Nucleotide Sequence Analysis, DNA single nucleotide polymorphisms species identification Species Specificity |
| title | A Grass Molecular Identification System for Forensic Botany: A Critical Evaluation of the Strengths and Limitations |
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