The use of PEGylated poly [2-(N,N-dimethylamino) ethyl methacrylate] as a mucosal DNA delivery vector and the activation of innate immunity and improvement of HIV-1-specific immune responses
Abstract To minimize the cytotoxicity of poly (2-(dimethylamino) ethyl methacrylate) (PDMAEMA) as a gene delivery vector, we synthesized PEGylated PDMAEMA by atom transfer radical polymerization (ATRP). Here we report its effects on transfection efficiency in vitro delivered with a GFP expression pl...
Gespeichert in:
| Veröffentlicht in: | Biomaterials 2010-01, Vol.31 (1), p.115-123 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 123 |
|---|---|
| container_issue | 1 |
| container_start_page | 115 |
| container_title | Biomaterials |
| container_volume | 31 |
| creator | Qiao, Yong Huang, Yang Qiu, Chao Yue, Xinye Deng, Liandong Wan, Yanmin Xing, Jinfeng Zhang, Congyou Yuan, Songhua Dong, Anjie Xu, Jianqing |
| description | Abstract To minimize the cytotoxicity of poly (2-(dimethylamino) ethyl methacrylate) (PDMAEMA) as a gene delivery vector, we synthesized PEGylated PDMAEMA by atom transfer radical polymerization (ATRP). Here we report its effects on transfection efficiency in vitro delivered with a GFP expression plasmid and immunogenicity in vivo after complexed with a HIV gag gene DNA vaccine. mPEG113 - b -PDMAEMA94 was efficient in condensing DNA and formed polyplexes with an average diameter of about 150 nm. The in vitro transfection experiments demonstrated that PEGylation dramatically decreased the cytotoxicity at the N / P ratios above 30, although the transfection efficiency in vitro was reduced. Interestingly, mice in vivo vaccination study clearly showed that PEGylated PDMAEMA used as DNA delivery vector significantly improved the prime effect of DNA vaccine through intranasal administration. Importantly, PEGylated PDMAEMA was further proved its ability to induce cytokines production by murine macrophages. Overall, mPEG- b -PDMAEMA can be used as an efficient DNA vaccine vector which enhances adaptive immune responses by activating innate immunity. |
| doi_str_mv | 10.1016/j.biomaterials.2009.09.032 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_734117932</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>1_s2_0_S0142961209009624</els_id><sourcerecordid>35063598</sourcerecordid><originalsourceid>FETCH-LOGICAL-c496t-95ea5ace8c3f689fc48419cf6298a1608dd4d3f52c852b0b324a183fa169a1c03</originalsourceid><addsrcrecordid>eNqNkltrFDEUxwdR7Fr9ChJ88ALOmstckj4Ipa1toayC1ReRkM2coVknyZrMDMyX87OZ2V1QfFDhQC7nd_7nkPyz7BnBS4JJ9WazXBtvVQ_BqC4uKcZiOQej97IF4TXPS4HL-9kCk4LmoiL0KHsU4wanMy7ow-yIiJqTusaL7MftHaAhAvIt-nBxOXVJtkFb303oC81frl6v8sZY6O9SxhrnX6HdHs1XSocd_xWpiBSyg_ZRdeh8dYoa6MwIYUIj6N4HpFyD-tRJ6d6MqjfezQ2Nc6kcGWsHZ_ppRxm7DX4EC66fkavrzznJ4xa0aY3eo4ACxK13EeLj7EGb3gCeHNbj7NO7i9uzq_zm_eX12elNrgtR9bkoQZVKA9esrbhodcELInRbUcEVqTBvmqJhbUk1L-karxktFOGsTTmhiMbsOHux103DfR8g9tKaqKHrlAM_RFmzgpBaMJrI538lWYkrVgr-T5ASUhSYkQSe7EEdfIwBWrkNxqowSYLlbAi5kb8bQs6GkHPsxnl66DKsLTS_Sg8OSMD5HoD0fKOBIKM24DQ0JqS_k403_9fn7R8yujPOaNV9gwnixg_BzTVERiqx_Dhbc3YmFkmkogX7CQbL5S0</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>21144031</pqid></control><display><type>article</type><title>The use of PEGylated poly [2-(N,N-dimethylamino) ethyl methacrylate] as a mucosal DNA delivery vector and the activation of innate immunity and improvement of HIV-1-specific immune responses</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Qiao, Yong ; Huang, Yang ; Qiu, Chao ; Yue, Xinye ; Deng, Liandong ; Wan, Yanmin ; Xing, Jinfeng ; Zhang, Congyou ; Yuan, Songhua ; Dong, Anjie ; Xu, Jianqing</creator><creatorcontrib>Qiao, Yong ; Huang, Yang ; Qiu, Chao ; Yue, Xinye ; Deng, Liandong ; Wan, Yanmin ; Xing, Jinfeng ; Zhang, Congyou ; Yuan, Songhua ; Dong, Anjie ; Xu, Jianqing</creatorcontrib><description>Abstract To minimize the cytotoxicity of poly (2-(dimethylamino) ethyl methacrylate) (PDMAEMA) as a gene delivery vector, we synthesized PEGylated PDMAEMA by atom transfer radical polymerization (ATRP). Here we report its effects on transfection efficiency in vitro delivered with a GFP expression plasmid and immunogenicity in vivo after complexed with a HIV gag gene DNA vaccine. mPEG113 - b -PDMAEMA94 was efficient in condensing DNA and formed polyplexes with an average diameter of about 150 nm. The in vitro transfection experiments demonstrated that PEGylation dramatically decreased the cytotoxicity at the N / P ratios above 30, although the transfection efficiency in vitro was reduced. Interestingly, mice in vivo vaccination study clearly showed that PEGylated PDMAEMA used as DNA delivery vector significantly improved the prime effect of DNA vaccine through intranasal administration. Importantly, PEGylated PDMAEMA was further proved its ability to induce cytokines production by murine macrophages. Overall, mPEG- b -PDMAEMA can be used as an efficient DNA vaccine vector which enhances adaptive immune responses by activating innate immunity.</description><identifier>ISSN: 0142-9612</identifier><identifier>EISSN: 1878-5905</identifier><identifier>DOI: 10.1016/j.biomaterials.2009.09.032</identifier><identifier>PMID: 19781770</identifier><language>eng</language><publisher>Netherlands: Elsevier Ltd</publisher><subject>Advanced Basic Science ; AIDS Vaccines - administration & dosage ; Animals ; Cytokines - biosynthesis ; Dentistry ; DNA - administration & dosage ; Electrophoresis, Agar Gel ; Enzyme-Linked Immunosorbent Assay ; Female ; Fluorescent Dyes ; Genes, gag ; Genetic Vectors ; HIV-1 ; HIV-1 - immunology ; Human immunodeficiency virus ; Immunity, Innate ; Macrophages - immunology ; Methacrylates - chemistry ; Mice ; Mice, Inbred BALB C ; Microscopy, Electron, Scanning ; Mucosa ; Mucous Membrane - metabolism ; Nylons - chemistry ; Particle Size ; PDMAEMA ; PEGylation ; Polyethylene Glycols - chemistry ; Vaccine ; Vector</subject><ispartof>Biomaterials, 2010-01, Vol.31 (1), p.115-123</ispartof><rights>Elsevier Ltd</rights><rights>2009 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c496t-95ea5ace8c3f689fc48419cf6298a1608dd4d3f52c852b0b324a183fa169a1c03</citedby><cites>FETCH-LOGICAL-c496t-95ea5ace8c3f689fc48419cf6298a1608dd4d3f52c852b0b324a183fa169a1c03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.biomaterials.2009.09.032$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19781770$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Qiao, Yong</creatorcontrib><creatorcontrib>Huang, Yang</creatorcontrib><creatorcontrib>Qiu, Chao</creatorcontrib><creatorcontrib>Yue, Xinye</creatorcontrib><creatorcontrib>Deng, Liandong</creatorcontrib><creatorcontrib>Wan, Yanmin</creatorcontrib><creatorcontrib>Xing, Jinfeng</creatorcontrib><creatorcontrib>Zhang, Congyou</creatorcontrib><creatorcontrib>Yuan, Songhua</creatorcontrib><creatorcontrib>Dong, Anjie</creatorcontrib><creatorcontrib>Xu, Jianqing</creatorcontrib><title>The use of PEGylated poly [2-(N,N-dimethylamino) ethyl methacrylate] as a mucosal DNA delivery vector and the activation of innate immunity and improvement of HIV-1-specific immune responses</title><title>Biomaterials</title><addtitle>Biomaterials</addtitle><description>Abstract To minimize the cytotoxicity of poly (2-(dimethylamino) ethyl methacrylate) (PDMAEMA) as a gene delivery vector, we synthesized PEGylated PDMAEMA by atom transfer radical polymerization (ATRP). Here we report its effects on transfection efficiency in vitro delivered with a GFP expression plasmid and immunogenicity in vivo after complexed with a HIV gag gene DNA vaccine. mPEG113 - b -PDMAEMA94 was efficient in condensing DNA and formed polyplexes with an average diameter of about 150 nm. The in vitro transfection experiments demonstrated that PEGylation dramatically decreased the cytotoxicity at the N / P ratios above 30, although the transfection efficiency in vitro was reduced. Interestingly, mice in vivo vaccination study clearly showed that PEGylated PDMAEMA used as DNA delivery vector significantly improved the prime effect of DNA vaccine through intranasal administration. Importantly, PEGylated PDMAEMA was further proved its ability to induce cytokines production by murine macrophages. Overall, mPEG- b -PDMAEMA can be used as an efficient DNA vaccine vector which enhances adaptive immune responses by activating innate immunity.</description><subject>Advanced Basic Science</subject><subject>AIDS Vaccines - administration & dosage</subject><subject>Animals</subject><subject>Cytokines - biosynthesis</subject><subject>Dentistry</subject><subject>DNA - administration & dosage</subject><subject>Electrophoresis, Agar Gel</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Female</subject><subject>Fluorescent Dyes</subject><subject>Genes, gag</subject><subject>Genetic Vectors</subject><subject>HIV-1</subject><subject>HIV-1 - immunology</subject><subject>Human immunodeficiency virus</subject><subject>Immunity, Innate</subject><subject>Macrophages - immunology</subject><subject>Methacrylates - chemistry</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Microscopy, Electron, Scanning</subject><subject>Mucosa</subject><subject>Mucous Membrane - metabolism</subject><subject>Nylons - chemistry</subject><subject>Particle Size</subject><subject>PDMAEMA</subject><subject>PEGylation</subject><subject>Polyethylene Glycols - chemistry</subject><subject>Vaccine</subject><subject>Vector</subject><issn>0142-9612</issn><issn>1878-5905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkltrFDEUxwdR7Fr9ChJ88ALOmstckj4Ipa1toayC1ReRkM2coVknyZrMDMyX87OZ2V1QfFDhQC7nd_7nkPyz7BnBS4JJ9WazXBtvVQ_BqC4uKcZiOQej97IF4TXPS4HL-9kCk4LmoiL0KHsU4wanMy7ow-yIiJqTusaL7MftHaAhAvIt-nBxOXVJtkFb303oC81frl6v8sZY6O9SxhrnX6HdHs1XSocd_xWpiBSyg_ZRdeh8dYoa6MwIYUIj6N4HpFyD-tRJ6d6MqjfezQ2Nc6kcGWsHZ_ppRxm7DX4EC66fkavrzznJ4xa0aY3eo4ACxK13EeLj7EGb3gCeHNbj7NO7i9uzq_zm_eX12elNrgtR9bkoQZVKA9esrbhodcELInRbUcEVqTBvmqJhbUk1L-karxktFOGsTTmhiMbsOHux103DfR8g9tKaqKHrlAM_RFmzgpBaMJrI538lWYkrVgr-T5ASUhSYkQSe7EEdfIwBWrkNxqowSYLlbAi5kb8bQs6GkHPsxnl66DKsLTS_Sg8OSMD5HoD0fKOBIKM24DQ0JqS_k403_9fn7R8yujPOaNV9gwnixg_BzTVERiqx_Dhbc3YmFkmkogX7CQbL5S0</recordid><startdate>20100101</startdate><enddate>20100101</enddate><creator>Qiao, Yong</creator><creator>Huang, Yang</creator><creator>Qiu, Chao</creator><creator>Yue, Xinye</creator><creator>Deng, Liandong</creator><creator>Wan, Yanmin</creator><creator>Xing, Jinfeng</creator><creator>Zhang, Congyou</creator><creator>Yuan, Songhua</creator><creator>Dong, Anjie</creator><creator>Xu, Jianqing</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T5</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7SR</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>F28</scope><scope>JG9</scope><scope>L7M</scope><scope>7X8</scope></search><sort><creationdate>20100101</creationdate><title>The use of PEGylated poly [2-(N,N-dimethylamino) ethyl methacrylate] as a mucosal DNA delivery vector and the activation of innate immunity and improvement of HIV-1-specific immune responses</title><author>Qiao, Yong ; Huang, Yang ; Qiu, Chao ; Yue, Xinye ; Deng, Liandong ; Wan, Yanmin ; Xing, Jinfeng ; Zhang, Congyou ; Yuan, Songhua ; Dong, Anjie ; Xu, Jianqing</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c496t-95ea5ace8c3f689fc48419cf6298a1608dd4d3f52c852b0b324a183fa169a1c03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Advanced Basic Science</topic><topic>AIDS Vaccines - administration & dosage</topic><topic>Animals</topic><topic>Cytokines - biosynthesis</topic><topic>Dentistry</topic><topic>DNA - administration & dosage</topic><topic>Electrophoresis, Agar Gel</topic><topic>Enzyme-Linked Immunosorbent Assay</topic><topic>Female</topic><topic>Fluorescent Dyes</topic><topic>Genes, gag</topic><topic>Genetic Vectors</topic><topic>HIV-1</topic><topic>HIV-1 - immunology</topic><topic>Human immunodeficiency virus</topic><topic>Immunity, Innate</topic><topic>Macrophages - immunology</topic><topic>Methacrylates - chemistry</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Microscopy, Electron, Scanning</topic><topic>Mucosa</topic><topic>Mucous Membrane - metabolism</topic><topic>Nylons - chemistry</topic><topic>Particle Size</topic><topic>PDMAEMA</topic><topic>PEGylation</topic><topic>Polyethylene Glycols - chemistry</topic><topic>Vaccine</topic><topic>Vector</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Qiao, Yong</creatorcontrib><creatorcontrib>Huang, Yang</creatorcontrib><creatorcontrib>Qiu, Chao</creatorcontrib><creatorcontrib>Yue, Xinye</creatorcontrib><creatorcontrib>Deng, Liandong</creatorcontrib><creatorcontrib>Wan, Yanmin</creatorcontrib><creatorcontrib>Xing, Jinfeng</creatorcontrib><creatorcontrib>Zhang, Congyou</creatorcontrib><creatorcontrib>Yuan, Songhua</creatorcontrib><creatorcontrib>Dong, Anjie</creatorcontrib><creatorcontrib>Xu, Jianqing</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Immunology Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Biomaterials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Qiao, Yong</au><au>Huang, Yang</au><au>Qiu, Chao</au><au>Yue, Xinye</au><au>Deng, Liandong</au><au>Wan, Yanmin</au><au>Xing, Jinfeng</au><au>Zhang, Congyou</au><au>Yuan, Songhua</au><au>Dong, Anjie</au><au>Xu, Jianqing</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The use of PEGylated poly [2-(N,N-dimethylamino) ethyl methacrylate] as a mucosal DNA delivery vector and the activation of innate immunity and improvement of HIV-1-specific immune responses</atitle><jtitle>Biomaterials</jtitle><addtitle>Biomaterials</addtitle><date>2010-01-01</date><risdate>2010</risdate><volume>31</volume><issue>1</issue><spage>115</spage><epage>123</epage><pages>115-123</pages><issn>0142-9612</issn><eissn>1878-5905</eissn><abstract>Abstract To minimize the cytotoxicity of poly (2-(dimethylamino) ethyl methacrylate) (PDMAEMA) as a gene delivery vector, we synthesized PEGylated PDMAEMA by atom transfer radical polymerization (ATRP). Here we report its effects on transfection efficiency in vitro delivered with a GFP expression plasmid and immunogenicity in vivo after complexed with a HIV gag gene DNA vaccine. mPEG113 - b -PDMAEMA94 was efficient in condensing DNA and formed polyplexes with an average diameter of about 150 nm. The in vitro transfection experiments demonstrated that PEGylation dramatically decreased the cytotoxicity at the N / P ratios above 30, although the transfection efficiency in vitro was reduced. Interestingly, mice in vivo vaccination study clearly showed that PEGylated PDMAEMA used as DNA delivery vector significantly improved the prime effect of DNA vaccine through intranasal administration. Importantly, PEGylated PDMAEMA was further proved its ability to induce cytokines production by murine macrophages. Overall, mPEG- b -PDMAEMA can be used as an efficient DNA vaccine vector which enhances adaptive immune responses by activating innate immunity.</abstract><cop>Netherlands</cop><pub>Elsevier Ltd</pub><pmid>19781770</pmid><doi>10.1016/j.biomaterials.2009.09.032</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0142-9612 |
| ispartof | Biomaterials, 2010-01, Vol.31 (1), p.115-123 |
| issn | 0142-9612 1878-5905 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_734117932 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Advanced Basic Science AIDS Vaccines - administration & dosage Animals Cytokines - biosynthesis Dentistry DNA - administration & dosage Electrophoresis, Agar Gel Enzyme-Linked Immunosorbent Assay Female Fluorescent Dyes Genes, gag Genetic Vectors HIV-1 HIV-1 - immunology Human immunodeficiency virus Immunity, Innate Macrophages - immunology Methacrylates - chemistry Mice Mice, Inbred BALB C Microscopy, Electron, Scanning Mucosa Mucous Membrane - metabolism Nylons - chemistry Particle Size PDMAEMA PEGylation Polyethylene Glycols - chemistry Vaccine Vector |
| title | The use of PEGylated poly [2-(N,N-dimethylamino) ethyl methacrylate] as a mucosal DNA delivery vector and the activation of innate immunity and improvement of HIV-1-specific immune responses |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-19T10%3A18%3A30IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20use%20of%20PEGylated%20poly%20%5B2-(N,N-dimethylamino)%20ethyl%20methacrylate%5D%20as%20a%20mucosal%20DNA%20delivery%20vector%20and%20the%20activation%20of%20innate%20immunity%20and%20improvement%20of%20HIV-1-specific%20immune%20responses&rft.jtitle=Biomaterials&rft.au=Qiao,%20Yong&rft.date=2010-01-01&rft.volume=31&rft.issue=1&rft.spage=115&rft.epage=123&rft.pages=115-123&rft.issn=0142-9612&rft.eissn=1878-5905&rft_id=info:doi/10.1016/j.biomaterials.2009.09.032&rft_dat=%3Cproquest_cross%3E35063598%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=21144031&rft_id=info:pmid/19781770&rft_els_id=1_s2_0_S0142961209009624&rfr_iscdi=true |