In vitro methods of assessing the viability of rainbow trout spermatozoa

In vitro methods of assessing the viability of rainbow trout spermatozoa

The accuracy of three in vitro methods for estimating the proportion of dead rainbow trout ( Onchorhynchus mykiss ) spermatozoa was investigated. Motility rating, fluorometry using ethidium bromide, and lactate dehydrogenase (LDH) activity in seminal plasma were compared. Semen samples were prepared...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Theriogenology 1992-10, Vol.38 (4), p.679-686
Hauptverfasser: McNiven, M.A., Gallant, R.K., Richardson, G.F.
Format: Artikel
Sprache:eng
Schlagworte:
ESPERMATOZOO
EXPERIMENTACION IN VITRO
EXPERIMENTATION EN LABORATOIRE
EXPERIMENTATION IN VITRO
EXPERIMENTOS EN LABORATORIO
FERTILIDAD
FERTILITE
FLUORIMETRIA
FLUORIMETRIE
fluorometry
lactate dehydrogenase
LACTATE DESHYDROGENASE
LACTATO DESHIDROGENASA
LDH
MACHO
MALE
METHODE
METODOS
motility
MOUVEMENT
MOVIMIENTO
SALMO GAIRDNERI
sperm viability
SPERMATOZOIDE
VIABILIDAD
VIABILITE
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:The accuracy of three in vitro methods for estimating the proportion of dead rainbow trout ( Onchorhynchus mykiss ) spermatozoa was investigated. Motility rating, fluorometry using ethidium bromide, and lactate dehydrogenase (LDH) activity in seminal plasma were compared. Semen samples were prepared to contain 0, 25, 50, 75 and 100% killed spermatozoa. All three methods demonstrated highly significant relationships (P
ISSN:0093-691X
1879-3231
DOI:10.1016/0093-691X(92)90030-U