Development of bovine embryos in a cell-free culture medium: Effects of type of serum, timing of its inclusion and heat inactivation

Development of bovine embryos in a cell-free culture medium: Effects of type of serum, timing of its inclusion and heat inactivation

Bovine embryos, derived from in vitro matured (IVM)/in vitro fertilized (IVF) ova, were used to investigate the effects of timing of serum inclusion in the culture medium and different types of blood sera and heat inactivation of the serum on embryo development. In Experiment 1, oocytes at 18 h post...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Theriogenology 1994, Vol.41 (6), p.1241-1249
Hauptverfasser: Pinyopummintr, T., Bavister, B.D.
Format: Artikel
Sprache:eng
Schlagworte:
BOVIN
bovine embryos
cell-free medium
CULTIVO DE EMBRIONES
CULTURE D'EMBRYON
GANADO BOVINO
heat-inactivation
in vitro fertilization
MEDIO DE CULTIVO
MILIEU DE CULTURE
serum
SERUM SANGUIN
SUERO SANGUINEO
TRAITEMENT THERMIQUE
TRATAMIENTO TERMICO
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 1249
container_issue 6
container_start_page 1241
container_title Theriogenology
container_volume 41
creator Pinyopummintr, T.
Bavister, B.D.
description Bovine embryos, derived from in vitro matured (IVM)/in vitro fertilized (IVF) ova, were used to investigate the effects of timing of serum inclusion in the culture medium and different types of blood sera and heat inactivation of the serum on embryo development. In Experiment 1, oocytes at 18 h post insemination were allocated to 1 of the following 4 treatments: 1) TCM-199 + 0.1 mg/ml polyvinylalcohol (PVA), 2) TCM-199 supplemented with 10% bovine calf serum (BCS), 3) PVA medium followed by BCS medium at 47 h, or 4) PVA medium followed by BCS medium at 82 h. Supplementation with BCS at 18 h post insemination suppressed (P
doi_str_mv 10.1016/0093-691X(94)90481-W
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_734108697</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>0093691X9490481W</els_id><sourcerecordid>734108697</sourcerecordid><originalsourceid>FETCH-LOGICAL-c444t-15acbd712f923cfc44066ba96b6b02584acecd6571054593e94ce1fef9b04e2d3</originalsourceid><addsrcrecordid>eNp9kFFLHDEUhUNRdN36B0Qkb1VwbDKTSTZ9KBSrVhD60Ip9C5nMjUZmJmuSWdj3_vBm3EXf-nTh3HPO5X4IHVNyQQnlnwmRVcEl_XMq2ZkkbEGLhw9oRhdCFlVZ0R00e7Pso4MYnwkhFed0D-1TLkrBhJihv99hBZ1f9jAk7C1u_MoNgKFvwtpH7AassYGuK2wAwGbs0hgA99C6sf-Cr6wFk-IUTOslTDNCGPtznFzvhsdJcGmqMd0Ync9tQ4ufQKcsaZPcSqesfkS7VncRDrdzju6vr35f_ijuft7cXn67KwxjLBW01qZpBS2tLCtjs0g4b7TkDW9IWS-YNmBaXgtKalbLCiQzQC1Y2RAGZVvN0adN7zL4lxFiUr2L03d6AD9GJSpGyYJLkZ1s4zTBxxjAqmVwvQ5rRYma8KuJrZrYKsnUK371kGMn2wNjkxm9h7a8s-FoY7DaK_0YXFT3vySryzq_NEdfN0vIDFYOgorGwWAy7JAxq9a7_5__B_5onx0</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>734108697</pqid></control><display><type>article</type><title>Development of bovine embryos in a cell-free culture medium: Effects of type of serum, timing of its inclusion and heat inactivation</title><source>Elsevier ScienceDirect Journals</source><creator>Pinyopummintr, T. ; Bavister, B.D.</creator><creatorcontrib>Pinyopummintr, T. ; Bavister, B.D.</creatorcontrib><description>Bovine embryos, derived from in vitro matured (IVM)/in vitro fertilized (IVF) ova, were used to investigate the effects of timing of serum inclusion in the culture medium and different types of blood sera and heat inactivation of the serum on embryo development. In Experiment 1, oocytes at 18 h post insemination were allocated to 1 of the following 4 treatments: 1) TCM-199 + 0.1 mg/ml polyvinylalcohol (PVA), 2) TCM-199 supplemented with 10% bovine calf serum (BCS), 3) PVA medium followed by BCS medium at 47 h, or 4) PVA medium followed by BCS medium at 82 h. Supplementation with BCS at 18 h post insemination suppressed (P&lt;0.05) development of morulae/blastocysts (17.6%) when compared with PVA (30.5%) or with serum supplementation at 47 or 82 h post insemination (32.4 and 27.6%, respectively). However, inclusion of BCS at 18, 47 or 82 h post insemination produced more blastocysts (16.8, 29.3 and 22.1%, respectively; P&lt;0.05) than medium +PVA (8.8%). In Experiment 2, ova were cultured from 18 h to 42 h post insemination in PVA-medium, then ≥2-cell embryos were transferred into serum-supplemented medium for another 168 h. Fetal bovine serum (FBS) ± heat-inactivation (56°C for 30 min, = heated FBS) suppressed morula/blastocyst development compared with medium + PVA, medium + BCS or medium + heated BCS (P&lt;0.05). Bovine calf serum was superior to FBS in supporting blastocyst development (35.1 and 15.2%, respectively), but there was no difference between BCS and heated BCS. However, heated FBS increased the proportion of blastocysts/≥8-cell embryos compared with that of FBS (51.0 and 31.4%, respectively; P&lt;0.05). These results indicate that the type of serum supplementation and the timing of its inclusion in the culture medium markedly affect bovine embryo development in vitro, and that heat inactivation of serum with high embryotrophic properties is not necessary.</description><identifier>ISSN: 0093-691X</identifier><identifier>EISSN: 1879-3231</identifier><identifier>DOI: 10.1016/0093-691X(94)90481-W</identifier><identifier>PMID: 16727477</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>BOVIN ; bovine embryos ; cell-free medium ; CULTIVO DE EMBRIONES ; CULTURE D'EMBRYON ; GANADO BOVINO ; heat-inactivation ; in vitro fertilization ; MEDIO DE CULTIVO ; MILIEU DE CULTURE ; serum ; SERUM SANGUIN ; SUERO SANGUINEO ; TRAITEMENT THERMIQUE ; TRATAMIENTO TERMICO</subject><ispartof>Theriogenology, 1994, Vol.41 (6), p.1241-1249</ispartof><rights>1994</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c444t-15acbd712f923cfc44066ba96b6b02584acecd6571054593e94ce1fef9b04e2d3</citedby><cites>FETCH-LOGICAL-c444t-15acbd712f923cfc44066ba96b6b02584acecd6571054593e94ce1fef9b04e2d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0093-691X(94)90481-W$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3538,4011,27905,27906,27907,45977</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16727477$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pinyopummintr, T.</creatorcontrib><creatorcontrib>Bavister, B.D.</creatorcontrib><title>Development of bovine embryos in a cell-free culture medium: Effects of type of serum, timing of its inclusion and heat inactivation</title><title>Theriogenology</title><addtitle>Theriogenology</addtitle><description>Bovine embryos, derived from in vitro matured (IVM)/in vitro fertilized (IVF) ova, were used to investigate the effects of timing of serum inclusion in the culture medium and different types of blood sera and heat inactivation of the serum on embryo development. In Experiment 1, oocytes at 18 h post insemination were allocated to 1 of the following 4 treatments: 1) TCM-199 + 0.1 mg/ml polyvinylalcohol (PVA), 2) TCM-199 supplemented with 10% bovine calf serum (BCS), 3) PVA medium followed by BCS medium at 47 h, or 4) PVA medium followed by BCS medium at 82 h. Supplementation with BCS at 18 h post insemination suppressed (P&lt;0.05) development of morulae/blastocysts (17.6%) when compared with PVA (30.5%) or with serum supplementation at 47 or 82 h post insemination (32.4 and 27.6%, respectively). However, inclusion of BCS at 18, 47 or 82 h post insemination produced more blastocysts (16.8, 29.3 and 22.1%, respectively; P&lt;0.05) than medium +PVA (8.8%). In Experiment 2, ova were cultured from 18 h to 42 h post insemination in PVA-medium, then ≥2-cell embryos were transferred into serum-supplemented medium for another 168 h. Fetal bovine serum (FBS) ± heat-inactivation (56°C for 30 min, = heated FBS) suppressed morula/blastocyst development compared with medium + PVA, medium + BCS or medium + heated BCS (P&lt;0.05). Bovine calf serum was superior to FBS in supporting blastocyst development (35.1 and 15.2%, respectively), but there was no difference between BCS and heated BCS. However, heated FBS increased the proportion of blastocysts/≥8-cell embryos compared with that of FBS (51.0 and 31.4%, respectively; P&lt;0.05). These results indicate that the type of serum supplementation and the timing of its inclusion in the culture medium markedly affect bovine embryo development in vitro, and that heat inactivation of serum with high embryotrophic properties is not necessary.</description><subject>BOVIN</subject><subject>bovine embryos</subject><subject>cell-free medium</subject><subject>CULTIVO DE EMBRIONES</subject><subject>CULTURE D'EMBRYON</subject><subject>GANADO BOVINO</subject><subject>heat-inactivation</subject><subject>in vitro fertilization</subject><subject>MEDIO DE CULTIVO</subject><subject>MILIEU DE CULTURE</subject><subject>serum</subject><subject>SERUM SANGUIN</subject><subject>SUERO SANGUINEO</subject><subject>TRAITEMENT THERMIQUE</subject><subject>TRATAMIENTO TERMICO</subject><issn>0093-691X</issn><issn>1879-3231</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><recordid>eNp9kFFLHDEUhUNRdN36B0Qkb1VwbDKTSTZ9KBSrVhD60Ip9C5nMjUZmJmuSWdj3_vBm3EXf-nTh3HPO5X4IHVNyQQnlnwmRVcEl_XMq2ZkkbEGLhw9oRhdCFlVZ0R00e7Pso4MYnwkhFed0D-1TLkrBhJihv99hBZ1f9jAk7C1u_MoNgKFvwtpH7AassYGuK2wAwGbs0hgA99C6sf-Cr6wFk-IUTOslTDNCGPtznFzvhsdJcGmqMd0Ync9tQ4ufQKcsaZPcSqesfkS7VncRDrdzju6vr35f_ijuft7cXn67KwxjLBW01qZpBS2tLCtjs0g4b7TkDW9IWS-YNmBaXgtKalbLCiQzQC1Y2RAGZVvN0adN7zL4lxFiUr2L03d6AD9GJSpGyYJLkZ1s4zTBxxjAqmVwvQ5rRYma8KuJrZrYKsnUK371kGMn2wNjkxm9h7a8s-FoY7DaK_0YXFT3vySryzq_NEdfN0vIDFYOgorGwWAy7JAxq9a7_5__B_5onx0</recordid><startdate>1994</startdate><enddate>1994</enddate><creator>Pinyopummintr, T.</creator><creator>Bavister, B.D.</creator><general>Elsevier Inc</general><scope>FBQ</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1994</creationdate><title>Development of bovine embryos in a cell-free culture medium: Effects of type of serum, timing of its inclusion and heat inactivation</title><author>Pinyopummintr, T. ; Bavister, B.D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c444t-15acbd712f923cfc44066ba96b6b02584acecd6571054593e94ce1fef9b04e2d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>BOVIN</topic><topic>bovine embryos</topic><topic>cell-free medium</topic><topic>CULTIVO DE EMBRIONES</topic><topic>CULTURE D'EMBRYON</topic><topic>GANADO BOVINO</topic><topic>heat-inactivation</topic><topic>in vitro fertilization</topic><topic>MEDIO DE CULTIVO</topic><topic>MILIEU DE CULTURE</topic><topic>serum</topic><topic>SERUM SANGUIN</topic><topic>SUERO SANGUINEO</topic><topic>TRAITEMENT THERMIQUE</topic><topic>TRATAMIENTO TERMICO</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pinyopummintr, T.</creatorcontrib><creatorcontrib>Bavister, B.D.</creatorcontrib><collection>AGRIS</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Theriogenology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pinyopummintr, T.</au><au>Bavister, B.D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of bovine embryos in a cell-free culture medium: Effects of type of serum, timing of its inclusion and heat inactivation</atitle><jtitle>Theriogenology</jtitle><addtitle>Theriogenology</addtitle><date>1994</date><risdate>1994</risdate><volume>41</volume><issue>6</issue><spage>1241</spage><epage>1249</epage><pages>1241-1249</pages><issn>0093-691X</issn><eissn>1879-3231</eissn><abstract>Bovine embryos, derived from in vitro matured (IVM)/in vitro fertilized (IVF) ova, were used to investigate the effects of timing of serum inclusion in the culture medium and different types of blood sera and heat inactivation of the serum on embryo development. In Experiment 1, oocytes at 18 h post insemination were allocated to 1 of the following 4 treatments: 1) TCM-199 + 0.1 mg/ml polyvinylalcohol (PVA), 2) TCM-199 supplemented with 10% bovine calf serum (BCS), 3) PVA medium followed by BCS medium at 47 h, or 4) PVA medium followed by BCS medium at 82 h. Supplementation with BCS at 18 h post insemination suppressed (P&lt;0.05) development of morulae/blastocysts (17.6%) when compared with PVA (30.5%) or with serum supplementation at 47 or 82 h post insemination (32.4 and 27.6%, respectively). However, inclusion of BCS at 18, 47 or 82 h post insemination produced more blastocysts (16.8, 29.3 and 22.1%, respectively; P&lt;0.05) than medium +PVA (8.8%). In Experiment 2, ova were cultured from 18 h to 42 h post insemination in PVA-medium, then ≥2-cell embryos were transferred into serum-supplemented medium for another 168 h. Fetal bovine serum (FBS) ± heat-inactivation (56°C for 30 min, = heated FBS) suppressed morula/blastocyst development compared with medium + PVA, medium + BCS or medium + heated BCS (P&lt;0.05). Bovine calf serum was superior to FBS in supporting blastocyst development (35.1 and 15.2%, respectively), but there was no difference between BCS and heated BCS. However, heated FBS increased the proportion of blastocysts/≥8-cell embryos compared with that of FBS (51.0 and 31.4%, respectively; P&lt;0.05). These results indicate that the type of serum supplementation and the timing of its inclusion in the culture medium markedly affect bovine embryo development in vitro, and that heat inactivation of serum with high embryotrophic properties is not necessary.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>16727477</pmid><doi>10.1016/0093-691X(94)90481-W</doi><tpages>9</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0093-691X
ispartof Theriogenology, 1994, Vol.41 (6), p.1241-1249
issn 0093-691X
1879-3231
language eng
recordid cdi_proquest_miscellaneous_734108697
source Elsevier ScienceDirect Journals
subjects BOVIN
bovine embryos
cell-free medium
CULTIVO DE EMBRIONES
CULTURE D'EMBRYON
GANADO BOVINO
heat-inactivation
in vitro fertilization
MEDIO DE CULTIVO
MILIEU DE CULTURE
serum
SERUM SANGUIN
SUERO SANGUINEO
TRAITEMENT THERMIQUE
TRATAMIENTO TERMICO
title Development of bovine embryos in a cell-free culture medium: Effects of type of serum, timing of its inclusion and heat inactivation
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-17T08%3A58%3A15IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Development%20of%20bovine%20embryos%20in%20a%20cell-free%20culture%20medium:%20Effects%20of%20type%20of%20serum,%20timing%20of%20its%20inclusion%20and%20heat%20inactivation&rft.jtitle=Theriogenology&rft.au=Pinyopummintr,%20T.&rft.date=1994&rft.volume=41&rft.issue=6&rft.spage=1241&rft.epage=1249&rft.pages=1241-1249&rft.issn=0093-691X&rft.eissn=1879-3231&rft_id=info:doi/10.1016/0093-691X(94)90481-W&rft_dat=%3Cproquest_cross%3E734108697%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=734108697&rft_id=info:pmid/16727477&rft_els_id=0093691X9490481W&rfr_iscdi=true